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STAT3 induces colorectal carcinoma progression through a novel miR-572-MOAP-1 pathway

Authors Wang N, He X, Zhou R, Jia G, Qiao Q

Received 3 December 2017

Accepted for publication 20 April 2018

Published 15 June 2018 Volume 2018:11 Pages 3475—3484

DOI https://doi.org/10.2147/OTT.S158764

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 4

Editor who approved publication: Prof. Dr. Geoffrey Pietersz


Nan Wang,1 Xianli He,1 Ru Zhou,2 Guozhan Jia,1 Qing Qiao1

1Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shanxi, 710038, China; 2Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi’an, Shanxi, 710032, China

Purpose: Colorectal carcinoma (CRC) is among the most common causes of death. Recent studies have shown that both STAT3 and miR-572 contribute to CRC progression. STAT3 plays an important role in miRNA expression. Moreover, MOAP-1, which is a pro-apoptotic protein that induces cell death or apoptosis, has a direct correlation with miRNA. Therefore, the current study is designed to explore whether miR-572 and STAT3 are involved in a common pathway and the role of MOAP-1 in this process.
Patients and methods: The expressions of STAT3, miR-572, and MOAP-1 in human CRC tissues and multiple cell lines were estimated by qRT-PCR or Western blot. MTT, transwell migration, and invasion assays were used to assess cell growth, migration, and invasion, respectively. Dual-luciferase reporter assay was applied to examine the association between miR-572 and MOAP-1.
Results: Elevated STAT3 levels were accompanied by increased miR-572 and decreased MOAP-1 levels in primary CRC specimens and cell lines. STAT3 promoted CRC cell growth, migration, and invasion via the upregulated expression of miR-572. Subsequently, miR-572 inhibited MOAP-1 protein expression through an interaction with its 3'UTR.
Conclusion: Our study proposes a novel STAT3-miR-572-MOAP-1 pathway involved in the process of CRC progression, which might be a potential target for the development of new preventive and therapeutic approaches against human colorectal cancer.

Keywords:
colorectal neoplasm, STAT3, MOAP-1, miR-572, tumor progression

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