Back to Journals » Infection and Drug Resistance » Volume 13

Whole-Genome Analysis of Two Copies of blaNDM-1 Gene Carrying Acinetobacter johnsonii Strain Acsw19 Isolated from Sichuan, China

Authors Tang L, Shen W, Zhang Z, Zhang J, Wang G, Xiang L, She J, Hu X, Zou G, Zhu B, Zhou Y

Received 26 October 2019

Accepted for publication 3 March 2020

Published 23 March 2020 Volume 2020:13 Pages 855—865

DOI https://doi.org/10.2147/IDR.S236200

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Joachim Wink


Lingtong Tang, 1, 2 Wei Shen, 2, 3 Zhikun Zhang, 2 Jingping Zhang, 2 Guangxi Wang, 2 Li Xiang, 2 Junping She, 2 Xiaoyan Hu, 2 Guoyuan Zou, 4 Baoli Zhu, 5 Yingshun Zhou 2

1Department of Clinical Laboratory, The People’s Hospital of Gao County, Sichuan 644000, People’s Republic of China; 2Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, Luzhou, Sichuan, People’s Republic of China; 3Department of Clinical Laboratory, The First People’s Hospital of Yibin, Yibin 644000, Sichuan, People’s Republic of China; 4Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Science, Beijing, People’s Republic of China; 5Key Laboratory of Pathogenic Microbiology & Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People’s Republic of China

Correspondence: Yingshun Zhou
Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, No. 319, Zhongshan Road, Luzhou 646000, Sichuan, People’s Republic of China
Tel +86– 0830– 3160073
Email yingshunzhou@swmu.edu.cn

Guoyuan Zou
Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Science, Beijing, People’s Republic of China
Email gyzou@163.com

Purpose: To characterize the genetic feature of the carbapenems resistant Acinetobacter johnsonii strain Acsw19 isolated from municipal sludge. This strain was found to carry two copies of blaNDM-1, cmlB1-like gene, and blaOXA-211-like gene along with other 8 antimicrobial resistance genes, 3 plasmids, 15 genomic islands and 8 prophages.
Methods: A carbapenem-resistant Acinetobacter johnsonii strain Acsw19 isolated from municipal sludge was subjected to whole-genome sequencing (WGS) via the PacBio and Illumina MiSeq platforms. Thereafter, the characteristic was analyzed by a series of bioinformatics software.
Results: The results showed that the genome of Acsw19 was consisted of a 3,433,749 bp circular chromosome and 3 circular plasmids, pAcsw19-1 (11,161 bp), pAcsw19-2 (351,885 bp) and pAcsw19-3 (38,391bp), respectively. Resistome analysis showed that Acsw19 carried 12 antimicrobial resistance genes, including 6 [cmlB1-like, blaNDM-1, blaOXA-58, aph ()-VIa, msr(E) and mph(E)] in the plasmid pAcsw19-2 and 6 (blaOXA-211-like, blaNDM-1, aph(3")-Ib, aph(6)-Id, sul2, and floR) in the chromosome genome. Specifically, the cmlB1-like gene shared 86.33%, 71.7% and 71.9% similarities with the cmlB1, cmlA4 and cmlA8 gene, and the blaOXA-211-like gene shared 94.4%, 95.39% and 96.36% similarities with blaOXA-211, blaOXA-643 and blaOXA-652, at the nucleotide level, respectively. Phylogenetic analysis showed that the blaOXA-211-like gene and cmlB1-like gene had the closest evolutionary relationship with blaOXA-643 and cmlB1, respectively. These results indicated that the blaOXA-211-like and cmlB1-like genes identified in the current study should be the novel variant resistance genes.
Conclusion: Carrying of two copies of blaNDM-1, cmlB1-like, blaOXA-211-like and along with other 8 antimicrobial resistance genes, 3 plasmids, 15 genomic islands and 8 prophages Acinetobacter johnsonii strain might increase the possibility of spreading of resistance genes.

Keywords: Acinetobacter johnsonii, blaNDM-1, blaOXA, genomic island

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]