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A Thinkful of “Alginate Beads as a Promising Tool for Successful Production of Viable and Pluripotent Human-Induced Pluripotent Stem Cells in a 3D Culture System” [Letter]

Authors Rilianawati, Rinendyaputri R , Purwaningtyas YR 

Received 19 December 2023

Accepted for publication 8 January 2024

Published 16 January 2024 Volume 2024:17 Pages 1—2


Checked for plagiarism Yes

Editor who approved publication: Dr Bernard Binetruy

Rilianawati,1 Ratih Rinendyaputri,2,3 Yoggi Ramadhani Purwaningtyas2

1Vaccine and Drug Research Center, National Research and Innovation Agency, Serpong, Indonesia; 2Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency, Bogor, Indonesia; 3Graduate School of Biomedical, Sciences, Doctoral Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia

Correspondence: Rilianawati, Email [email protected]

View the original paper by Dr Alsobaie and colleagues

Dear editor

We have read the published article, and we appreciate the authors who have reported the article about “Alginate Beads as a Promising Tool for Successful Production of Viable and Pluripotent Human-Induced Pluripotent Stem Cells in a 3D Culture System”, published in Stem Cells and Cloning: Advances and Applications s 2023:16 61–73. We appreciate the success of Alsubaie et al in carrying out 3D culture using the encapsulation technique with alginate and gelatin on induced pluripotent cell/iPSCs so that they can suppress their teratoma properties.1

The encapsulation process is carried out on stem cells such as induced pluripotent cell/iPSC and mesenchymal stem cell/MSC to make them more stable, resistant to temperature and maintain their viability.1,2 Previous research has shown the antibacterial hydrogel was able to kill approximately 99% of the exposed bacteria after 3 h of exposure. The developed antibacterial hydrogels are light weight, have a high water-uptake capacity, and show high biocompatibility with the model mammalian cells, which make them a promising candidate to be used for wound dressing applications.3

This study directly Human IPSCs under dynamic 3D culture were able to secrete the necessary ECM components to form a suitable niche, which might help to maintain the pluripotent state. Meanwhile, in our opinion, it is also necessary to mention Dynamic 3D culture is desirable for the large-scale expansion of undifferentiated human IPSCs for what therapy or research expansion applications. We suggest using encapsulated herbs such as lemongrass and various herbs to protect cell DNA when large-scale expansion is carried out.4


The authors report no conflicts of interest in this communication.


1. Alsobaie S, Alsobaie T, Alshammary AF, Abudawood A, Mantalaris A. Alginate beads as a promising tool for successful production of viable and pluripotent human-induced pluripotent stem cells in a 3D culture system. Stem Cells Cloning. 2023;16:61–73. doi:10.2147/SCCAA.S409139

2. Harlim A, Rilianawati E. Optimization of alginate-based encapsulation utilization for viability and stability of the mesenchymal stem cell. J Stem Cell Res Ther. 2018;8:412. doi:10.4172/2157-7633.1000412

3. Tavakolian M, Munguia-Lopez JG, Valiei A, et al. Highly absorbent antibacterial and biofilm-disrupting hydrogels from cellulose for wound dressing applications. ACS Appl Mater Interfaces. 2020;12:39991–40001. doi:10.1021/acsami.0c08784

4. Khorshidian N, Mahboubi A, Kalantari N, et al. Chitosan-coated alginate microcapsules loaded with herbal galactagogue extract: formulation optimization and characterization. Iran J Pharm Res. 2019;18(3):1180–1195. doi:10.22037/ijpr.2019.1100776

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