MiR-802 Suppresses Colorectal Cancer Cell Viability, Migration and Invasion by Targeting RAN
Authors Feng H, Liu L, Xu L, Wang H, Hua Q, He P
Received 19 September 2019
Accepted for publication 21 February 2020
Published 26 March 2020 Volume 2020:12 Pages 2291—2300
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Professor Harikrishna Nakshatri
Hailong Feng,1 Lingling Liu,1 Laijing Xu,1 Haili Wang,1 Qiuju Hua,2 Peng He1
1Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui City, Henan Province 453100, People’s Republic of China; 2Hospital of Nephrology, The First Affiliated Hospital of Xinxiang Medical University, Weihui City, Henan Province 453100, People’s Republic of China
Correspondence: Peng He
Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui City, Henan Province 453100, People’s Republic of China
Purpose: Colorectal cancer is one of the most malignant tumors in the world, and the incidence is increasing every year. MicroRNAs (miRNA) are small non-coding RNAs that are involved in a variety of physiological or pathological processes. Abnormal expression of microRNA-802 (miR-802) has been demonstrated in various types of cancer. However, the expression and biological role of miR-802 in human colorectal cancer remain largely unknown.
Methods: Here, we used quantitative real-time PCR (qRT-PCR) to measure miR-802 expression levels in colorectal cancer tissues and cell lines. Cell Counting Kit-8 (CCK-8) was used to assess the effect of miR-802 on colorectal cancer cell viability. Migration and invasion assays were performed to determine the effect of miR-802 on metastasis of colon tumor cells by transwell analysis. Luciferase activity assays were used to confirm the target of miR-802.
Results: The results show that miR-802 is significantly downregulated in colorectal cancer tissues and cell lines. Overexpression of miR-802 profoundly inhibited viability, migration and invasion of colorectal cancer cells. In addition, we have newly discovered that the Ras-associated nucleus (RAN) is a direct target of miR-802 which could reverse the effects induced by miR-802 overexpression in colorectal cancer cells.
Conclusion: In conclusion, our study shows that miR-802 is downregulated in colorectal cancer, and overexpression of miR-802 inhibits colorectal cancer cell viability, migration and invasion by directly targeting RAN.
Keywords: miR-802, colorectal cancer, RAN, viability, migration, invasion
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