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Role of Hippo/YAP signaling in irradiation-induced glioma cell apoptosis

Authors Xu X, Chen Y, Wang X, Mu X

Received 1 April 2019

Accepted for publication 6 July 2019

Published 9 August 2019 Volume 2019:11 Pages 7577—7585

DOI https://doi.org/10.2147/CMAR.S210825

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 2

Editor who approved publication: Professor Lu-Zhe Sun


Xiaofei Xu,1 Yan Chen,2 Xi Wang,1 Xingguo Mu1

1Department of Radiology, The Second Hospital of Jilin University, Chang Chun 130041, People’s Republic of China; 2Department of Neurosurgery, The Second Hospital of Jilin University, Chang Chun 130041, People’s Republic of China

Background: Although Hippo/Yes-associated protein (YAP) signaling plays crucial roles in radiation sensitivity and resistance of multiple kinds of cancers, its role in the radiation sensitivity of glioma cells remains unclear. The present study aimed to reveal Hippo/YAP role in the radiation sensitivity of glioma cells.
Methods: Glioma U251 cells were administrated with different doses of irradiation. Cell Counting Kit-8 (CCK-8) and flow cytometry assays were used to assess cell viability and apoptosis. Co-immunoprecipitation (co-IP) assay was used to assess the interactions between proteins.
Results: The results showed that irradiation exposure significantly inhibited cell viability and induced cell apoptosis in a dose-dependent manner, as well as decreased YAP1 expression via enhancing RCHY1-mediated YAP1 protein degradation. In addition, we observed that downregulation of YAP1 or RCHY1 weakened the role of irradiation exposure in cell viability inhibition and apoptosis promotion.
Conclusion: Collectively, this study emphasizes the vital role of Hippo/YAP signaling in radiation sensitivity of glioma, that RCHY1-mediated YAP1 protein downregulation is a main mechanism accounting for radiation-induced glioma cell apoptosis. Our study may enrich the theoretical basis of Hippo/YAP signaling as a new target for improving radiation sensitivity in glioma.

Keywords: radiation, Hippo/YAP signaling, ubiquitination, RCHY1, glioma

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