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The functional verification of EGFR-CAR T-cells targeted to hypopharyngeal squamous cell carcinoma

Authors Dong Y, Ding Y, Guo W, Huang J, Yang Z, Zhang Y, Chen X

Received 28 May 2018

Accepted for publication 20 August 2018

Published 16 October 2018 Volume 2018:11 Pages 7053—7059

DOI https://doi.org/10.2147/OTT.S175516

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 2

Editor who approved publication: Dr Federico Perche


Yi-Han Dong,1 Yi-Ming Ding,2 Wei Guo,2 Jun-Wei Huang,2 Zheng Yang,2 Yang Zhang,2 Xiao-Hong Chen2

1Department of Otolaryngology, Daqing Oilfield General Hospital, Daqing City 163001, Heilongjiang Province, People’s Republic of China; 2Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, Beijing, People’s Republic of China

Background:
The aim of this study was to validate the antitumor function of EGFR-chimeric antigen T-cells (CART) targeted to FaDu cells, a hypopharyngeal squamous cell carcinoma cell line, and to provide a preclinical basis for the application of CART cell technology in hypopharyngeal squamous cell carcinoma.
Methods: Detection of cytokine secretions of EGFR-CAR T and CART-controls in the presence of target cells and nontarget cells as an indicator of CART cell activation. Detection of the cytotoxic effects of EGFR-CAR T on specific tumors in the presence of target cells was evaluated by LDH release and CART cell proliferation.
Results: The results showed that cytokine secretion increased significantly after EGFR-CAR T-cells were incubated with target cells, and EGFR-CAR T-cells has higher cytotoxic effect on target cells than the CART-control group. The target cell lysis rate was 52.66%. The proliferation of EGFR-CAR T-cells in the presence of target cells was not distinctly observed.
Conclusion: In this study, we validated the antitumor function of EGFR-CAR T-cells targeted to the FaDu cell line and provided the foundation for application of the CART technique in the treatment of hypopharyngeal carcinoma.

Keywords: chimeric antigen receptor T-cells, epidermal growth factor receptor, hypopharyngeal neoplasm

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