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Sodium bicarbonate ingestion improves Yo-Yo intermittent recovery test 1 performance: a randomized crossover trial

Authors Dixon H, Baker CE, Baker JS, Dewhurst S, Hayes LD

Received 8 January 2017

Accepted for publication 21 March 2017

Published 28 April 2017 Volume 2017:9 Pages 23—27

DOI https://doi.org/10.2147/NDS.S131947

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 3

Editor who approved publication: Dr Gary Johanning


Helen Dixon,1 Catherine E Baker,2 Julien S Baker,3 Susan Dewhurst,4 Lawrence D Hayes4

1School of Human Sciences, London Metropolitan University, London, 2English Institute of Sport, Bisham Abbey National Sports Center, Buckinghamshire, 3Institute of Clinical Exercise and Health Science, University of the West of Scotland, Hamilton, 4Department of Medical and Sport Sciences, University of Cumbria, Lancaster, UK

Abstract: This study investigated the effect of sodium bicarbonate (NaHCO3) ingestion on the Yo-Yo intermittent recovery test 1 (IR1). We tested the hypothesis that acute ingestion of NaHCO3 would increase blood lactate concentrations [BLa], enhance performance, and reduce rating of perceived exertion (RPE) in the Yo-Yo IR1. Eight recreationally active males (N=8, age: 26±4 yr, height: 178±6 cm, body mass: 82±10 kg) participated in the Yo-Yo IR1 on two separate occasions, separated by 1 wk, in a randomized crossover design. Following familiarization, during seated rest, participants’ pretest [BLa] was taken, and participants then consumed either a placebo of 0.3 g·kg–1 body weight sodium chloride or 0.3 g·kg–1 body weight NaHCO3. Sixty minutes postingestion, a standardized warm-up preceded the Yo-Yo IR1. Upon completion, postexercise [BLa] (mmol·L–1), RPE (arbitrary units) and Yo-Yo IR1 time to fatigue (s) were recorded. Paired t-test revealed a small but significant improvement in Yo-Yo IR1 performance under the NaHCO3 condition (610±267 sec), compared to the placebo condition (556±259 sec; p=0.01; Cohen’s d=0.20). [BLa] increased more under the NaHCO3 condition (1.6±0.7 to 17.5±5.2 mmol·L–1; p<0.001; Cohen’s d=4.29), compared to the placebo condition (2.0±0.7 to 11.5±5.0 mmol·L–1; p=0.001; Cohen’s d=2.66). Postexercise RPE was not significantly different between conditions. The results of this study suggest that acute NaHCO3 ingestion improves Yo-Yo IR1 performance without altering RPE, likely through an increased lactate efflux, demonstrated by increased [BLa].

Keywords: alkalosis, anaerobic, blood lactate, ergogenic aid, performance, repeated sprints

Introduction

Muscular performance declines during prolonged and intense activity, with changes in metabolites contributing to reductions in force production, muscle shortening velocity, and prolongation of relaxation.1 Mechanisms such as acid excretion via the kidneys, hyperventilation, and blood bicarbonate (HCO3) operate to offset increased production and release of hydrogen (H+) during times of accelerated metabolism.2 Changes in muscle pH have been suggested to play a critical role in fatigue,3 while reduced ability of muscle to maintain force may be related to accumulating H+.4 However, in a recent special communication, Westerblad5 suggested that while temporal correlations between change in intracellular pH and decrease in muscle force have been observed, recovery of force in the absence of pH recovery has been observed.

Intermittent high-intensity exercise decreases blood and muscle pH, ostensibly contributing to skeletal muscle fatigue.3 Repeated high-intensity exercise performance therefore depends largely upon metabolic buffering capacity.6 Sodium bicarbonate (NaHCO3) is an ergogenic aid, employed by athletes prior to exercise, to increase buffering capacity of H+ ions, in an attempt to delay fatigue.7 While muscle cell membranes are impervious to HCO3, increasing extracellular HCO3 increases the pH gradient between intracellular and extracellular environments.8 As a result, significantly higher blood lactate concentrations [BLa] have been observed following NaHCO3 supplementation,9 suggesting NaHCO3 increases the efflux of H+ and La from working muscle into general circulation. As such, during specific exercise protocols (those involving a large anaerobic component), NaHCO3 may be efficacious in enhancing exercise performance.10

Lavender and Bird11 reported that peak power output during 10-sec sprints on a cycle ergometer was significantly greater following NaHCO3 ingestion compared to a placebo. Yet Vanhatalo et al12 observed that despite pre-exercise alkalosis being achieved by NaHCO3 ingestion, critical power, work done above the critical power (W′: considered as energy available from anaerobic sources), and total work done during a 3-min all-out test were unaltered. However, performance enhancement has been demonstrated during repeated sprint swimming,13 and high-intensity running14 was preceded by NaHCO3 ingestion. Moreover, some authors have reported improved performance during the Yo-Yo intermittent recovery test 2 (IR2).15,16 However, some ambiguity remains regarding the effectiveness of NaHCO3 ingestion prior to the Yo-Yo test.17

Given that numerous sporting events are characterized by repeated periods of intense anaerobic activity,18,19 tolerance to repeated bouts of intense exercise is necessary. As proposed by Bangsbo et al,18 the Yo-Yo IR1 appears well suited to this purpose. The test is conducted to volitional exhaustion, or an inability to maintain running speed. Repeated sprints of progressively increasing velocities are required, interspersed with 10 sec of active recovery. This test has been proposed as an estimation of match-related fitness in handball,20 soccer,21 and rugby league.22

While the influence of NaHCO3 during the Yo-Yo IR2 is well characterized,15,16 there remains a paucity of data concerning the effect of NaHCO3 ingestion prior to the Yo-Yo IR1. Therefore, the aim of the present investigation was to examine the influence of acute NaHCO3 ingestion on [BLa], rating of perceived exertion (RPE), and performance during the Yo-Yo IR1.

Methods

Participants

In a randomized, single-blinded, repeated measures crossover design, participants reported to the laboratory following familiarization on two separate occasions, separated by 1 wk with a 7-day washout period considered sufficient to ameliorate the residual effects of NaHCO3.14 Testing was completed at the same time of day (19:00–20:00 h) to abrogate the influence of diurnal variation in exercise performance.23

Prior to enrollment, participants completed a physical activity readiness questionnaire and provided written informed consent. The investigation was approved by the London Metropolitan University Research Ethics Committee. An a priori power calculation using mean ± standard deviation (SD) from previous literature15 suggested a sample size of N=6 would be adequate to obtain statistical power of 0.8 for a two-tailed test. Therefore, to allow for dropouts, eight recreationally active males (age: 26±4 yr, body height: 178±6 cm, body mass: 82±10 kg) volunteered to participate in the study. Participants had abstained from exercise and alcohol for the previous 24 h.

Experimental procedures

Participants arrived at the laboratory 3–4 h postprandial; thereafter body height (Seca Leicester Height Measurer; Birmingham, UK) and mass (Salter Touch Analyser Digital Scales; Kent, UK) were recorded. On each occasion, [BLa] concentration was measured at rest and postexercise, using a handheld portable analyzer measuring whole blood (LactatePro 2; Arkray, Kyoto, Japan). Following piercing of the skin using a retractable lancet, a 0.3-µL blood sample was obtained from the fingertip and drawn directly from the sample site into the coded reagent strips of the device. This method has demonstrated interclass correlations (ICCs) of 0.989.24 Following resting samples, participants consumed a 500 mL, sugar-free beverage containing either 1) 0.3 g·kg–1 body weight NaHCO3 or 2) an equimolar amount of the placebo, sodium chloride. This dosage strategy has previously been used by Higgins et al.25 Sodium chloride was consumed to ensure participants’ inability to identify the NaHCO3 solution, as previously demonstrated.14

Yo-Yo IR1

Sixty minutes postingestion, participants completed a standardized 5-min warm-up involving walking at a self-selected pace, interspersed with two 5- to 10-sec all-out sprints. A recovery period of 2 min was permitted between warm-up and test. The Yo-Yo IR1 requires participants to perform 2 × 20 m shuttle runs at speeds indicated by beeps on an audio recording, interspersed by 10-sec active recovery, 5 m behind the starting marker. Participants performed the Yo-Yo IR1 until volitional exhaustion, or when participants were incapable of maintaining the designated running speed. On the first occasion, participants were unable to maintain the designated running speed, a warning was given from the researcher, and on the second time, participants were withdrawn from the test. When individuals twice failed to reach the finishing line in the time allocated, or reached volitional exhaustion, cumulative time (sec) was recorded. Following test completion, RPE26 were recorded within 30 sec, and [BLa] concentrations were collected within 2 min. The Yo-Yo IR1 has demonstrated reliability and validity in measurement of match-related fitness,20 capable of distinguishing between competition level, playing position, and training adaptation.27 A recent investigation observed ICCs of 0.82–0.92.28

Statistical analysis

Data were analyzed using SPSS (version 22; IBM North America, New York, NY, USA). To determine parametricity, Levene’s tests (homogeneity of variance) and Shapiro–Wilk (normal distribution) were employed. Where parametric assumptions were met, data were analyzed using a paired samples t-test to examine differences in Yo-Yo IR1 performance between conditions. A 2 × 2 (time point × condition) repeated measures analysis of variance (ANOVA) was used to test for differences in [BLa], and a Wilcoxon signed rank test was used to examine differences between conditions for RPE. In all instances, significance was set a priori at p<0.05, and effect size is reported for primary outcome measures. Effect sizes are reported as eta squared (η2) in instances with more than one comparison, and Cohen’s d for paired comparisons. Effect sizes are considered trivial (<0.2), small (0.2–0.49), medium (0.5–0.79), or large (≥0.8).29 Data are presented as mean ± SD in all instances.

Results

Due to 100% adherence, eight participants (N=8) completed both conditions, and statistical power was confirmed as 0.981 for cumulative time. Paired samples t-test revealed significantly improved Yo-Yo IR1 under the NaHCO3condition compared to placebo (610±267 sec and 556±259 sec, respectively: p=0.01; Cohen’s d=0.20 [Figure 1A]). Postexercise RPE was not significantly different (p=1.00) between NaHCO3 and placebo conditions (16±2 and 16±1 for NaHCO3 and placebo, respectively [Figure 1B]).

Figure 1 Performance and RPE during the Yo-Yo IR1 following NaHCO3 ingestion.


Notes: (A) Cumulative time completed and (B) rating of perceived exertion, during the Yo-Yo IR1 in a group of recreationally active males (n=8) following sodium bicarbonate (NaHCO3) or placebo ingestion. Data are presented as individual lines and mean ± SD. ¥ denotes significantly increased compared to placebo (p=0.01).


Abbreviations: IR1, intermittent recovery test 1; SD, standard deviation.

NaHCO3 and placebo pretest [BLa] were not significantly different (p=0.209). A significant, large effect of time point (p=0.002; η2=0.758) and condition (p<0.001; η2=0.900) was observed for [BLa] (Figure 2). Moreover, a significant, large interaction effect (p=0.001; η2=0.824) was detected. Blood lactate increased more under NaHCO3 (1.6±0.7 to 17.5±5.2 mmol·L–1; p<0.001; Cohen’s d=4.29) compared to placebo (2.0±0.7 to 11.5±5.0 mmol·L–1; p=0.001; Cohen’s d=2.66).

Figure 2 Blood lactate concentrations samples pre- and post-Yo-Yo intermittent recovery test 1 in a group of recreationally active males (n=8) following sodium bicarbonate (NaHCO3) or placebo ingestion.


Notes: Data are presented as individual lines and mean ± SD. Dashed lines represent individual response to placebo, whereas solid lines represent individual response to NaHCO3. ¥ denotes significantly increased compared to placebo (p<0.01).


Abbreviation: SD, standard deviation.

Discussion

The present study examined the effects of acute NaHCO3 ingestion on Yo-Yo IR1 performance, [BLa], and RPE. The primary finding of this investigation was that acute supplementation of NaHCO3 improved Yo-Yo IR1 performance. Moreover, greater [BLa] was observed postexercise under the NaHCO3 condition compared to placebo.

The present study is in line with previous investigations in reporting a 52% increase in [BLa] following NaHCO3 ingestion compared to placebo. Bishop and Claudius14 reported [BLa] increased 26% following NaHCO3 ingestion. Differences in the magnitude of [BLa] increase may be explicated by dissimilar exercise protocols, as the present investigation examined the influence of NaHCO3 ingestion on Yo-Yo IR1 performance, whereas the study by Bishop and Claudius14 investigated the effect during intermittent sprint cycling.

Substantial research exists examining the ergogenic effect of NaHCO3 on exercise performance.6,30,31 In a double-blinded, crossover study, Hobson et al31 examined the performance of 20 male rowers undertaking two 2,000 m rowing time trials, and reported 0.3 g·kg–1 NaHCO3 enhanced rowing performance, particularly in the final 500 m (by 1.1±1.7 sec). Likewise, Higgins et al25 reported NaHCO3 ingestion increased cycling capacity by 17% at 100% power output determined using a 3-min stage-ramp protocol, although not at 110% or 120%, suggesting NaHCO3 ingestion significantly improves continuous constant load cycling at 100% peak minute power, but not at 110% or 120%. Goldfinch et al32 similarly reported six male athletes ran 400 m significantly faster following 0.4 g·kg–1 body weight NaHCO3 compared to placebo (56.94±2.25 sec vs 58.63±2.25 sec for NaHCO3 and placebo, respectively).

Recently, NaHCO3 supplementation has garnered interest in relation to intermittent sprint performance, as this modality is more representative of sprint profiles of team sports.33 Glaister33 suggested the ability to repeat maximal, short-duration sprint efforts, while resisting fatigue is a determining factor in many team sports. Although mean heart rate and oxygen uptake (VO2) during team sports are approximately 80% and 70% maximum, respectively,3436 expressing intensity as an average during these events belies the complexity of physiological processes. For example, in soccer, high-intensity movements typically last ~4 sec, and ~22 m, although this is position dependent.37 Despite the intricacies of determining intensity of repeated sprints during match play, it is evident that repeated sprint ability is a key determinant of field-based team sports,38 and therefore strategies to improve this ability via ergogenic aids are of interest to practitioners and athletes alike.

The present investigation reports a 9.7% improvement in Yo-Yo IR1 performance in a group of recreationally active males and is therefore in line with previous research in suggesting NaHCO3 ingestion improves intermittent exercise performance.31 Krustrup et al15 reported a 14% improvement in the Yo-Yo IR2 as a result of NaHCO3 ingestion in a group of trained athletes. Data from the same laboratory suggest that when preceded by intense arm cranking exercise, NaHCO3 ingestion improved performance in the Yo-Yo IR2 by 23% compared to placebo.16

Conclusion

The present study demonstrates acute NaHCO3 ingestion improves performance in the Yo-Yo IR1 without concomitant change in perception of effort. One limitation of the present study is that we did not measure blood gas parameters, which would add confirmatory evidence that the performance enhancement observed was a result of NaHCO3 ingestion. However, given the design of our investigation (randomized crossover), and increased [BLa], we find it difficult to provide an alternative explanation. As such, the current study is the first to observe increased performance during the Yo-Yo IR1 following NaHCO3 ingestion, which adds to the body of literature supporting the ergogenic effect of NaHCO3on intermittent sprint exercise.

Disclosure

The authors report no conflicts of interest in this work.

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