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Targeting polyelectrolyte networks in purulent body fluids to modulate bactericidal properties of some antibiotics

Authors Bucki R, Durnaś B, Wątek M, Piktel E, Cruz K, Wolak P, Savage PB, Janmey PA

Received 1 July 2017

Accepted for publication 30 October 2017

Published 11 January 2018 Volume 2018:11 Pages 77—86


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Professor Suresh Antony

Robert Bucki,1,* Bonita Durnaś,2,* Marzena Wątek,2,3 Ewelina Piktel,1 Katrina Cruz,4 Przemysław Wolak,2 Paul B Savage,5 Paul A Janmey4

1Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Białystok, 2Department of Microbiology and Immunology, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, 3Holy Cross Oncology Center of Kielce, Kielce, Kielce, Poland; 4Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, 5Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA

*These authors contributed equally to this work

Abstract: The response of the human immune system to most bacterial infections results in accumulation of neutrophils at infection sites that release a significant quantity of DNA and F-actin. Both are negatively charged polyelectrolytes that can interact with positively charged host defense molecules such as cathelicidin-delivered LL-37 peptide or other cationic antibiotic agents. Evaluation of the ability of bacterial outgrowth (using luminescence measurements or counting colony-forming units) to form a biofilm (quantified by crystal violet staining) and analysis of the structure of DNA/F-actin network by optical microscopy in human pus samples treated with different antibiotics in combination with plasma gelsolin, DNAse 1, and/or poly-aspartic acid revealed that bactericidal activity of most tested antibacterial agents increases in the presence of DNA/F-actin depolymerizing factors.

Keywords: antibiotic activity, polyelectrolyte network, depolymerizing factors, cathelicidin, ceragenins, DNase 1, cystic fibrosis

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