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LncRNA DSCAM-AS1 Promotes Colon Cancer Cells Proliferation and Migration via Regulating the miR-204/SOX4 Axis

Authors Lu C, Xie T, Guo X, Wu D, Li S, Li X, Lu Y, Wang X

Received 20 February 2020

Accepted for publication 13 May 2020

Published 9 June 2020 Volume 2020:12 Pages 4347—4356

DOI https://doi.org/10.2147/CMAR.S250670

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Eileen O'Reilly


Canrong Lu,* Tianyu Xie,* Xin Guo, Di Wu, Shuo Li, Xiongguang Li, Yixun Lu, Xinxin Wang

Department of General Surgery, Chinese People’s Liberation Army General Hospital, Beijing 100853, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Xinxin Wang
Department of General Surgery, Chinese People’s Liberation Army General Hospital, Beijing 100853, People’s Republic of China
Email xinxin7374@163.com

Introduction: Long non-coding RNA (lncRNA) DSCAM-AS1 was reported to be aberrantly expressed and play pivotal roles in various human cancers. The aim of the present study was to investigate the expression and roles of DSCAM-AS1 in colon cancer (CC).
Methods: Quantitative real-time PCR (qRT-PCR) was used to detect the expression of DSCAM-AS1, miR-204 and the mRNA level of SOX4. Cell proliferation and cell cycle were analyzed by MTT assay and flow cytometry, respectively. Transwell assay was used for migration capacity detection. Luciferase activity assay was conducted to verify the direct binding of DSCAM-AS1 and miR-204 or miR-204 and SOX4. The protein expression of SOX4 was determined by Western blot. Kaplan–Meier curves were calculated and the Log rank test was performed for the survival data analysis.
Results: DSCAM-AS1 was significantly upregulated in CC and high expression of DSCAM-AS1 was associated with poor prognosis in patients with colon cancer. Knockdown of DSCAM-AS1 significantly suppressed CC cells proliferation and migration. In addition, DSCAM-AS acted as a molecular sponge for miR-204 and SOX4 was identified as a direct target of miR-204 in CC. Moreover, the rescue assay revealed that miR-204 inhibition partly abolished the effects of DSCAM-AS1 knockdown on CC cells proliferation, migration and SOX4 expression.
Discussion: The present study demonstrated that DSCAM-AS1 acted as an oncogenic lncRNA in CC progression by regulating miR-204/SOX4 axis and DSCAM-AS1 may serve as a novel therapeutic target in the treatment of colon cancer.

Keywords: DSCAM-AS1, miR-204/SOX4, colon cancer, proliferation, migration

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