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Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines

Authors Mao Y, Yu Y, Han Y

Received 10 September 2018

Accepted for publication 1 February 2019

Published 20 March 2019 Volume 2019:11 Pages 2253—2259

DOI https://doi.org/10.2147/CMAR.S187019

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 2

Editor who approved publication: Dr Beicheng Sun


Yuqiang Mao,1 Ying Yu,2 Yun Han1

1Department of Thoracic Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, China; 2Liaoning Medical Device Test Institute, Shenyang 110179, China

Background: This study aimed to clarify the effect of thoracic drainage fluid (DF) on lung cancer cells in vitro.
Methods: We assessed the influence of DF on the proliferation and migration of lung cancer cells (LTEP-a-2 and A549) using the MTT cell proliferation assay and scratch wound assay. Cell apoptosis was determined by flow cytometric analysis. We also investigated the effect of DF on drug chemosensitivity, assessing viability of LTEP-a-2 and A549 cells.
Results: The proliferative rates of cancer cells in the DF-treated group were significantly higher than those of the control group. Similar results were obtained for cell migration of lung cancer cells. Cells in the DF-treated groups showed a lower percentage of apoptosis than those of the control groups. Chemosensitivity of lung cancer cells to doxycycline and cisplatin (DDP) was lowered by DF.
Conclusion: These findings suggest that DF affects lung cancer cells by promoting proliferation and migration, inhibiting apoptosis, and increasing drug resistance.

Keywords: lung cancer, drainage fluid, proliferation and migration, apoptosis, drug resistance

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