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Atypical Mature T-Cell Neoplasms: The Relevance of the Role of Flow Cytometry

Authors Statuto T, D'Auria F, Del Vecchio L, Mansueto GR, Villani O, Lalinga AV, Possidente L, Nozza F, Vona G, Rago L, Storto G, Gasparini VR, Zambello R, D'Arena G, Valvano L

Received 28 April 2020

Accepted for publication 23 June 2020

Published 3 August 2020 Volume 2020:13 Pages 7605—7614

DOI https://doi.org/10.2147/OTT.S258512

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Nicola Silvestris

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Teodora Statuto,1,* Fiorella D’Auria,2,* Luigi Del Vecchio3,4,, Giovanna Rosaria Mansueto,5 Oreste Villani,5 Anna Vittoria Lalinga6,, Luciana Possidente,6 Filomena Nozza,1 Gabriella Vona,1 Luciana Rago,7 Giovanni Storto,8 Vanessa Rebecca Gasparini,9 Renato Zambello,10 Giovanni D’Arena,5,* Luciana Valvano1,*

1Laboratory of Clinical Research and Advanced Diagnostics, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 2Unit of Clinical Pathology, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 3CEINGE Biotecnologie Avanzate S.c.a.r.l, Federico II University, Naples, Italy; 4Department of Molecular Medicine and Medical Biotechnology (DMMBM), Federico II University, Naples, Italy; 5Hematology Department of Basilicata, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 6Pathology Unit, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 7Radiotherapy Unit, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 8Department of Nuclear Medicine, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy; 9Department of Medicine, University of Padova - Veneto Institute of Molecular Medicine, VIMM, Padova, PD, Italy; 10Hematology and Clinical Immunology, Department of Medicine, Padua School of Medicine, Padova, PD, Italy

†Anna Vittoria Lalinga passed away on January 26, 2020 and Luigi Del Vecchio passed away on August 16, 2018

*These authors contributed equally to this work

Correspondence: Luciana Valvano
Laboratory of Clinical Research and Advanced Diagnostics, Centro Di Riferimento Oncologico Della Basilicata (IRCCS-CROB), Rionero in Vulture, Pz, Italy
Tel +39 0972 726395
Fax +39 0972 723509
Email [email protected]

Abstract: Lymphoproliferative disorders are a heterogeneous group of malignant clonal proliferations of lymphocytes whose diagnosis remains challenging, despite diagnostic criteria are now well established, due to their heterogeneity in clinical presentation and immunophenotypic profile. Lymphoid T-cell disorders are more rarely seen than B-cell entities and more difficult to diagnose for the absence of a specific immunophenotypic signature. Flow cytometry is a useful tool in diagnosing T-cell lymphoproliferative disorders since it is not only able to better characterize T-cell neoplasms but also to resolve some very complicated cases, in particular those in which a small size population of neoplastic cells is available for the analysis. Here, we report three patients with mature T-cell neoplasms with atypical clinical and biological features in which analysis of peripheral blood and bone marrow specimens by means of multicolor flow cytometry was very useful to identify and characterize three rare T-cell lymphoproliferative disorders, such as angioimmunoblastic T-cell lymphoma, peripheral T-cell lymphoma not otherwise specified and T-cell prolymphocytic leukemia. The aim of this case series report is not only to describe three rare cases of lymphoproliferative neoplasms but also to raise awareness that a fast, highly sensitive, and reproducible procedure, such as flow cytometry immunophenotyping, can have a determinant diagnostic role in these patients.

Keywords: flow cytometry, immunophenotype, angioimmunoblastic T-cell lymphoma, peripheral T-cell lymphoma not otherwise specified, T-cell prolymphocytic leukemia, diagnosis

Introduction

T-cell lymphomas account approximately for 10–15% of lymphoid malignancies.1 According to the most recent World Health Organization (WHO) classification of lymphoid neoplasms, angioimmunoblastic T-cell lymphoma (AITL), peripheral T-cell lymphoma not otherwise specified (PTCL-NOS) and T-cell prolymphocytic leukemia (T-PLL) are categorized as mature T-cell neoplasms.2,3 Common clinical manifestations of T-cell neoplasms include systemic lymphadenopathy, hepatosplenomegaly, fever, anemia, skin rash and serous effusions.

In particular, AITL is a distinct clinical-pathological form of PTCL, accounting for 15–20% of PTCL and 1–2% of all non-Hodgkin lymphoma (NHL) cases and is associated with poor prognosis and a 5-year survival rate of 30–36%.46 The most common and heterogeneous subtype of PTCL is PTCL-NOS, accounting for up to 35% of PTCL cases in Europe and North America. Prognosis is poor, with a 5-year overall survival (OS) of 20–30%.7 T-PLL is an uncommon and aggressive neoplasm, representing 2% of mature lymphoid leukemias. It derives from clonal proliferation of post-thymic T-cells and is associated with a poor prognosis and short survival.8

However, despite diagnostic criteria of these lymphoid matures disorders are now well established, heterogeneity in clinical presentation and immunophenotypic profile often challenges our diagnostic ability. Here we present three cases of mature T-cell neoplasms with clinical and pathological atypical features in which flow cytometry (FCM) allowed a correct diagnosis.

Patients and Methods

Peripheral blood (PB) samples and bone marrow (BM) aspirates from three patients with suspected lymphoproliferative disorders were processed for FCM immunophenotyping using a stain-lyse-no wash technique and a comprehensive seven-color antibody-panel (FITC/PE/PerCP-Cy5-5/PE-Cy7/APC/APC-H7/V500 fluorescent conjugates) on a BD FACSCantoTM II. Our T-cell neoplasms diagnosis panel included monoclonal antibodies against surface (s) and cytoplasmic (cy) antigens: CD3, CD16, CD56, CD45, CD20, CD19, CD8, CD4, CD7, CD2, CD5, TCRαβ, TCRγδ, CD57, CD10, CD34, TdT, HLA-DR and CD1a. Analysis of T-cell clonality was performed using the IOTest Beta Mark TCR Vβ Kit. The B-cell neoplasms diagnostic panel included monoclonal antibodies against CD19, CD5, CD45, CD20, CD23, CD43, CD79b, CD200, FMC7, CD22, sIgkappa, sIglambda. Antibodies were purchased from BD Biosciences (BD), Miltenyi Biotec (MB) or Beckman Coulter (BC). The immunophenotypic analyses were performed using FACSDiva (BD) and Kaluza (BC) Analysis Softwares. To check CD45 negativity in case no. 2, different clones and fluorochromes were tested: 1) BD PerCP Mouse Anti-Human CD45 (2D1 clone), 2) BD V500 Mouse Anti-Human CD45 (HI30 clone), 3) BD APC-H7 Mouse Anti-Human CD45RA (HI100 clone), 4) MB CD45RO-APC human (UCHL1 clone), 5) BC monoclonal CD45-APC-Alexa Fluor 750 (J33 clone). Intracellular staining was performed using the BD IntraSure Kit, according to the manufacturer’s protocol. For CD3, two different clones and fluorochromes were used: CD3 FITC (SK7 clone, BD) and CD3 APC (UCHT1 clone, BD). In our assays, we included viable cells in the analysis and excluded any debris, dead cells and clumps or doublets using forward and side scatter (very low) parameters. The pathological populations were established based on the evidence of monoclonality or of an aberrant B or T immunophenotype. The quantitative determination of the TCR-Vβ repertoire of human T lymphocytes was assessed using IOTest® Beta Mark TCR V Kit (BC), including CD3-FITC and CD45-PerCP in each tube for gating aberrant T-cell populations. The FC TCR-Vβ results were considered to be positive for clonality if: (a) a single TCR-Vβ was expressed by at least 50% of a gated T cell subset; or (b) a TCR-Vβ was expressed at a frequency 10 times above its normal limit, based on the manufacturer’s reported ranges for total T cells or T cell subsets; or (c) at least 70% of gated T cells failed to react to any of the TCR-Vβ antibodies, presumably due to expression of a TCR-Vβ not recognized by the antibody panel. The last set of cases was defined as “non-reactive”.9 Morphological features were evaluated by hematoxylin and eosin (H&E)-stained BM aspirates, lymph nodes (LNs) and sections of the skin lesions. Fluorescent In Situ Hybridization (FISH) was performed according to the instructions of the manufacturer on fixed nuclei by using the commercially available panel Vysis CLL FISH Probe Kit (Abbott Molecular) to detect deletion of the LSI TP53, LSI ATM and LSI D13S319 probe targets. A selective Polymerase chain reaction (PCR) was used to analyze exon 16 of STAT5b and exon 21 of STAT3, as previously reported.1012 The analysis of the immunoglobulin heavy variable (IGHV) sequences was performed by Next Generation Sequencing (NGS) technique, carried out according to the IMGT-V-Quest, Arrest/AssignSubsets criteria.13

Clinical and laboratory features, immunohistochemical results at diagnosis and FCM immunophenotyping data at diagnosis on BM aspirates of the three patients are summarized in Tables 1, 2 and 3

Table 1 Clinical and Laboratory Features of the Three Cases at Diagnosis

Table 2 Immunohistochemical Results at Diagnosis

Table 3 FC Immunophenotype Results at Diagnosis on BMA Samples

Case 1

A 77-year-old man with multiple enlarged axillary and inguinal LNs was admitted at our Hospital in March 2016. Despite a normal leukocyte count, without atypical morphological findings, a routine FCM immunophenotyping analysis of PB lymphocytes revealed the presence of a small (2% of CD45+ cells) and abnormal population of CD4+ CD8 T-cells with decreased CD3 expression (CD3+ dim) (Figure 1AD). This population also showed the positive expression of the T-cells markers CD7, CD2 and CD5 and the negative expression for the 24 rearrangements of TCRVβ repertoire tested. Furthermore, this T-cell population also expressed CD10 (Figure 1E). A biopsy of a left axillary LN established the diagnosis of AITL. The LN displayed a prominent proliferation of high endothelial venules, an increase of dendritic cells and a diffuse infiltration of a mixed population of eosinophils, neutrophils, plasma cells, with medium- to small-sized atypical lymphoid cells (Figure 1F) positive for CD3, CD4, CD5 (Figure 1G), CD10 (Figure 1H) and CD7 (focal), and negative for CD20 (Figure 1I), CD79a and CD30. Proliferative index, assessed by Ki67 staining, was 65–70%. Abnormal cells were mixed to several reactive CD8+ T lymphocytes. Whole-body positron emission computed tomography (PET-CT) scan evidenced generalized pathological lymphadenopathy above and below the diaphragm (highest SUV: 5.4).

Figure 1 Case 1. Before histological diagnosis, flow cytometry analysis detected an aberrant T-cell population in peripheral blood (PB) (shown in blue). The aberrant T-cells were identified in the area of lymphocytes by using the Forward versus Side Scatter (FSC vs SSC) gating (panel A) and CD45 versus SSC (panel B) gating. These cells were positive for CD3 (dim) and CD4 (C) and negative for CD8 (D). Of note, CD10 was expressed by these cells (E). Lymph node biopsy showed typical morphological (hematoxylin and eosin, H&E) ((F); original magnification ×20) and immunohistochemical findings with positivity for CD5 ((G); original magnification ×10), CD10 ((H); original magnification ×20) and negativity for CD20 ((I); original magnification ×10). Bone marrow biopsy at diagnosis (J) showed negativity for CD3 ((K); original magnification ×20), CD4 ((L); original magnification ×20) and CD20 ((M); original magnification ×20).

In April 2016, FCM analysis performed on both BM and PB confirmed the presence of CD3+dim CD4+CD5+CD8CD10+ T-cells accounting for 3% of CD45+ cells in PB and 0.3% of all nucleated cells in BM. Notably, morphologic evaluation of BM smears and bone marrow biopsy (BMB) was not able to detect any BM infiltration (Figure 1JM). From April 2016 to September 2016, the patient underwent chemotherapy with COMP (cyclophosphamide, vincristine, liposomal doxorubicin and prednisone) regimen for a total of six courses. In September 2016, PET-CT scan was negative, and the patient was considered in complete remission. In February 2017, the patient was still in good general conditions, with a completely negative total body CT scan. However, 6 months later (July 2017), the patient developed paresthesia of the hands and PET-CT scan showed some areas of doubtful abnormal uptake, including axillary and mediastinal LNs (SUV up to 2.4). In November 2017, the patient reported high fever and CT scan showed a clear progressive disease at multiple sites, including bilateral LNs of cervical, intra-retroperitoneal and inguinal regions, axillary fossa and mediastinum. Splenomegaly was also detected. FCM analysis performed on BM to monitor AITL cells, confirmed the presence of a very small pathological T-cell population (0.36% of all nucleated cells) with the same immunophenotype seen at diagnosis, while FSC (Forward Scatter), SSC (Side Scatter) and CD45 FC parameters did not apparently detect abnormal CD3 negative lymphoid cells, so that a more extensive study of B cell markers (CD20, CD19, sIg kappa, sIg lambda) was not performed. Unexpectedly, BMB showed a diffuse, interstitial infiltration of “immunoblastic-like” lymphoid cells (Figure 2A), negative for CD5 (Figure 2B), but positive for CD20 (Figure 2C), CD30 (Figure 2D) and CD79a, mixed to several small reactive T lymphocytes and rare mature plasma cells. The conclusion was a diagnosis of DLBCL. The LN biopsy of the initial AITL-diagnosis and the BMB involved by DLBCL were tested by immunohistochemistry for Epstein-Barr Virus (EBV) (by using DAKO Monoclonal Mouse Anti-Epstein-Barr Virus, LMP, CS.1–4 clone): both samples resulted positive (Figure 2E and F). The patient died in December 2017 for a progressive multiorgan failure.

Figure 2 Case 1. Bone marrow (BM) biopsy at progression showing morphological (hematoxylin and eosin, H&E) lymphoid cells (A); original magnification ×20) negative for CD5 (B); original magnification ×20) and positive for CD20 (C); original magnification ×20), CD30 (D); original magnification ×20). Immunohistochemical positivity for EBV (by using Monoclonal Mouse Anti-Epstein-Barr Virus, LMP) of diffuse large B-cell lymphoma in lymph node biopsy ((E); original magnification ×20) and BM aspirate ((F); original magnification ×10).

Case 2

In May 2018 a 60-year-old man with a history of diabetes mellitus, arterial hypertension, and skin lesions on head and inguinal regions treated for 8 months with clobetasol was admitted at our hospital because of the appearance of new skin lesions. PET-CT scan (Figure 3A) demonstrated a faint hyperaccumulation of the tracer in correspondence of the voluminous subcutaneous thickenings in the left parietal, right dorsal and homolateral lumbar regions and an absence of uptake in a nodulariform thickening in the subcutaneous tissue of the right scapular region and in the numerous LNs in bilateral inguinal and axillary regions and in Barety’s space. Physical examination showed cutaneous plaques on the scalp (3 cm, purple with a central encrusted region), on the right gluteus (8x8 cm, red-violet), on the right shoulder blade (5 x 8 cm, pink) and in the right inguinal region (3 x 2.5 cm, reddish), splenomegaly and LN enlargements. Cutaneous biopsies of backside and scalp lesions showed infiltration, diffuse and subtotal, of small and medium-sized lymphoid elements, with pleomorphic nuclei, extended to the superficial and deep reticular dermis, in the absence of epidermotropism (Figure 3B). These cells were found to be CD3+, CD4+, CD5+, CD8 (Figure 3CF), CD56, CD7, CD57, CD30, CD20 with Ki67 cell proliferation index about 20%. In addition, some small B-cell lymphocytes (CD20+) were interspersed. The conclusive diagnosis was PTCL-NOS. BM examination did not show a cytologically relevant lymphomatous infiltration while FC immunophenotyping (Figure 3GK) documented a CD3+ T-cell population (13%) more than half (51.5%) of which were abnormal T-cells displaying the following phenotype: CD4+, CD2+, CD7+dim, CD5+, TCRαβ+, CD45, CD45RA, CD45RO, CD8, CD57, CD10, CD16, CD56, TdT, TCRγδ and “non-reactive” for the 24 rearrangements of TCRVβ tested. The patient received six cycles of CHOEP (Cyclophosphamide–Doxorubicin–Vincristine–Etoposide–Prednisone) chemotherapy regimen. Afterwards, the patient underwent radiation treatment on constant PET localizations. The last FCM follow-up performed on BM (in May 2019) showed a minimal residual disease (MRD) of 3.1% on all nucleated cells. Currently, the patient had a relapse.

Figure 3 Case 2. PET-CT scan (A) showed voluminous subcutaneous thickenings in dorsal and lumbar regions (circled areas). Hematoxylin and eosin (H&E) (B); original magnification ×20) on the biopsies of backside and scalp cutaneous lesions showed infiltration of small and medium-sized lymphoid elements, positive for CD3, CD4, CD5 (CE), original magnification ×10) and negative for CD8 (F), original magnification ×10). FCM immunophenotyping on bone marrow aspirate (GK) showed pathological T cells positive for CD3, CD4, CD7 (dim) and CD5 (in green color), and negative for CD45, CD8 and TdT.

Case 3

A 79-year-old male was admitted at our Hospital in June 2018, due to an increased number of circulating white blood cells (WBC) (38 x 103/µL; lymphocytes 84%, neutrophils 16%). The patient had a previous history of ischemic heart disease and presented with bilateral neck, axillary and inguinal small (<1–2 cm) superficial LNs. BM aspirate showed a widespread lymphoid infiltration, predominantly small “mature” lymphocytes with very rare nucleoli, representing about half of the total cells (Figure 4A and B). CT scan also showed multiple abdominal LNs. FCM immunophenotyping of both PB and BM aspirates showed the presence (50% and 60%, respectively, on all nucleated cells) of sCD3-, cyCD3+, CD8-, CD2+, CD7+, CD5+, CD4+, CD56-, CD57-, CD16-, HLA-DR-, CD34-, CD1a-, TdT-, CD10-, TCRαβ- and TCRγδ- pathological T cells; the 75% of these cells expressed CD45 on their membrane. Notably, these T-cells were mixed with a small population of clonal B lymphocytes (3,1% of leukocytes in PB, 2.2% of all nucleated cells in BM), that expressed CD19, CD5, CD20 (dim), CD23, CD43, CD200, sIgkappa (dim), with a D’Arena Score of 4 (Figure 4CH).14 Trephine BM biopsy showed two nodular aggregates of small and sometimes medium-sized lymphoid elements with predominantly T phenotype (CD57-, CD8-, CD5+, CD3+, CD4+, CD56-), mixed with rare CD20+, CD79α+ small B lymphocytes (Figure 4IQ). FISH analysis, performed on unpurified nuclei, documented ATM and 13q14.3 deletions in 50% of cells, while 36% only displayed ATM deletion. A second FISH performed on purified CD19+ cells showed only the deletion of chromosome 13q14.3. IGHV sequences showed an unmutated status. Molecular analysis highlighted no mutations for both the exon 16 of the STAT5b gene and the exon 21 of the STAT3 gene. A final diagnosis of T-PLL (likely “small cell variant”, according to WHO classification)3 with concomitant Monoclonal B-cell lymphocytosis (MBL) was made. Despite the treatment with bendamustine and CHOP (cyclophosphamide, vincristine, doxorubicin and prednisone) regimen the patient showed a progressive increase of WBC up to 500 x 103/µL, with an increase of pathological T population (90% of total nucleated cells) and disappearance of the clonal MBL B-cells. The patient died during pentostatin treatment.

Figure 4 Case 3. Bone marrow (BM) aspirate showed a widespread lymphoid infiltration, predominantly small “mature” lymphocytes with very rare nucleoli (A and B). Examples of immunophenotypic features seen on peripheral blood samples of the reported case of concomitant T-PLL and MBL. Neoplastic T cells are in purple and express CD4 (D and lack CD8 (E) and CD34 (F). They lack surface CD3 expression, but present cytoplasmic CD3 (G). CD45 was expressed by only a subset of pathological T lymphocytes (C). B-CCL/SLL cells (in yellow) on the PB sample present typical co-expression of CD19 and CD5 (H). Trephine BM biopsy of the reported case of concomitant T-PLL and MBL displayed small and sometimes medium-size lymphoid elements (Q), original magnification ×20) with predominantly T phenotype: CD3+ (I), original magnification ×20), CD4+ (J), original magnification ×20), CD5+ (K), original magnification ×20), CD8- (L), original magnification ×20), CD20- (M), original magnification ×20), CD56- (N), original magnification ×20), CD79- (O), original magnification ×10) and CD57- ((P), original magnification ×20).

Discussion

Multiparameter FCM is a fast, highly sensitive, and reproducible procedure to evaluate the immunophenotype of T lymphocytes,15 allowing the identification and characterization of T-cell neoplasms. Among them, AITL, PTCL-NOS and T-PLL must be considered despite very rarely seen. Typical immunophenotype of AITL is CD3+, CD4+, CD8 and, frequently CD10+. However, several authors believe that the only presence of a small number of CD4+CD10+ T-cells, in the absence of other phenotypic aberrancies, should not be considered a sign of the presence of neoplastic cells and correlation with all available morphologic and immunophenotypic data is necessary to make a definitive diagnosis.1619 In this setting, Loghavi et al20 reported a case of AITL involving LNs and PB without CD10 expression on T-cells. We have described here an unusual case of CD3+ dim CD4+ CD8 CD5+ CD10+ AITL, with a limited infiltration of BM, detected by FCM on BM (and PB), but not by BMB, using conventional IHC. Another interesting aspect of this case was the sequential development of DLBCL after the treatment of AITL with COMP regimen.3 DLBCL is the most frequent type of NHL, representing about 30–40% of all cases21 and cases of DLBCL arising after the initial diagnosis of AITL have been already reported.26 Interestingly, however, very few EBV positive cases are described in the literature2224 and both LN biopsy of the initial AITL-diagnosis and the BMB involved by DLBCL resulted positive for EBV, as assessed by IHC. PTCL-NOS is characterized by an aberrant T-cell phenotype, with frequent loss of CD5 and CD7. A CD4+/CD8 phenotype predominates in nodal cases. CD4+/CD8+ or CD4−/CD8 phenotypes are sometimes seen as CD8, CD56 and cytotoxic granule expression.25 An analysis of prognostic factors in a group of 82 patients found that a favorable outcome was associated with a CD3+CD4+CD8 phenotype and with localized skin lesions.26 PTCL-NOS usually lacks CD10 expression and proliferation rate is usually high with Ki-67 exceeding 80% associated with a worse prognosis.27 The expression of CD30 has been reported in about 32–58% of PTCL-NOS cases with variable prognosis.28,29

CD45 is expressed on nucleated hematopoietic cells in healthy individuals with variable intensity. According to the cell type, the two major human isoforms (CD45RA and CD45RO) are expressed differently on T-cells.3033 Different hematopoietic neoplasms with the loss of CD45 surface expression have been reported in the literature.30,34-37

The European Group for the Immunological Characterization of Leukemias (EGIL) has established guidelines for the characterization of acute leukemias based on marker expression. T lineage acute lymphoblastic leukemia (ALL) is defined by the cytoplasmic or membrane expression of CD3. Expression of the T-cell antigens CD2 and CD7 is not sufficient to define a case as T-ALL. Four subgroups from pro-T-ALL (or T-I) to mature T-ALL (or T-IV) have been also described according to the degree of thymic differentiation. Within T-ALL, two subgroups were defined according to the membrane expression of TCR α/β chains and TCR γ/δ in association with CD3. Mature-T-ALL is defined by the expression of membrane CD3 and the negative expression of CD1a. Most cases of T-ALL are TdT+, HLA-DR, CD34, but these markers are not considered for diagnosis or disease classification. The expression of TdT is considered important for the diagnosis of ALL, to distinguish them from mature lymphoid malignancies; however, TdT as well as other hematopoietic cell precursor associated markers, such as CD34 and class II HLA-DR, do not account for disease classification purposes.38 In the current case, pathological T-cells expressed CD3, but were negative for CD45. The expression of TCRαβ, as surface transmembrane receptor, the lack of TdT and morphological (predominantly small “mature” lymphocytes with very rare nucleoli and two nodular aggregates of small and sometimes medium-sized lymphoid elements) and histological analyses excluded the possibility that pathological cells could be leukemic blasts, confirming infiltration of lymphoid elements with T phenotype. The “non-reactive” TCR Vβ FCM phenotype further demonstrated the presence of a pathological population, characterized by the loss of CD45. T-PLL is a rare T-cell neoplasm with a post-thymic (TdT, CD1a), mature (CD2+, CD5+, CD7+, CD16 and CD56) T-cell immunophenotype, with variable CD4 and CD8 expression (generally CD4+/CD8). It is mostly a CD45+ cyCD3+ T-cell leukemia, but in 5% to 10% of cases, either of these markers may be negative, while simultaneous loss of both is extremely rare.39 CD3 is an important T cell marker. Its presence at all stages of T cell development makes it an ideal T cell marker for both the detection of normal and neoplastic T cells and a useful immunohistochemical marker for T cells in tissue sections. CD3 can be found both on the cell membrane and/or in the cytoplasm of T cell neoplasms.40

In the current case, pathological T-cells were negative for surface CD3 and only the 76% of them expressed the CD45 antigen. Furthermore, they were also accompanied by a small population of B lymphocytes with chronic lymphocytic leukemia (CLL) immunophenotype.

MBL is defined as the presence of a clonal B-cell population in the peripheral blood with fewer than 5 × 109/L B-cells and no other signs of a lymphoproliferative disorder (eg, lymphadenopathy, organomegaly, cytopenia, or extramedullary involvement).41 The majority of cases of MBL have the immunophenotype of CLL.42 The use of alemtuzumab (anti-CD52 antibody) in symptomatic T-PLL patients has improved OS over the traditional alkylating agents or purine analog-based chemotherapy. For patients who fail to attain a complete remission with alemtuzumab, the addition of pentostatin should be considered.43 A retrospective study in 15 patients showed that bendamustine is a valuable treatment option for T-PLL, confirming the good overall response rate (ORR) of single-agent bendamustine in mature T cell neoplasms.44 In the present case, considering the ischemic heart disease of the patient and the cardiotoxic side effects of alemtuzumab,45 the patient initially received three cycles of bendamustine. Cases no. 2 and 3 demonstrated the FCM ability to analyze multiple markers simultaneously on populations with anomalous immunophenotype (eg, the lack of CD45 and CD3 on surface), which, if not evidenced, could lead to an incorrect immunophenotypic diagnosis and monitoring of neoplasm. Our data confirm that there are situations where FCM may play a determinant role, providing objective and quantitative results, even on very small populations within few hours. Furthermore, this advanced technique results in superior to IHC in the cases where the pathological cellular population is characterized by the concurrent expression of multiple aberrant antigens. Currently, the high sensitivity and the multiparametric feature of FCM analysis make it a useful tool for diagnosis and residual disease monitoring of T-cell neoplasms.

Informed Consent

Written informed consents were obtained from the patients for their anonymized data to be published in this article.

Ethics Approval

Ethical approval to report these cases was obtained from Comitato Etico Unico Regionale per la Basilicata (20140040750).

Acknowledgments

We would like to thank Dr. Luigi Del Vecchio and Dr. Anna Vittoria Lalinga, who, although no longer with us, continue to inspire us with the example and the dedication demonstrated over the course of their careers.

Author Contributions

All authors contributed to data analysis, drafting or revising the article, gave final approval of the version to be published, and agree to be accountable for all aspects of the work.

Disclosure

The authors declare that there are no conflicts of interest.

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