Reduction of COPD Hyperinflation by Endobronchial Valves Improves Intercostal Muscle Morphology on Ultrasound
Authors Wallbridge P, Hew M, Parry SM, Irving L, Steinfort D
Received 22 September 2020
Accepted for publication 16 November 2020
Published 7 December 2020 Volume 2020:15 Pages 3251—3259
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Richard Russell
Peter Wallbridge,1,2 Mark Hew,1,3,4 Selina M Parry,5 Louis Irving,1,2 Daniel Steinfort1,2
1Department of Medicine, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, Australia; 2Department of Respiratory and Sleep Medicine, Royal Melbourne Hospital, Parkville, VIC, Australia; 3Public Health and Preventive Medicine, Monash University, Clayton, VIC, Australia; 4Allergy, Asthma & Clinical Immunology, Alfred Health, Prahran, VIC, Australia; 5Department of Physiotherapy, The University of Melbourne, Parkville, VIC, Australia
Correspondence: Peter Wallbridge
Department of Respiratory Medicine, Level 5 East, Royal Melbourne Hospital, Grattan St, Parkville, VIC 3050, Australia
Tel +61 3 9342 7000
Fax +61 3 9342 3141
Background and Objectives: Parasternal intercostal ultrasound morphology reflects spirometric COPD severity. Whether this relates to the systemic nature of COPD or occurs in response to hyperinflation is unknown. We aimed to assess changes in ultrasound parasternal intercostal muscle quantity and quality (echogenicity) in response to relief of hyperinflation. We hypothesised that reduction in hyperinflation following endobronchial valve (EBV) insertion would increase ultrasound parasternal thickness and decrease echogenicity.
Methods: In this prospective cohort study, eight patients with severe COPD underwent evaluation of health-related quality of life, lung function, and sonographic thickness of 2nd parasternal intercostal muscles and diaphragm thickness, both before and after EBV insertion. Relationships between physiological and radiographic lung volumes, quality of life and ultrasound parameters were determined.
Results: Baseline FEV1 was 1.02L (SD 0.37) and residual volume (RV) was 202% predicted (SD 41%). Median SGRQ was 63.26 (range 20– 70.6). Change in RV (− 0.51 ± 0.9L) following EBV-insertion showed a strong negative correlation with change in parasternal thickness (r = − 0.883) ipsilateral to EBV insertion, as did change in target lobe volume (− 0.89 ± 0.6L) (r = − 0.771). Parasternal muscle echogenicity, diaphragm thickness and diaphragm excursion did not significantly change.
Conclusions: Dynamic changes in intercostal muscle thickness on ultrasound measurement occur in response to relief of hyperinflation. We demonstrate linear relationships between intercostal thickness and change in hyperinflation following endobronchial valve insertion. This demonstrates the deleterious effect of hyperinflation on intrinsic inspiratory muscles and provides an additional mechanism for symptomatic response to EBVs.
Keywords: endobronchial valve, COPD, ultrasound, respiratory muscle, measurement
Plain Language Summary
Parasternal intercostal thickness negatively correlates with COPD severity although contribution of hyperinflation is unclear. We demonstrate strong correlations between reduction in residual volume, reduction in CT-target lobe volume and increased parasternal thickness following endobronchial valve insertion, suggesting reduced parasternal thickness is related to hyperinflation. This provides a novel mechanism for symptomatic improvement post-EBV insertion.
Chronic obstructive pulmonary disease (COPD) is characterised by non-reversible airflow limitation,1 predisposing to lung hyperinflation with associated functional impairment. Hyperinflation has been shown to have significant impacts on dyspnoea, related to inability to appropriately increase tidal volume2–4 and increased neural respiratory muscle drive with exercise.5 Reduction in the degree of hyperinflation with heliox6 and inhaled bronchodilators7 during exercise has been shown to improve exercise capacity and dyspnoea. Emphysema and hyperinflation have additionally been shown to have significant impacts on cardiovascular function.8
Additionally, systemic inflammation is prominent in COPD, leading to reductions in peripheral and respiratory (intercostal) muscle bulk and quality.9–11 Such loss of muscle mass in hospitalised patients with COPD independently predicts increased mortality and risk of hospital readmission.12
The parasternal intercostal muscles are purely inspiratory in nature, with contraction resulting in cranial and outward rib displacement.13 We have demonstrated that parasternal intercostal muscle thickness and quality in COPD have high intra- and inter-rater reliability when measured with ultrasound and that changes in these measures (decrease in thickness and increase in echogenicity) correlate with spirometric disease severity.14 The clinical significance of intercostal muscle function is an area of ongoing interest. Augmentation of intercostal blood flow and oxygen delivery in COPD with heliox during exercise results in reduced dyspnoea and respiratory muscle work,15,16 independent of redistribution of blood flow.17 Additionally, failure of EMG-parasternal intercostal muscle drive to improve following COPD exacerbation is associated with increased risk of hospital readmission,18 with elevated EMG activity associated with increased mortality.19 Finally, altered parasternal intercostal muscle morphology in critically ill patients undergoing mechanical ventilation with decreased intercostal and diaphragm thickness is associated with prolonged duration of ventilation and longer ICU stay.20 Parasternal intercostal thickening is increased in patients with diaphragm dysfunction, and this thickening is independently associated with failure of spontaneous breathing trials.21
A key finding in our previous study was that parasternal muscle thickness was reduced in more severe COPD; there are several potential reasons for this finding, which require further exploration. Firstly, there is an established relationship between muscle bulk and COPD severity which reflects the underlying inflammatory nature of COPD, along with general patient factors including reduced physical activity, comorbid medical conditions and medication use.22 Therefore, the reduction in muscle thickness may indicate a more generally unwell patient.
Another potential explanation for diminished parasternal intercostal muscle thickness in COPD is from muscle stretch due to thoracic hyperinflation. This may impair muscle length–tension relationships, and ─ in association with changes in rib displacement during contraction ─ reduce the capacity of parasternal intercostal muscles to effectively generate pressure changes at the airway level.23
In COPD, relief of hyperinflation can be achieved in selected patients via endobronchial valve (EBV) insertion. These one-way valves allow expiratory but prevent inspiratory flow, causing collapse of distal lung segments. These anatomical changes are usually accompanied by improvements in lung function, symptoms and exercise capacity.24–27
To establish ─ under clinical experimental conditions ─ whether hyperinflation contributes significantly to diminished parasternal muscle thickness in COPD, we performed ultrasound measurements before and after therapeutic unilateral EBV insertion to reverse hyperinflation. We aimed to assess whether there would be a demonstrable change in parasternal thickness in patients undergoing this procedure and to assess the relationship between physiological and radiological measures of lung volume and ultrasound indices.
We hypothesised that diminished parasternal intercostal muscle thickness on ultrasound would be reversed following EBV insertion, commensurate with the extent to which hyperinflation was successfully relieved.
Respiratory muscle ultrasound was performed on consecutive patients undergoing endobronchial valve placement at the Royal Melbourne Hospital, a university referral centre, prior to and following EBV insertion between 1 January 2017 and 30 June 2018. The baseline data of these patients have been included in a previously published work in a larger cohort.14
The decision to insert EBV was made on the basis of consensus guidelines,28 with patients demonstrating a residual volume (RV) of greater than 150%, with CT-measured fissural completeness, and Chartis® (Pulmonx, Redwood City, CA, USA) performed in the setting of equivocal scans to exclude collateral ventilation. Patients also underwent CT chest scanning post-procedure to assess changes in CT-measured total lung volume. No specific nutritional or physical interventions were performed between scans, of note pulmonary rehabilitation was not undertaken routinely for logistical reasons.
Baseline St George Respiratory Questionnaire (SGRQ),29 short-form 7-day recall International Physical Activity Questionnaire (IPAQ)30,31 and Charlson Comorbidity Index32,33 were obtained, along with routine physiological data (Table 1). Written informed consent was obtained from all patients prior to initial ultrasound with baseline data included in our previously published work,14 and this project received institutional ethics approval (Melbourne Health Human Research Ethics Committee: HREC 2015.296) and was conducted in accordance with the Declaration of Helsinki.
Table 1 Baseline Demographics
Ultrasound was performed by a single operator (PW) with 8 years of ultrasound experience and qualifications in respiratory ultrasound14,34 Briefly, B-mode images were obtained utilising a 6–14mHz linear array on a MindRay TE-5 machine (Shenzhen MindRay Bio-Medical Electronics Co. Ltd. Shenzen, China) with the participant at 45 degrees head-up in supine position on bed with standardised depth and gain.
Two still images were taken of the 2nd parasternal intercostal muscle bilaterally in the sagittal plane at end-tidal inspiration with a window visualising the 2nd/3rd ribs respectively (Figure 1) with the mean thickness of 2 measurements utilised for analysis; intercostal muscle echogenicity was analysed using histogram analysis. Additional musculoskeletal ultrasound measurements were taken, including right diaphragm thickness at end inspiration and expiration using B-mode and excursion measured with M-mode.35 The second parasternal intercostal muscle was chosen as this was the most reliable site in our previous work.14 Ultrasound was performed prior to EBV insertion, and approximately eight weeks following EBV insertion, at time of clinical review.
Measurements were performed on stored images using freely available NIH ImageJ software (https://imagej.nih.gov/ij/index.html NIH, Bethesda, MD, USA). Muscle thickness in millimetres was measured between the inner and outermost echogenic layer of the muscle fascial borders for both intercostal and diaphragm muscles. Muscle echogenicity was calculated utilising the square method36 to define the region of interest for analysis using ImageJ histogram function (Figure 1).
Data were analysed using GraphPad Prism 7 (GraphPad Software Inc., CA USA). Descriptive statistics were performed utilising parametric tests after testing for normality of distribution with D’Agostino and Pearson normality test. Correlation was measured using Pearson correlation coefficient. P-values were not reported for correlation data given the limited number of subjects. Data are described by mean and standard deviation unless otherwise specified, depending on normality of distribution.
Reporting is in keeping with STROBE guidelines.37
Eight consecutive patients who underwent EBV placement (Table 1) were included in this study. A ninth also underwent EBV insertion during this period however valves were removed prior to follow-up scanning due to progressive dyspnoea with respiratory failure.
In keeping with published data, there was a range of physiological responsiveness to EBV insertion in our cohort, with regards to airflow obstruction (FEV1), hyperinflation (RV) and quality of life (SGRQ) (Table 2).
Table 2 Pre- and Post-Physiological and QoL Results
Overall there was a non-significant increase in FEV1 and fall in RV likely due to the small sample size, with a magnitude of effect similar to published results.26 Unsurprisingly there was also no overall significant improvement in SGRQ (Table 2). There was a significant decrease in CT-measured volume of the treated lung (−611.6mL (95% CI −1145 to −78.73) p = 0.03), with a non-significant trend towards increased volume on the non-treated side (+181.6mL (95% CI −50.66 to 413.8mL) p = 0.107). Target lobe atelectasis was achieved in 3 patients, with significant decreases in target lobe volume (−0.887mL (95% CI −1370 to −404.2) p = 0.003).
Intercostal Muscle Changes
There was a strong negative correlation between change in residual volume and parasternal intercostal muscle thickness on the treated side in all participants (r = −0.883 p = 0.004). Additionally, there was a strong positive correlation between change in RV and 2nd parasternal thickness on the non-treated side (r = 0.840 p = 0.009) (Figure 2). The strength of correlation appeared stronger in those where there was target lobe atelectasis (r = −0.955 and r = 0.945, respectively).
There was a strong negative correlation between change in target lobe volume and change in parasternal intercostal thickness on the treated (r = −0.771 p = 0.025) side, with a strong positive correlation on the non-treated side (r = 0.755 p = 0.03) (Figure 2).
There was a non-significant correlation between change in overall CT-measured lung volume and parasternal intercostal thickness on the treated side (r = −0.517 p = 0.189) and between change in CT-volume and parasternal thickness on the non-treated side (r = 0.479 p 0.229), although the directionality of relationships was preserved. In those achieving target atelectasis, again there were strong correlation coefficients noted between change in CT-volume and parasternal thickness on the treated and non-treated sides (r = −0.999 and r = 0.975, respectively).
Overall, there was no significant correlation identified between change in parasternal thickness and change in FEV1 for either the treated or non-treated hemithorax (treated side r = 0.42 p = 0.295 and non-treated side r = −0.511 p = 0.195), and no significant relationship between change in CT volume and change in parasternal thickness for either side (treated side r = −0.599, p = 0.117 and non-treated side r = 0.398 p = 0.329).
There was no significant correlation identified between change in intercostal muscle echogenicity and change RV, FEV1, ipsilateral CT or total CT volume.
Diaphragm Muscle and Physical Activity Changes
There were non-significant moderate negative correlations between change in diaphragm thickness and excursion and change in RV (r = −0.662 p = 0.074 and −0.341 p = 0.409, respectively), with a moderate non-significant positive correlation between change in diaphragm thickness and change in FEV1 (r = 0.524 p = 0.183). There were similar results with regards to change in target lobe volume on CT for thickness (r = −0.659 p = 0.76), with no suggestion of a linear relationship between diaphragm excursion and change in target lobe volume (r = −0.067 p = 0.875).
There was no significant difference between pre-IPAQ raw scores or calculated metabolic equivalents per week and those following EBV insertion.
Changes in Intercostal Muscle Thickness
We have demonstrated a significant linear relationship between changes in intercostal muscle thickness and residual volume in patients who have undergone EBV. In the hemithorax treated with EBV insertion, relief of hyperinflation was accompanied by an increase in parasternal muscle thickness, exactly as we hypothesised.
Overall, these experimental results support the hypothesis that diminished parasternal muscle thickness in COPD is at least in significant part due to hyperinflation. This does not exclude the possibility that systemic inflammation and general muscle factors may also play important roles. Patients however had low levels of comorbidity as reflected in age-adjusted Charlson Comorbidity Index scores between 4 and 5, which reflected age and COPD status with no other recorded significant comorbidity.
The mechanism of benefit of endobronchial lung volume reduction is thought to be predominantly due to improved FEV1 following relief of hyperinflation. Limited studies have suggested other factors, including improved cardiac function,38 ventilation-perfusion matching39 and improved chest wall synchrony.40 Our findings suggest for the first time that improved intercostal muscle function may also contribute to symptomatic improvements. Increased parasternal muscle thickness following EBV insertion is likely accompanied by a restoration of optimal length–tension relationships and improved rib conformation.
It has previously been demonstrated that hyperinflation reduces the ability of both the diaphragm and parasternal intercostal muscles to alter airway opening pressure in a volume-dependent fashion23 with a relative increase in the contribution to negative pressure generation by the intercostal muscles with progressive hyperinflation.41 Our results provide a second mechanistic explanation for the contribution of intercostal muscles toward clinical benefits seen in lung volume reduction.
In the contralateral hemithorax (ie not treated with EBV insertion), we found an unexpected reduction in intercostal muscle thickness, possibly suggesting a reciprocal relationship between changes in rib conformation in each hemithorax in response to increased volume of the contralateral lung. Interestingly, in the study by Zoumot et al40 chest wall synchrony was only significantly improved on the treated hemithorax, which is also in keeping with our findings. This lends further support towards the benefit of optimising muscle length–tension relationships and alteration in the direction of rib displacement related to the interaction between rib displacement and lung volume to improve function.23
The correlations identified between parasternal muscle thickness and changes in RV and target lobe volume were stronger than for changes in diaphragm indices; nevertheless, the relative contributions of each of these muscle groups require further evaluation.
The demonstration of a strong linear relationship between parasternal intercostal thickness and residual volume suggests benefit for ultrasound measurement as a simple, safe and repeatable bedside marker mirroring changes in hyperinflation and gas trapping. Additionally, the demonstration of strong relationships between target lobe volume change and parasternal thickness may allow rapid assessment of changes in response to EBV insertion if replicated in larger cohorts. The greater strength of correlation in those achieving target lobe atelectasis further underlines these results.
Changes in CT Lung Volume
We have demonstrated significant correlations between changes in parasternal thickness and changes in target lobe volume, reflecting the responsiveness of this measure to relief of hyperinflation.
CT-defined lung volume did not show the same strength of correlation with ultrasound indices as physiological measurement of residual volume or target lobe volume changes, although the direction of correlations was consistent. CT scans were performed at deep inspiration which has previously been shown to underestimate TLC by approximately 12%, with expiratory scans (not performed on this occasion) significantly overestimating RV.42 We hypothesise that the non-significant correlation is related to the small sample size, as well as the inherent variability of CT volume measurements related to the lack of standardisation of inspiration. This is supported the fact there was no significant correlation between change in overall CT volume and change in either TLC (r = 0.344 p 0.404) or RV (r = 0.682 p = 0.062) in our dataset.
Changes in Intercostal Muscle Echogenicity
We did not observe changes in muscle echogenicity, though the study period is perhaps shorter than required to observed changes in the composition of these small muscles. It is worth noting that in the context of critical illness,43 there are demonstrable changes in echogenicity within larger muscle groups within 10 days, although this is a highly catabolic state. Studies repeating respiratory muscle ultrasound assessment at more distant time points will be required to assess the impact of EBV on muscle quality.
Changes in Diaphragm Thickness and Excursion
There was no significant difference between measurements in either diaphragm thickness or excursion pre- and post-EBV insertion. There was a non-significant negative correlation between changes in RV, target lobe volume and diaphragm thickness. The direction of this relationship was as anticipated based on the relationship between the diaphragm and hyperinflation, with previous studies showing alterations in the diaphragm zone of apposition on US in hyperinflation.44,45 A larger cohort may have identified a significant relationship between these parameters.
Change in diaphragm excursion showed a non-significant correlation with change in RV and target lobe volumes, which is as would be anticipated given the improvements given previous studies using MRI46 and US47 showing reduced excursion with hyperinflation with improvement following lung volume reduction surgery.48
There are limitations to our data. Firstly, this is a small study, with scans performed by a single operator. Whilst we have previously shown excellent intra-observer agreement including with these pre-EBV data in our previously,14 the reproducibility of these findings by other sonographers is needed. Secondly, although endobronchial valve insertion affords an experimental opportunity to study parasternal muscles following a defined physiological intervention – relief of hyperinflation – it remains to be shown whether such changes occur following other therapeutic interventions. Finally, the addition of a control group of patients with COPD who have not undergone intervention would add further strength to these findings.
These data raise a possible mechanism by which EBV insertion may improve symptoms, via the alterations in intercostal muscles, likely due to restoration of optimal length–tension relationships. Measurements of intercostal thickness may provide clinicians with information regards to hyperinflation, particularly with regards to changes over time, with increasing thickness strongly related to reducing RV. This may provide clinicians with a non-invasive easily performed marker at the bedside.
The emerging ICU data with regards to intercostal muscle thickness/thickening and ventilation outcomes are of interest,21 raising the possibility that adding intercostal measurements to diaphragm US may help identify those who are at risk of difficulties with ventilation weaning. Our data further underline the value of ultrasound imaging of the parasternal muscles.
Finally, a larger cohort study utilising controls who do not undergo mechanical relief of hyperinflation would provide further data with regards to the relationship between lung volume and intercostal muscle thickness. A longer duration of follow-up would allow further changes to be described, including changes in echogenicity which were likely not identified in our study due to the limited time between scans. These data would be further enriched by measurement of serum inflammatory markers, parasternal muscle EMG and ideally muscle biopsies to assess for remodelling, with previous research suggesting that in severe COPD parasternal muscles undergo fibre switching to fast to slow fibre and slow myosin heavy chain types.49 This would also help further elucidate the relationship with diaphragm parameters, with our small sample size precluding any firm conclusions. Measurements of respiratory muscle strength, particularly with regards to inspiratory pressure generation would be of interest.
Dynamic changes in parasternal intercostal muscle thickness can be observed on respiratory muscle ultrasound. In patients with COPD undergoing EBV insertion, parasternal intercostal muscle thickness increased in proportion to the relief of hyperinflation, providing a mechanistic explanation for the diminished muscle thickness observed in COPD.
These findings also provide a further mechanism of symptomatic benefit following bronchoscopic lung volume reduction with EBV insertion.
COPD, chronic obstructive pulmonary disease; CT, computed tomography; EBV, endobronchial valve; IPAQ, International Physical Activity Questionnaire; FEV1, forced expiratory volume in 1 second; RV, residual volume; SGRQ, St George Respiratory Questionnaire; TLC, total lung capacity.
We gratefully acknowledge the invaluable assistance of Michael Hofmann and Jason Callahan from the Department of Molecular Imaging, Peter McCallum Cancer Centre, Parkville Australia in the acquisition of lung volume data. The abstract of this paper was presented at the 2018 ERS International Conference as a poster presentation with interim findings. The poster’s abstract was published in “Poster Abstracts” in European Respiratory Journal 2018 52: PA5399; DOI: 10.1183/13993003.congress-2018.PA5399.
Dr Selina M Parry reports grants from NHMRC Early Career Fellowship, outside the submitted work. The authors report no other conflicts of interest in this work.
1. Vogelmeier CF, Criner GJ, Martinez FJ, et al. Global strategy for the diagnosis, management and prevention of chronic obstructive lung disease 2017 report: GOLD executive summary. Respirology. 2017;22(3):575–601.
2. Ofir D, Laveneziana P, Webb KA, Lam YM, O’Donnell DE. Mechanisms of dyspnea during cycle exercise in symptomatic patients with GOLD stage I chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2008;177(6):622–629. doi:10.1164/rccm.200707-1064OC
3. Guenette JA, Webb KA, O’Donnell DE. Does dynamic hyperinflation contribute to dyspnoea during exercise in patients with COPD? Eur Respir J. 2012;40(2):322–329. doi:10.1183/09031936.00157711
4. Laveneziana P, Webb KA, Ora J, Wadell K, O’Donnell DE. Evolution of dyspnea during exercise in chronic obstructive pulmonary disease: impact of critical volume constraints. Am J Respir Crit Care Med. 2011;184(12):1367–1373. doi:10.1164/rccm.201106-1128OC
5. De Troyer A, Leeper JB, McKenzie DK, Gandevia SC. Neural drive to the diaphragm in patients with severe COPD. Am J Respir Crit Care Med. 1997;155(4):1335–1340. doi:10.1164/ajrccm.155.4.9105076
6. Palange P, Valli G, Onorati P, et al. Effect of heliox on lung dynamic hyperinflation, dyspnea, and exercise endurance capacity in COPD patients. J Appl Physiol (1985). 2004;97(5):1637–1642. doi:10.1152/japplphysiol.01207.2003
7. O’Donnell DE, Webb KA, Neder JA. Lung hyperinflation in COPD: applying physiology to clinical practice. COPD Res Pract. 2015;1(1):4. doi:10.1186/s40749-015-0008-8
8. Barr RG, Bluemke DA, Ahmed FS, et al. Percent emphysema, airflow obstruction, and impaired left ventricular filling. N Engl J Med. 2010;362(3):217–227. doi:10.1056/NEJMoa0808836
9. Seymour JM, Spruit MA, Hopkinson NS, et al. The prevalence of quadriceps weakness in COPD and the relationship with disease severity. Eur Respir J. 2010;36(1):81–88. doi:10.1183/09031936.00104909
10. Guerri R, Gayete A, Balcells E, et al. Mass of intercostal muscles associates with risk of multiple exacerbations in COPD. Respir Med. 2010;104(3):378–388. doi:10.1016/j.rmed.2009.10.015
11. Park MJ, Cho JM, Jeon KN, et al. Mass and fat infiltration of intercostal muscles measured by CT histogram analysis and their correlations with COPD severity. Acad Radiol. 2014;21(6):711–717. doi:10.1016/j.acra.2014.02.003
12. Greening NJ, Harvey-Dunstan TC, Chaplin EJ, et al. Bedside assessment of quadriceps muscle by ultrasound after admission for acute exacerbations of chronic respiratory disease. Am J Respir Crit Care Med. 2015;192(7):810–816. doi:10.1164/rccm.201503-0535OC
13. De Troyer A, Kirkwood PA, Wilson TA. Respiratory action of the intercostal muscles. Physiol Rev. 2005;85(2):717–756. doi:10.1152/physrev.00007.2004
14. Wallbridge P, Parry SM, Das S, et al. Parasternal intercostal muscle ultrasound in chronic obstructive pulmonary disease correlates with spirometric severity. Sci Rep. 2018;8(1):15274. doi:10.1038/s41598-018-33666-7
15. Vogiatzis I, Habazettl H, Aliverti A, et al. Effect of helium breathing on intercostal and quadriceps muscle blood flow during exercise in COPD patients. Am J Physiol Regul Integr Comp Physiol. 2011;300(6):R1549–59. doi:10.1152/ajpregu.00671.2010
16. Louvaris Z, Vogiatzis I. Contrasting the physiological effects of heliox and oxygen during exercise in a patient with advanced COPD. Breathe (Sheff). 2019;15(3):250–257. doi:10.1183/20734735.0197-2019
17. Louvaris Z, Vogiatzis I, Aliverti A, et al. Blood flow does not redistribute from respiratory to leg muscles during exercise breathing heliox or oxygen in COPD. J Appl Physiol (1985). 2014;117(3):267–276. doi:10.1152/japplphysiol.00490.2014
18. Suh ES, Mandal S, Harding R, et al. Neural respiratory drive predicts clinical deterioration and safe discharge in exacerbations of COPD. Thorax. 2015;70(12):1123–1130. doi:10.1136/thoraxjnl-2015-207188
19. Patout M, Meira L, D’Cruz R, et al. Neural respiratory drive predicts long-term outcome following admission for exacerbation of COPD: a post hoc analysis. Thorax. 2019;74(9):910–913. doi:10.1136/thoraxjnl-2018-212074
20. Nakanishi N, Oto J, Ueno Y, Nakataki E, Itagaki T, Nishimura M. Change in diaphragm and intercostal muscle thickness in mechanically ventilated patients: a prospective observational ultrasonography study. J Intensive Care. 2019;7:56. doi:10.1186/s40560-019-0410-4
21. Dres M, Dube BP, Goligher E, et al. Usefulness of parasternal intercostal muscle ultrasound during weaning from mechanical ventilation. Anesthesiology. 2020;132(5):1114–1125. doi:10.1097/ALN.0000000000003191
22. Maltais F, Decramer M, Casaburi R, et al. An official American Thoracic Society/European Respiratory Society statement: update on limb muscle dysfunction in chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 2014;189(9):e15–62. doi:10.1164/rccm.201402-0373ST
23. De Troyer A, Wilson TA. Effect of acute inflation on the mechanics of the inspiratory muscles. J Appl Physiol (1985). 2009;107(1):315–323. doi:10.1152/japplphysiol.91472.2008
24. Klooster K, Slebos DJ, Zoumot Z, Davey C, Shah PL, Hopkinson NS. Endobronchial valves for emphysema: an individual patient-level reanalysis of randomised controlled trials. BMJ Open Respir Res. 2017;4(1):e000214. doi:10.1136/bmjresp-2017-000214
25. Criner GJ, Sue R, Wright S, et al. A Multicenter RCT of Zephyr(R) endobronchial valve treatment in heterogeneous emphysema (LIBERATE). Am J Respir Crit Care Med. 2018;198(9):1151–1164. doi:10.1164/rccm.201803-0590OC
26. Davey C, Zoumot Z, Jordan S, et al. Bronchoscopic lung volume reduction with endobronchial valves for patients with heterogeneous emphysema and intact interlobar fissures (the BeLieVeR-HIFi trial): study design and rationale. Thorax. 2015;70(3):288–290. doi:10.1136/thoraxjnl-2014-205127
27. Park TS, Hong Y, Lee JS, et al. Bronchoscopic lung volume reduction by endobronchial valve in advanced emphysema: the first Asian report. Int J Chron Obstruct Pulmon Dis. 2015;10:1501–1511.
28. Slebos DJ, Shah PL, Herth FJ, Valipour A. Endobronchial valves for endoscopic lung volume reduction: best practice recommendations from expert panel on endoscopic lung volume reduction. Respir Int Rev Thor Dis. 2017;93(2):138–150. doi:10.1159/000453588
29. Jones PW, Quirk FH, Baveystock CM, Littlejohns P. A self-complete measure of health status for chronic airflow limitation. The St George’s Respiratory Questionnaire. Am Rev Respir Dis. 1992;145(6):1321–1327. doi:10.1164/ajrccm/145.6.1321
30. Craig CL, Marshall AL, Sjostrom M, et al. International physical activity questionnaire: 12-country reliability and validity. Med Sci Sports Exerc. 2003;35(8):1381–1395. doi:10.1249/01.MSS.0000078924.61453.FB
31. Hurtig-Wennlof A, Hagstromer M, Olsson LA. The International Physical Activity Questionnaire modified for the elderly: aspects of validity and feasibility. Public Health Nutr. 2010;13(11):1847–1854. doi:10.1017/S1368980010000157
32. Charlson M, Szatrowski TP, Peterson J, Gold J. Validation of a combined comorbidity index. J Clin Epidemiol. 1994;47(11):1245–1251. doi:10.1016/0895-4356(94)90129-5
33. Hall WH, Ramachandran R, Narayan S, Jani AB, Vijayakumar S. An electronic application for rapidly calculating Charlson comorbidity score. BMC Cancer. 2004;4:94. doi:10.1186/1471-2407-4-94
34. Wallbridge PD, Joosten SA, Hannan LM, et al. A prospective cohort study of thoracic ultrasound in acute respiratory failure: the C3PO protocol. JRSM Open. 2017;8(5):2054270417695055. doi:10.1177/2054270417695055
35. Sarwal A, Walker FO, Cartwright MS. Neuromuscular ultrasound for evaluation of the diaphragm. Muscle Nerve. 2013;47(3):319–329. doi:10.1002/mus.23671
36. Sarwal A, Parry SM, Berry MJ, et al. Interobserver reliability of quantitative muscle sonographic analysis in the critically Ill population. J Ultrasound Med. 2015;34(7):1191–1200. doi:10.7863/ultra.34.7.1191
37. von Elm E, Altman DG, Egger M, et al. The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement: guidelines for reporting observational studies. PLoS Med. 2007;4(10):e296. doi:10.1371/journal.pmed.0040296
38. Pizarro C, Schueler R, Hammerstingl C, Tuleta I, Nickenig G, Skowasch D. Impact of endoscopic lung volume reduction on right ventricular myocardial function. PLoS One. 2015;10(4):e0121377. doi:10.1371/journal.pone.0121377
39. Pizarro C, Ahmadzadehfar H, Essler M, et al. Effect of endobronchial valve therapy on pulmonary perfusion and ventilation distribution. PLoS One. 2015;10(3):e0118976. doi:10.1371/journal.pone.0118976
40. Zoumot Z, LoMauro A, Aliverti A, et al. Lung volume reduction in emphysema improves chest wall asynchrony. Chest. 2015;148(1):185–195. doi:10.1378/chest.14-2380
41. Martinez FJ, Couser JI, Celli BR. Factors influencing ventilatory muscle recruitment in patients with chronic airflow obstruction. Am Rev Respir Dis. 1990;142(2):276–282. doi:10.1164/ajrccm/142.2.276
42. Kauczor HU, Heussel CP, Fischer B, Klamm R, Mildenberger P, Thelen M. Assessment of lung volumes using helical CT at inspiration and expiration: comparison with pulmonary function tests. AJR Am J Roentgenol. 1998;171(4):1091–1095. doi:10.2214/ajr.171.4.9763003
43. Parry SM, El-Ansary D, Cartwright MS, et al. Ultrasonography in the intensive care setting can be used to detect changes in the quality and quantity of muscle and is related to muscle strength and function. J Crit Care. 2015;30(5):
44. Gorman RB, McKenzie DK, Pride NB, Tolman JF, Gandevia SC. Diaphragm length during tidal breathing in patients with chronic obstructive pulmonary disease. American Journal of Respiratory & Critical Care Medicine. 2002;166(11):1461–1469. doi:10.1164/rccm.200111-087OC
45. Gorman RB, McKenzie DK, Butler JE, Tolman JF, Gandevia SC. Diaphragm length and neural drive after lung volume reduction surgery. American Journal of Respiratory & Critical Care Medicine. 2005;172(10):1259–1266. doi:10.1164/rccm.200412-1695OC
46. Iwasawa T, Kagei S, Gotoh T, et al. Magnetic resonance analysis of abnormal diaphragmatic motion in patients with emphysema. Eur Respir J. 2002;19(2):225–231. doi:10.1183/09031936.02.00044602
47. Baria MR, Shahgholi L, Sorenson EJ, et al. B-mode ultrasound assessment of diaphragm structure and function in patients with COPD. Chest. 2014;146(3):680–685. doi:10.1378/chest.13-2306
48. Suga K, Tsukuda T, Awaya H, et al. Impaired respiratory mechanics in pulmonary emphysema: evaluation with dynamic breathing MRI. J Magn Resonance Imaging. 1999;10(4):510–520. doi:10.1002/(SICI)1522-2586(199910)10:4<510::AID-JMRI3>3.0.CO;2-G
49. Levine S, Nguyen T, Friscia M, et al. Parasternal intercostal muscle remodeling in severe chronic obstructive pulmonary disease. J Appl Physiol (1985). 2006;101(5):1297–1302. doi:10.1152/japplphysiol.01607.2005