The Long Noncoding RNA LINC00908 Facilitates Hepatocellular Carcinoma Progression Via Interaction With Sox-4
Authors Hu X, Li Q, Zhang J
Received 23 May 2019
Accepted for publication 7 August 2019
Published 30 September 2019 Volume 2019:11 Pages 8789—8797
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 4
Editor who approved publication: Dr Eileen O'Reilly
Xinhua Hu, Qingxiang Li, Jinfeng Zhang
Department of Laboratory Medicine, Juxian Hospital of Traditional Chinese Medicine, Rizhao 276500, Shandong, People’s Republic of China
Correspondence: Jinfeng Zhang
Department of Laboratory Medicine, Juxian Hospital of Traditional Chinese Medicine, No. 338, South Chengyang Road, Rizhao 276500, Rizhao, People’s Republic of China
Tel +86 633 6887275
Fax +86 633 6887275
Background: The hepatocellular carcinoma (HCC) is a highly aggressive and common malignancy worldwide. Accumulating evidence has demonstrated a pivotal role of long noncoding RNAs (lncRNAs) in various tumors. However, the function of intergenic lncRNA LINC00908 is still unknown in HCC.
Methods: The RT-qPCR method was used to quantify the expression of LINC00908. Migration and viability assay were performed to evaluate the in vitro effect and xenograft tumor model was used to measure the in vivo effect. Immunoblot was used to identify the association of LINC00908 with Sox-4 and the stability of Sox-4.
Results: We found a novel lncRNA related to HCC. LINC00908 is highly expressed in tumorous tissues and cell lines compared with normal ones. High LINC00908 expression correlated with advanced TNM stages, tumor size and metastasis. LINC00908 promoted the migration and viability of HCC cells. The in vivo xenograft tumor growth and proliferation were also enhanced by LINC00908 overexpression and inhibited by LINC00908 silence. LINC00908 physically interacted with Sox-4, and the association between LINC00908 and Sox-4 increased the stability of Sox-4 by reducing proteasomal degradation.
Conclusion: Taken together, our current work has identified a novel lncRNA LINC00908 which is critically involved in HCC progression. The LINC00908-Sox-4 axis might provide a new and potential target for pharmaceutical therapies.
Keywords: lncRNA, LINC00908, Sox-4, HCC
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