Back to Journals » OncoTargets and Therapy » Volume 11

The functional verification of EGFR-CAR T-cells targeted to hypopharyngeal squamous cell carcinoma

Authors Dong Y, Ding Y, Guo W, Huang J, Yang Z, Zhang Y, Chen X

Received 28 May 2018

Accepted for publication 20 August 2018

Published 16 October 2018 Volume 2018:11 Pages 7053—7059


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Federico Perche

Yi-Han Dong,1 Yi-Ming Ding,2 Wei Guo,2 Jun-Wei Huang,2 Zheng Yang,2 Yang Zhang,2 Xiao-Hong Chen2

1Department of Otolaryngology, Daqing Oilfield General Hospital, Daqing City 163001, Heilongjiang Province, People’s Republic of China; 2Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, People’s Republic of China

The aim of this study was to validate the antitumor function of EGFR-chimeric antigen T-cells (CART) targeted to FaDu cells, a hypopharyngeal squamous cell carcinoma cell line, and to provide a preclinical basis for the application of CART cell technology in hypopharyngeal squamous cell carcinoma.
Methods: Detection of cytokine secretions of EGFR-CAR T and CART-controls in the presence of target cells and nontarget cells as an indicator of CART cell activation. Detection of the cytotoxic effects of EGFR-CAR T on specific tumors in the presence of target cells was evaluated by LDH release and CART cell proliferation.
Results: The results showed that cytokine secretion increased significantly after EGFR-CAR T-cells were incubated with target cells, and EGFR-CAR T-cells has higher cytotoxic effect on target cells than the CART-control group. The target cell lysis rate was 52.66%. The proliferation of EGFR-CAR T-cells in the presence of target cells was not distinctly observed.
Conclusion: In this study, we validated the antitumor function of EGFR-CAR T-cells targeted to the FaDu cell line and provided the foundation for application of the CART technique in the treatment of hypopharyngeal carcinoma.

Keywords: chimeric antigen receptor T-cells, epidermal growth factor receptor, hypopharyngeal neoplasm

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]