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The Antibacterial Mechanism of Silver Nanoparticles and Its Application in Dentistry

Authors Yin IX , Zhang J, Zhao IS , Mei ML , Li Q, Chu CH 

Received 21 January 2020

Accepted for publication 29 March 2020

Published 17 April 2020 Volume 2020:15 Pages 2555—2562


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 4

Editor who approved publication: Prof. Dr. Anderson Oliveira Lobo

Iris Xiaoxue Yin,1,2 Jing Zhang,3 Irene Shuping Zhao,1 May Lei Mei,4 Quanli Li,3 Chun Hung Chu2

1School of Dentistry, Shenzhen University Health Science Center, Shenzhen, People’s Republic of China; 2Faculty of Dentistry, The University of Hong Kong, Hong Kong; 3College of Stomatology, Anhui Medical University, Hefei, People’s Republic of China; 4Faculty of Dentistry, University of Otago, Otago, New Zealand

Correspondence: Irene Shuping Zhao
School of Dentistry, Shenzhen University Health Science Center, Shenzhen, People’s Republic of China
Tel +86 18823781964
Email [email protected]
Chun Hung Chu
Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, People’s Republic of China
Tel +852 2859 0287
Fax +852 2858 2532
, Email [email protected]

Abstract: Nanotechnology has recently emerged as a rapidly growing field with numerous biomedical science applications. At the same time, silver has been adopted as an antimicrobial material and disinfectant that is relatively free of adverse effects. Silver nanoparticles possess a broad spectrum of antibacterial, antifungal and antiviral properties. Silver nanoparticles have the ability to penetrate bacterial cell walls, changing the structure of cell membranes and even resulting in cell death. Their efficacy is due not only to their nanoscale size but also to their large ratio of surface area to volume. They can increase the permeability of cell membranes, produce reactive oxygen species, and interrupt replication of deoxyribonucleic acid by releasing silver ions. Researchers have studied silver nanoparticles as antimicrobial agents in dentistry. For instance, silver nanoparticles can be incorporated into acrylic resins for fabrication of removable dentures in prosthetic treatment, composite resin in restorative treatment, irrigating solution and obturation material in endodontic treatment, adhesive materials in orthodontic treatment, membrane for guided tissue regeneration in periodontal treatment, and titanium coating in dental implant treatment. Although not all authorities have acknowledged the safety of silver nanoparticles, no systemic toxicity of ingested silver nanoparticles has been reported. A broad concern is their potential hazard if they are released into the environment. However, the interaction of nanoparticles with toxic materials and organic compounds can either increase or reduce their toxicity. This paper provides an overview of the antibacterial use of silver nanoparticles in dentistry, highlighting their antibacterial mechanism, potential applications and safety in clinical treatment.

Keywords: antibacterial, dentistry, nanoparticles, nanotechnology, silver


Nanotechnology is defined as the design, characterization and application of structures, devices and systems by controlling shape and size at a nanometer scale (1 nm to 100 nm).1 It is an emerging field of research, with various applications in science and technology, particularly for developing new materials. Nanoparticles are developed with unique properties that make them desirable in materials science and biology.1 Among various nanoparticles, silver nanoparticles have been one of the most popular objects of study in recent decades.2 Silver nanoparticles contain 20 to 15,000 silver atoms, and their diameters are usually smaller than 100 nm. Due to a large surface-to-volume ratio, silver nanoparticles exhibit remarkable antimicrobial activity, even at a low concentration.3 In addition, they are low cost and have shown low cytotoxicity and immunological response.4 Therefore, silver nanoparticles have multiple potential biomedical applications. They are used for drug delivery, medical imaging and molecular diagnostics.5 They are also used in therapeutics, such as surgical mesh, fabrication of artificial joint replacements, wound dressing and medicament for promotion of wound healing.6

In dentistry, silver nanoparticles are used to develop antibacterial materials to improve the quality of the dental appliance for a better treatment outcome. They can be incorporated to acrylic resins for fabrication of removable dentures in prosthetic treatment, composite resin for direct restoration in restorative treatment, irrigating solution and obturation material in endodontic treatment, adhesive materials in orthodontic treatment, membrane for guided tissue regeneration in periodontal treatment, and titanium coating in dental implant treatment.7 This study provides an overview of the antibacterial use of silver nanoparticles in dentistry, highlighting their antimicrobial mechanism, applications and safety in clinical treatment.

Antibacterial Mechanism of Silver Nanoparticles

Although the exact mechanism of silver nanoparticles’ antibacterial effects has not been entirely clarified, various antibacterial actions have been proposed in Figure 1. Silver nanoparticles can continually release silver ions, which may be considered the mechanism of killing microbes.7 Owing to electrostatic attraction and affinity to sulfur proteins, silver ions can adhere to the cell wall and cytoplasmic membrane. The adhered ions can enhance the permeability of the cytoplasmic membrane and lead to disruption of the bacterial envelope.8 After the uptake of free silver ions into cells, respiratory enzymes can be deactivated, generating reactive oxygen species but interrupting adenosine triphosphate production.9 Reactive oxygen species can be a principal agent in the provocation of cell membrane disruption and deoxyribonucleic acid (DNA) modification. As sulfur and phosphorus are important components of DNA, the interaction of silver ions with the sulfur and phosphorus of DNA can cause problems in DNA replication, cell reproduction, or even result in termination of the microorganisms. Moreover, silver ions can inhibit the synthesis of proteins by denaturing ribosomes in the cytoplasm.10

Figure 1 The antibacterial actions of silver nanoparticles (AgNPs). 1) Disruption of cell wall and cytoplasmic membrane: silver ions (Ag+) released by silver nanoparticles adhere to or pass through cell wall and cytoplasmic membrane. 2) Denaturation of ribosomes: silver ions denature ribosomes and inhibit protein synthesis. 3) Interruption of adenosine triphosphate (ATP) production: ATP production is terminated because silver ions deactivate respiratory enzyme on cytoplasmic membrane. 4) Membrane disruption by reactive oxygen species: reactive oxygen species produced by the broken electron transport chain can cause membrane disruption. 5) Interference of deoxyribonucleic acid (DNA) replication: silver and reactive oxygen species bind to deoxyribonucleic acid and prevent its replication and cell multiplication. 6) Denaturation of membrane: silver nanoparticles accumulate in the pits of cell wall and cause membrane denaturation. 7) Perforation of membrane: silver nanoparticles directly move across cytoplasmic membrane, which can release organelles from cell.

In addition to being able to release silver ions, silver nanoparticles can themselves kill bacteria. Silver nanoparticles can accumulate in the pits that form on the cell wall after they anchor to the cell surface.11 The accumulated silver nanoparticles can cause cell membrane denaturation. Silver nanoparticles also have the ability to penetrate bacterial cell walls and subsequently change the structure of the cell membrane, because of their nanoscale size.11 The denaturation of cytoplasmic membrane can rupture organelles, and even result in cell lysis. In addition, silver nanoparticles can be involved in bacterial signal transduction. Bacterial signal transduction is affected by phosphorylation of protein substrates, and nanoparticles can dephosphorylate tyrosine residues on the peptide substrates. Disruption of the signal transduction can lead to cell apoptosis and termination of cell multiplication.12

The dissolution status of silver nanoparticles in exposure media strongly affects their antibacterial effect and mechanism. The dissolution efficiency depends on synthetic and processing factors, such as intrinsic silver nanoparticle characteristics and surrounding media.13 The influence of particle size and shape on the release of silver ions is described, in theoretical terms, by the Ostwald–Freundlich equation. Smaller silver nanoparticles with spherical or quasi-spherical format are more prone to silver release, due to their larger surface area.14 This also explains the lower silver release of aggregated nanoparticles, relative to isolated nanoparticles.13 Capping agents are used to modify the surfaces of silver nanoparticles, which can change their dissolution behavior.15 Apart from these intrinsic properties of silver nanoparticles, the surrounding media can influence the release of silver ions. The presence of organic or inorganic components in media can affect the dissolution of silver nanoparticles by aggregating with silver nanoparticles or complexing with silver ions. Researchers have also demonstrated that silver nanoparticles release silver ions faster in acidic solution than they do in neutral solution.16

Gram-negative bacteria are more susceptible to silver nanoparticles.17 The cellular wall of gram-negative bacteria is narrower than that of gram-positive strains. The thick cellular wall may reduce the penetration of nanoparticles into cells.17 The different antibacterial effects of silver nanoparticles on gram-negative and gram-positive bacteria suggest that uptake of silver nanoparticles is important to the antibacterial effect.13 It is commonly acknowledged that silver nanoparticles smaller than 10 nm can directly alter cell permeability, enter bacterial cells and cause cell damage.

Biofilm rapidly forms in the oral environment and protects bacteria from both silver ions and nanoparticles by hindering their transport. Researchers have found that 100% viability loss of bacteria did not occur in biofilm when silver nanoparticles with the same concentration kill all planktonic bacteria.18 Therefore, the biofilm is tolerant of the silver nanoparticles, due to its complicated architecture. Silver nanoparticles’ diffusion coefficients, which are generally related to size and physicochemical characteristics, determine their mobility and bioavailability in biofilm. First, these coefficients decrease with increasing molar mass, which means that it is harder for larger silver nanoparticles to penetrate biofilm.19 Transport through biofilm can be greatly obstructed for particles larger than 50 nm. Second, the nanoparticles’ chemical composition can arouse adsorption and accumulation of silver nanoparticles in the biofilm, thereby reducing their diffusion. Third, electrostatic interaction between bacteria and silver nanoparticles can influence charged nanoparticles’ penetration through biofilm.20

Clinical Applications of Silver Nanoparticles in Dentistry

Research on silver nanoparticles has become an emerging field in recent years. The first reason for this is that silver nanoparticles can be synthesized by mediating their nucleation and growth process, using different synthetic reagents. Second, silver nanoparticles can be specifically functionalized with molecular capping agents, such as proteins and chemical groups. Third, silver nanoparticles possess a strong antibacterial effect that improves clinical treatment outcomes.13 Therefore, homogenous silver nanoparticles with controlled size, morphology and function can be regarded as multifunctional building blocks in diverse dental materials. Silver nanoparticles can be added to acrylic resins for fabrication of removable dentures in prosthetic treatment, composite resin for direct restoration, irrigating solution and obturation material in endodontic treatment, adhesive materials in orthodontic treatment, membrane for guided tissue regeneration in periodontal treatment, and titanium coating in dental implant treatment (Figure 2).

Figure 2 The antibacterial application of silver nanoparticles in dentistry.

Prosthetic Treatment

Acrylic resin is commonly used to fabricate removable dentures. Opportunistic oral pathogens can colonize acrylic materials, causing dental infections, such as denture stomatitis. Silver nanoparticles can be added to acrylic resin to inhibit the growth of such bacteria as Streptococcus mutans, Escherichia coli and Staphylococcus aureus.21 In addition to the antibacterial effects, acrylic resin incorporated with silver nanoparticles has also displayed antifungal properties against the adhesion of Candida albicans, which is one of the key opportunistic pathogens on a denture base.22

Silver nanoparticles can also enhance the mechanical properties of acrylic resin. The effect of silver nanoparticles on the mechanical properties is influenced by the concentration of silver nanoparticles, the type of acrylic resin and the polar interactions between the polymethyl methacrylate chains and silver nanoparticles.23 Silver nanoparticles can increase the flexural strength and elastic modulus of acryl resin. Moreover, they can increase the thermal conductivity and compressive strength of the acrylic base.24

Restoration Treatment

Residual bacteria in the prepared tooth cavity and colonized bacteria in microleakages along tooth–restoration interfaces may result in secondary caries of the dental restoration. Silver nanoparticles can be added to adhesive systems and composite resins. They can prevent secondary caries by bringing notable antimicrobial properties to bear at low concentration.25 Restorative adhesives containing silver nanoparticles can interfere with biofilm formation, due to the widespread inhibitory action of cariogenic bacteria. Composite resin with silver nanoparticles had no significant adverse effect on fibroblasts, because the silver ions released per day are very low.26 In addition, researchers immobilized silver nanoparticles in a biocompatible film of polymers to obtain a maximum lethal effect on microbial cells without killing human cells.

Silver nanoparticles can also be added to porcelain to enhance its mechanical properties. Porcelain’s reinforced mechanical properties, such as hardness and fracture toughness, can be attributed to the residual compressive stress generated by an ion-exchange reaction and differential thermal expansion of silver nanoparticles.27

Endodontic Treatment

Silver nanoparticles can be used as a substitute of sodium hypochlorite in intracanal irrigation for endodontic treatment. Gutta-percha coated with silver nanoparticles has been developed as an antimicrobial obturator for root canal obturation. Silver nanoparticles are also incorporated as antibacterial material into mineral trioxide aggregate to enhance the success of pulp-capping, apexification and sealing perforations in teeth.

Sodium hypochlorite is regarded as the gold standard for chemical disinfection of root canals in endodontic treatment.28 However, sodium hypochlorite reduces the elastic modulus and flexural strength of dentine and causes toxic damage to the periapical tissues. A study found that root canal irrigation with silver nanoparticle solution did not significantly affect the mechanical properties of dentine.29 Another study found that a low concentration of silver nanoparticles had superior biocompatibility, relative to sodium hypochlorite. In addition, silver nanoparticle-based irrigation solution was as potent as sodium hypochlorite in the elimination of both Enterococcus faecalis and Staphylococcus aureus. Therefore, some have suggested using silver nanoparticle solution for root canal irrigation during endodontic treatment.

Gutta-percha is commonly used in root canal obturation. Although it is desirable that endodontic obturators have antibacterial properties, gutta-percha’s antibacterial properties are limited. A study found gutta-percha coated with silver nanoparticles had antibacterial and antifungal properties and was as effective as conventional gutta-percha in preventing bacterial leakage.30 In addition, there is no difference in the cytotoxicity of fibroblasts and subcutaneous tissue reaction or inflammation between gutta-percha-coated silver nanoparticles and conventional gutta-percha.31

Mineral trioxide aggregate has extensive applications in dentistry, such as pulp-capping, apexification and sealing perforations. It is essential to add antimicrobial agents into mineral trioxide aggregate to enhance its antimicrobial properties. Mineral trioxide aggregate, combined with silver nanoparticles, can enhance antibacterial activity against anaerobic endodontic pathogens. The antifungal ability against Candida albicans was also improved by the addition of silver nanoparticles to mineral trioxide aggregate. Moreover, research has reported that mineral trioxide aggregate, incorporated with silver nanoparticles, can increase pH value and compressive strength with proper radiopacity and shorter setting time.32 In addition, researchers have revealed that mineral trioxide aggregate with silver nanoparticles has good biocompatibility without inducing inflammatory reaction.33

Orthodontic Treatment

Silver nanoparticles can be used to prevent enamel caries (white spot lesion), which is a common complication for patients with orthodontic treatment. Silver nanoparticles can be added to adhesive materials, such as resin-modified glass ionomers and composite adhesives, to inhibit biofilm growth. Orthodontic elastomeric modules, such as ligatures, can be allied with silver nanoparticles to prevent enamel caries.34 They can affect the adhesion of Streptococcus mutans to the materials by contact with bacteria and inhibit bacterial action around brackets and wires by the release of silver ions.35 In addition, the materials can release silver ions for 4 months to exert a long-term antibacterial effect. Materials with silver nanoparticles are biocompatible and have no significant cytotoxic or mutagenic effects. Moreover, the materials have negligible irritation and delayed-type hypersensitivity potential. There are no adverse effects on their mechanical properties, such as shear bond strength.36

Dental Implant Treatment

Peri-implant infection is a major threat for implant treatment. Conventional antibacterial methods are insufficient, due to constant risk of infection from peri-operation, which persists until later in life.37 Modifying implant surfaces with silver nanoparticles, using various doped methods, is currently an area of intensive interest, due to the safety and strong antibacterial properties of silver nanoparticles.38

Researchers have demonstrated the long-term antibacterial effect of titanium implants coated with silver nanoparticles by steadily controlling silver ion release with various coating methods.39 The antibacterial effect can also be prolonged by immobilizing silver nanoparticles on implant surfaces. The embedded silver nanoparticles inhibit bacteria without consumption through continuous direct contact with bacterial cells. Although decreasing the size of silver nanoparticles generally increases their antimicrobial ability, larger-sized silver nanoparticles embedded in titanium had a better antibacterial effect, due to the consumption of more protons.40 Silver nanoparticles can kill Staphylococcus aureus and Pseudomonas aeruginosa at a low concentration, which has no significant cytotoxic effects on osteoblastic cells.41 In addition, titanium embedded with silver nanoparticles can enhance bone mineral density, bone formation and trabecular pattern, with no harm to tissues adjacent to dental implants.40

Periodontal Treatment

Periodontitis is a prevalent chronic inflammatory disease caused by various types of microorganisms. Adequate infection control is essential for periodontal treatment through disruption of the biofilm and suppression of inflammation.42 Compared to traditional antibiotics, silver nanoparticles possess antibacterial properties without the generation of bacterial resistance.43 Silver nanoparticles significantly enhance bactericidal properties when combined with antibiotics, including cefotaxime, ceftazidime, meropenem and ciprofloxacin. Importantly, inactive antibiotics restore strong antibacterial activity against multi-resistant bacterial strains when they are combined with silver nanoparticles.43 Silver nanoparticles synthesized with an appropriated capping agent can promote the inhibition effect against gram-negative bacteria that predominantly cause periodontal infections. Smaller-sized silver nanoparticles presented a higher antibacterial property against oral anaerobic pathogenic bacteria. Because guided tissue regeneration membrane with silver nanoparticles reduced adherence and penetration of bacteria, treatment of intra-bony defects using guided tissue regeneration membrane with silver nanoparticles can improve clinical success.44

Silver nanoparticles were reported to possess an anti-inflammatory effect, through modulation of the level of inflammatory cytokines and growth factors.45 Periodontal dressing coated with silver nanoparticles can be used to treat gingival wounds. This treatment accelerates the early phases of wound healing, with apparently new collagen synthesis and neovascularization. However, some silver nanoparticles can provoke inflammation and foreign body reaction in periodontal dressing.46

Safety of Silver Nanoparticles in Dental Applications

Some researchers are concerned about the toxicity of silver nanoparticles. The toxicity of silver nanoparticles is directly associated with the free silver ions.13 Because of the nanoscale size of silver nanoparticles, they can readily disturb biological molecules, cells and human organs. Some laboratory studies have reported that silver nanoparticles can induce oxidative stress and impair mitochondrial function of human cells.47 Moreover, silver can be detected in organs after administration of massive silver nanoparticle doses, especially in the liver and spleen. Researchers are also concerned about the ability of silver nanoparticles to cross the blood-brain barrier, via trans-synaptic transport, and accumulate in the brain.48

However, even at low concentration, small-size silver nanoparticles decrease some inflammatory cytokines and angiogenesis parameters. Silver nanoparticles can express anti-inflammatory properties. They are biocompatible with fibroblasts and keratinocyte. Silver accumulated in organs can mostly be cleared after 8 weeks.19 An animal study using rats found no observable adverse effect with the oral administration of silver nanoparticles. Another clinical study did not find clinically important observable toxic effects on patients taking commercial colloidal silver products.11 Researchers discovered mineral trioxide aggregate with silver nanoparticles did not induce a significant inflammatory reaction on subcutaneous tissues of rats. It has also been proven that periodontal dressing with a high concentration of silver nanoparticles is biocompatible with gingival wound healing.49 Although authorities have not fully acknowledged the safety of silver nanoparticles, clinicians often apply silver nanoparticle-based wound dressings to their patients’ wounded skin.50 Silver nanoparticles can enter into the body through a wound because the strict barrier is lost. These silver nanoparticles are phagocytosed by macrophages and may cause immune system perturbations. Silver nanoparticles also enter other organs, such as the liver and spleen, via circulation. Nevertheless, the systemic toxicity of ingested silver nanoparticles has not been reported. Another concern is their potential hazard to the marine life if they are released into the environment. However, the toxicity of silver nanoparticles may be increased or reduced when they interact with materials and organic compounds. Hence, nanoparticles can have a harmful or helpful effect on the environment. It is essential to perform a nanomaterial-specific evaluation to ensure their use is safe for humans and the environment.


Incorporating silver nanoparticles into dental materials may enhance the mechanical features and antibacterial properties of the dental materials. Although the mechanism of silver nanoparticles’ antibacterial effects is not yet fully understood, many researchers believe that silver nanoparticles can continually release silver ions to kill microbes. An increasing number of dental materials with silver nanoparticles are being developed for prosthetic, restorative, endodontic, orthodontic, periodontal and implant treatment. Some laboratory studies reported that silver nanoparticles have cytotoxic effects on human cells. However, the clinical significance of the potential toxicity of silver nanoparticles remains unknown. Further studies are essential because clinical evidence is still limited.


This study is supported by the Hong Kong University Grant Council General Research Fund (No. 17100218) and the National Natural Science Foundation of China (NSFC) – General Program (2018) No. 8187082.


The authors declare that they have no conflicts of interest in this work.


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