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Spoligotype and Drug Susceptibility Profiles of Mycobacterium tuberculosis Complex Isolates in Golestan Province, North Iran

Authors Mansoori N, Vaziri F, Amini S, Khanipour S, Pourazar Dizaji S, Douraghi M

Received 28 March 2020

Accepted for publication 18 June 2020

Published 1 July 2020 Volume 2020:13 Pages 2073—2081

DOI https://doi.org/10.2147/IDR.S255889

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Professor Suresh Antony


Noormohamad Mansoori,1 Farzam Vaziri,2,3 Sirus Amini,4 Sharareh Khanipour,2,3 Shahin Pourazar Dizaji,2,3 Masoumeh Douraghi1,5

1Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; 2Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; 3Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran; 4Regional Tuberculosis Reference Laboratory, Tehran University of Medical Sciences, Tehran, Iran; 5Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran

Correspondence: Masoumeh Douraghi
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, PO Box: 14155-6446, Tehran, Iran
Tel +98 21 42933152
Fax +98 21 88954913
Email mdouraghi@tums.ac.ir

Introduction: Despite the moderate incidence of tuberculosis (TB) in many parts of Iran, Golestan province had a permanently higher TB incidence rate than the national average. Moreover, Golestan province receives immigrants, mainly from TB-endemic areas of Iran and neighbor countries. Here, we aimed to characterize the circulating Mycobacterium tuberculosis complex (MTBC) isolates in terms of the spoligotype and drug resistance patterns, across Golestan province.
Materials and Methods: A set of 166 MTBC isolates was collected during July 2014 to July 2015 and subjected to drug susceptibility testing for first- and second-line anti-TB drugs and spoligotyping.
Results: Of 166 MTBC isolates, 139 (83.7%) isolates were assigned to 28 spoligotype international types (SITs). The most frequent SITs were SIT127/Ural-2 (n=25, 15.1%), followed by SIT1/Beijing (n=21, 12.7%) and SIT3427/Ural-2 (n=18, 10.8%). The set of 18 isolates (10.8%) showed resistance to at least one drug, which mainly belonged to SIT1/Beijing (n=7, 38.9%), orphan patterns (n=4, 22.2%) and SIT357/CAS1-Delhi (n=3, 16.7%). In addition, four isolates (2.4%) were resistant to pyrazinamide. The analysis of mutation corresponded to resistance to rifampin and isoniazid showed that two isolates had Ser531Leu substitution in rpoB, four isolates had Ser315Thr substitution in katG and one isolate had [C(− 15)T] in inhA locus.
Conclusion: High diversity in spoligotypes of the MTBC isolates and lack of dominant genotype might be due to residence of immigrants in this region and consequent reactivation of latent infection. In addition, due to the presence of extensively drug-resistant (XDR) isolates in Golestan province, it is important to conduct future studies to determine transmission pattern of drug-resistant isolates in this region.

Keywords: Mycobacterium tuberculosis, tuberculosis, spoligotyping, genotyping, transmission

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