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Therapeutic effects of tyroserleutide on lung metastasis of human hepatocellular carcinoma SK-HEP-1 and its mechanism affecting ICAM-1 and MMP-2 and -9

Authors Che X, Lu R, Fu Z, Sun Y, Zhu Z, Li J, Wang S, Jia J, Wang Q, Yao Z

Received 14 June 2018

Accepted for publication 23 August 2018

Published 9 October 2018 Volume 2018:12 Pages 3357—3368

DOI https://doi.org/10.2147/DDDT.S177126

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Anastasios Lymperopoulos


Xuchun Che,1,* Rong Lu,1,2,* Zheng Fu,2 Yajun Sun,3 Zhi-feng Zhu,2 Jin-ping Li,2 Song Wang,2 Jing Jia,2 Qing Wang,4 Zhi Yao1

1Department of Immunology, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry of China, School of Medical Sciences, Tianjin Medical University, Tianjin, People’s Republic of China; 2Department of Drug Development, Tianjin Kangzhe Pharmaceutical Company, Ltd., Tianjin, People’s Republic of China; 3Department of Blood Transfusion, General Hospital, Tianjin Medical University, Tianjin, People’s Republic of China; 4Department of Clinical Laboratory, General Hospital, Tianjin Medical University, Tianjin, People’s Republic of China

*These authors contributed equally to this work

Background: Tyroserleutide (YSL) inhibits the growth and metastasis of human hepatocellular carcinoma (HCC). This paper studied the effect of YSL on metastasis of human HCC and investigated its mechanisms.
Methods: In vivo, experimental lung metastasis models of human HCC SK-HEP-1 cells in nude mice were established, and In vitro, the proliferation, adhesion and invasion of SK-HEP-1 cells were detected.
Results: In vivo, YSL significantly inhibited the metastasis of human HCC. In vitro, YSL significantly inhibited the proliferation, adhesion and invasion of SK-HEP-1 cells. Through analyses with reverse transcription PCR (RT-PCR) and Western blot, we observed that YSL significantly inhibited the expressions of ICAM-1 in SK-HEP-1 cells. Through RT-PCR, Western blot and zymography methods, YSL was discovered to decrease the mRNA level, protein expression and activity of MMP-2 and -9 in SK-HEP-1 cells.
Conclusion: We concluded that YSL could inhibit tumor growth and metastasis of human HCC SK-HEP-1 cells.

Keywords: proliferation, adhesion, invasion, ICAM-1, MMP-2, MMP-9

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