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The expression of microRNA 146a in patients with ischemic stroke: an observational study

Authors Kotb HG, Ibrahim AH, Mohamed EF, Ali OM, Hassanein N, Badawy D, Abdelatty Aly E

Received 26 April 2019

Accepted for publication 18 July 2019

Published 9 August 2019 Volume 2019:12 Pages 273—278

DOI https://doi.org/10.2147/IJGM.S213535

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Melinda Thomas

Peer reviewer comments 3

Editor who approved publication: Dr Scott Fraser


Hend G Kotb,1 Amal H Ibrahim,1 Eman F Mohamed,1 Omaima M Ali,2 Nagwa Hassanein,3 Dina Badawy,3 Ehab Abdelatty Aly4

1Department of Internal Medicine, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 2Department of Internal Medicine, Faculty of Medicine, Aswan University, Aswan, Egypt; 3Department of Clinical Pathology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt; 4Health Radiation Research Department, National Center of Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt

Aim: We conducted the present prospective study to assess the level of microRNA (miRNA) 146a in patients with ischemic stroke and its correlation with patients’ characteristics.
Methods: We conducted an observational study that included adult patients (≥18 years old) who presented within 24 hrs after the onset of the symptoms of acute ischemic stroke. In addition, age- and sex-matched healthy volunteers were included as control group. The primary outcome in the present study was the difference in miRNA 146a expression between patients with ischemic stroke and control group participants. The expression of miRNA 146a was measured using quantitativereal-time PCR. Quantitativereal-time PCR amplification and analysis were performed using Rotor-Gene Q thermal cycler.
Results: The present study included 44 patients with ischemic stroke and 22 matched controls. Regarding the primary outcome of the present study, the median expression of miRNA 146a in patients with ischemic stroke was −1.98 fold (IQR −27.1–3.9) compared to 1.75 fold (IQR −2.25–5.27) in control group (P<0.001). However, the subgroup analysis showed that the expression of miRNA 146a was significantly downregulated in comatosed patients only (P<0.001). The expression of miRNA 146a correlated negatively with Glasgow Coma Scale score in comatose patients (r=−0.352, P=0.022).
Conclusion: In conclusion, the expression of miRNA 146a is significantly downregulated in ischemic stroke patients. Further studies are needed to assess its diagnostic utility and therapeutic potentials.

Keywords: ischemic stroke, coma, cerebral infarction, miRNA 146a


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