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Risk analysis of systemic levels of estrogen and adipokines as well as estrogen receptors from PBMCs in childbearing and perimenopausal women with obesity

Authors Fan H, Chen S, Gao B, Ding S, Zhao Q, Li C, Asakawa T

Received 20 February 2019

Accepted for publication 4 July 2019

Published 1 August 2019 Volume 2019:12 Pages 1287—1295

DOI https://doi.org/10.2147/DMSO.S206069

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 2

Editor who approved publication: Dr Muthuswamy Balasubramanyam


Huijuan Fan,*,1 Shujiao Chen,*,1,2 Bizhen Gao,1 Shanshan Ding,1 Qiang Zhao,1 Candong Li,1 Tetsuya Asakawa1,3

1Research Base of Traditional Chinese Medicine Syndrome, Fujian University of Traditional Chinese Medicine, Fuzhou, Shangjie Minhou 350122, People’s Republic of China; 2Department of Internal Medicine, The Third People’s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, Shangjie Minhou 350122, People’s Republic of China; 3Department of Neurosurgery, Hamamatsu University School of Medicine, Hamamatsu-city, Shizuoka 431-3192, Japan

*These authors contributed equally to this work

Purpose: We aimed to evaluate the clinical value of systemic levels of estrogen and adipokines as well as estrogen receptors from peripheral blood mononuclear cells (PBMCs) in childbearing and perimenopausal women with obesity.
Subjects and methods: We observed 292 women, including 160 perimenopausal women (80 with obesity and 80 without obesity) and 132 women of childbearing age (67 with obesity and 65 without obesity). Body parameters, such as body mass index and waist circumference, were measured. Fat distribution was evaluated using a computerized tomography scanner. The levels of serum estrogen, leptin, visfatin, and adiponectin were measured using an enzyme-linked immunosorbent assay. The expression of circulating ERs was evaluated by Western blot analysis.
Results: Perimenopausal women and childbearing women with obesity exhibited lower levels of estrogen and adiponectin, in addition to a distribution of visceral fat with higher levels of leptin and visfatin. These findings reflect the current data of menopausal women, which confirms the reliability of this experimental system. However, the expression of ERα in peripheral blood was significantly enhanced in women with obesity of both childbearing and perimenopausal age. This result is contrary to the common understanding of adipose tissue, namely that ERα is protective. The expression of ERβ in the women without obesity of both childbearing and perimenopausal age was higher than in women with obesity, which coincides with the results of a previous study on adipose tissue.
Conclusion: Our data fundamentally contradicts the utility of circulating ERα and ERα/ERβ evaluations in obesity studies. Because estrogen exerts pleiotropic effects on multiple tissues in the body through differential regulation of ERs, although the expression of ERβ coincides with the results of a previous study on adipose tissue, the expression levels of ERs in blood cannot be used as a diagnostic of informative tool for obesity in women.

Keywords: estrogen receptors, obesity, estrogen, fat distribution, adipokines, perimenopause


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