Back to Journals » International Journal of Nanomedicine » Volume 15

Oxygen Functional Groups on MWCNT Surface as Critical Factor Boosting T2 Relaxation Rate of Water Protons: Towards Improved CNT-Based Contrast Agents

Authors Tomczyk MM, Boncel S, Herman A, Krawczyk T, Jakóbik-Kolon A, Pawlyta M, Krzywiecki M, Chrobak A, Minoshima M, Sugihara F, Kikuchi K, Kuźnik N

Received 14 April 2020

Accepted for publication 18 August 2020

Published 6 October 2020 Volume 2020:15 Pages 7433—7450

DOI https://doi.org/10.2147/IJN.S257230

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Anderson Oliveira Lobo


Mateusz Michał Tomczyk,1 Sławomir Boncel,1 Artur Herman,2 Tomasz Krawczyk,1 Agata Jakóbik-Kolon,1 Mirosława Pawlyta,3 Maciej Krzywiecki,4 Artur Chrobak,5 Masafumi Minoshima,6 Fuminori Sugihara,7 Kazuya Kikuchi,6 Nikodem Kuźnik1

1Faculty of Chemistry, Silesian University of Technology, Gliwice, Poland; 2Department of Semiconductor Materials Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology, Wrocław, Poland; 3Faculty of Mechanical Engineering, Silesian University of Technology, Gliwice, Poland; 4Institute of Physics – Center for Science and Education, Silesian University of Technology, Gliwice, Poland; 5August Chełkowski Institute of Physics, University of Silesia, Katowice, Poland; 6Graduate School of Engineering, Osaka University, Osaka, Japan; 7Immunology Frontier Research Center, Osaka University, Osaka, Japan

Correspondence: Nikodem Kuźnik; Sławomir Boncel Email nikodem.kuznik@polsl.pl; slawomir.boncel@polsl.pl

Purpose: Salicyl (Sal) – among other oxygen functionalities – multi-walled carbon nanotubes (MWCNTs) and their nanohybrids are investigated as promising contrast agents (CA) in magnetic resonance imaging (MRI) or drug delivery platforms, due to their unique properties. The preliminary results and the literature reports were the motivation to endow high r2 relaxivities, excellent dispersibility in water, and biocompatibility to superparamagnetic MWCNTs nanohybrids. It was hypothesized that these goals could be achieved by, not described in the literature yet, two-stage oxygen functionalization of MWCNTs.
Results: Two structurally different MWCNT materials differing in diameters (44 and 12 nm) and the iron content (4.7% and 0.5%) are studied toward the functionalization effect on the T2 relaxometric properties. MWCNT oxidation is typically the first step of functionalization resulting in “first generation” oxygen functional groups (OFGs) on the surface. Until now, the impact of OFGs on the relaxivity of MWCNT was not truly recognized, but this study sheds light on this issue. By follow-up functionalization of oxidized MWCNT with 4-azidosalicylic acid through [2+1] cycloaddition of the corresponding nitrene, “second generation” of oxygen functional groups is grafted onto the nanohybrid, ie, Sal functionality.
Conclusion: The introduced OFGs are responsible for an almost 30% increase in the relaxivity, which leads to remarkable r2 relaxivity of 951 mM− 1s− 1 (419 (mg/mL)− 1s− 1), the unprecedented value reported to date for this class of CAs. Also, the resulting nanohybrids express low cytotoxicity and superb diffusion after subcutaneous injection to a mouse.

Keywords: multi-walled carbon nanotubes, magnetic resonance imaging, MRI contrast agents, carbon nanotube functionalization, T2 relaxivity

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]