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USP21 promotes cell proliferation and metastasis through suppressing EZH2 ubiquitination in bladder carcinoma

Authors Chen Y, Zhou B, Chen D

Received 16 October 2016

Accepted for publication 23 November 2016

Published 7 February 2017 Volume 2017:10 Pages 681—689

DOI https://doi.org/10.2147/OTT.S124795

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 3

Editor who approved publication: Dr Tohru Yamada

Yong Chen,1 Bo Zhou,2 Daihui Chen1

1Department of Urology, The First Affiliated Hospital of Chongqing Medical University, 2Department of Urology, Daping Hospital, Third Military Medical University, Chongqing, People’s Republic of China

Abstract: Bladder cancer (BC) is the second most common malignant tumor of the urinary tract in the world. In this study, we found that ubiquitin-specific protease (USP21) was upregulated in BC and the ectopic expression of USP21 was closely associated with tumor size and metastasis. Moreover, patients with higher levels of USP21 had poorer survival rate. Multiple function analysis such as CCK-8, colony formation, wound healing, and transwell analysis indicated that USP21 regulated cell proliferation and metastasis in bladder carcinoma cell lines. We also found that USP21 could facilitate epithelial–mesenchymal transition. As EZH2 has been reported to promote cell metastasis in BC, our work identified that USP21 deubiquitinated EZH2 and stabilized it. Our data demonstrated that USP21 might play a crucial role in regulating BC progression and could provide a potential therapeutic strategy for BC.

Keywords: USP21, proliferation, metastasis, EZH2, EMT

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