Back to Journals » Veterinary Medicine: Research and Reports » Volume 1

Parasitological detection and molecular evidence of Hepatozoon canis from canines in Manila, Philippines

Authors Baticados A, Baticados W, Carlos Sr. E, Carlos, Villarba, Subiaga, Baticados W

Published 5 January 2011 Volume 2010:1 Pages 7—10


Review by Single anonymous peer review

Peer reviewer comments 2

Abigail M Baticados1, Waren N Baticados1, Enrique T Carlos2, Sixto MEAS Carlos2, Lorelie A Villarba1, Sherlyn G Subiaga1, Jomarte M Magcalas1
1Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, Philippines; 2Makati Dog and Cat Hospital (MDCH), Makati City, Philippines

Abstract: Hepatozoon canis infection in canines is allegedly an underreported disease in the Philippines. In over the past four decades, there are only two published cases in the country. A total of 168 canine blood samples was processed for polymerase chain reaction (PCR) amplification and blood parasite examination (BPE). The PCR method was able to provide molecular evidence for the presence of Hepatozoon canis genomic DNA in one sample (0.6%). Consequently, Hepatozoon canis gametocytes demonstrating the classical elongated appearance in leucocytes were also consistently seen on Giemsa®-stained blood smears of the PCR-positive animal after BPE. The study elucidates the parasitological detection and first molecular evidence of Hepatozoon canis infection in the Philippines by PCR.

Keywords: Hepatozoon canis, Manila, polymerase chain reaction, blood parasite examination

Creative Commons License © 2011 The Author(s). This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.