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Nudol, a phenanthrene derivative from Dendrobium nobile, induces cell cycle arrest and apoptosis and inhibits migration in osteosarcoma cells

Authors Zhang Y, Zhang Q, Xin W, Liu N, Zhang H

Received 13 November 2018

Accepted for publication 10 June 2019

Published 29 July 2019 Volume 2019:13 Pages 2591—2601

DOI https://doi.org/10.2147/DDDT.S180610

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Professor Jianbo Sun


Yuying Zhang,1 Qianqian Zhang,1 Wei Xin,2 Na Liu,1 Hua Zhang1

1School of Biological Science and Technology, University of Jinan, Jinan, People’s Republic of China; 2Central Laboratory, Shandong Provincial Hospital, Shandong University, Jinan, People’s Republic of China

Purpose: Osteosarcoma is the most common malignancy of the bone in children and adolescents. There is an urgent need for the development of novel drugs to treat it. Nudol(1), a phenanthrene compound from the traditional Chinese medicine, Dendrobium nobile, exhibited antiproliferative activity against osteosarcoma cells. Therefore, the aim of the present study was to investigate the role and underlying mechanism of nudol(1) as potential chemotherapy for osteosarcoma.
Methods: Cell viability was determined by MTT assay. Cell-cycle phase distribution was analyzed by flow cytometry and Western blot. DAPI staining was used for morphology observation. Apoptosis was analysis via flow cytometry. The expression levels of mRNA and protein related to capase-mediated apoptotic pathway were detected by real-time PCR and western blotting. Migration was determined by wound healing assays.
Results: Nudol(1) significantly decreased cell viability in several cancer cell lines. Moreover, nudol(1) caused cell cycle arrest at G2/M phase in U2OS cells, and it also induced cell apoptosis through the caspase-dependent pathway. In addition, treatment with nudol(1) suppressed the migration of U2OS cells.
Conclusion: The present study, for the first time, demonstrated effects of nudol(1) on OS in vitro and the potential molecular mechanisms. Accordingly, nudol(1) might have the potential for further development as a lead compound against bone tumor.

Keywords: nudol(1), phenanthrene, Dendrobium nobile, anti-proliferation, osteosarcoma

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