MicroRNA-140-5p is Downregulated in Osteosarcoma and Overexpression of MicroRNA-140-5p Inhibits Cancer Cell Proliferation by Downregulating GLUT-1
Authors Geng X, Wang H, Xu L, Han Y, Liu Y
Received 3 November 2020
Accepted for publication 20 January 2021
Published 11 February 2021 Volume 2021:14 Pages 995—1002
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Professor Gaetano Romano
Xiaoling Geng,1 Haoyu Wang,2 Lichen Xu,3 Yazhou Han,3 Yadong Liu3
1Department of Gastroenterology & Hepatology, First Affiliated Hospital of Dalian Medical University, Dalian, 116000, People’s Republic of China; 2Department of Orthopedics, The Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, 710004, Shanxi, People’s Republic of China; 3Department of Orthopedics, Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Dalian, 116000, People’s Republic of China
Correspondence: Yadong Liu
Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Xuegong Street No. 42, Dalian, 116000, People’s Republic of China
Background: MicroRNA-140-5p plays pivotal role in different types of human malignancies, while its involvement in osteosarcoma is unknown.
Objective: Our study aimed to investigate the functionality of microRNA-140-5p in osteosarcoma.
Methods: Plasma levels of microRNA-140-5p and glucose transporter 1 (GLUT-1) in both osteosarcoma and healthy controls were measured by qRT-PCR and ELISA, respectively. Correlation between plasma levels of microRNA-140-5p and GLUT-1 was analyzed by Pearson correlation coefficient. Correlation between plasma levels of microRNA-140-5p and clinical data of patients with osteosarcoma was analyzed by Chi-square test. MicroRNA-140-5p mimic and GLUT-1 expression vector were transfected into cells of human osteosarcoma cell lines, and the effects on microRNA-140-5p expression, GLUT-1 expression and cell proliferation were analyzed by qRT-PCR, Western-blot and CCK-8 assay, respectively.
Results: Plasma levels of microRNA-140-5p were significantly lower and plasma levels of GLUT-1 were significantly higher in osteosarcoma patients than that in healthy controls. Levels of plasma microRNA-140-5p and GLUT-1 were reversely correlated in osteosarcoma patients. Plasma levels of microRNA-140-5p were correlated with tumor size but not with other clinical data of patients. MicroRNA-140-5p mimic significantly inhibited cancer cell proliferation, while GLUT-1 overexpression significantly promoted cancer cell proliferation. MicroRNA-140-5p mimic significantly downregulated GLUT-1 expression.
Conclusion: GLUT-1 overexpression showed no significant effect on microRNA-140-5p expression but attenuated the inhibitory effects of microRNA-140-5p mimic on cell proliferation. We therefore conclude that microRNA-140-5p is downregulated in osteosarcoma and overexpression of microRNA-140-5p may inhibit cancer cell proliferation by downregulating GLUT-1.
Keywords: osteosarcoma, microRNA-140-5p, glucose transporter 1, proliferation
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