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Prevalence of Aminoglycoside Resistance and Aminoglycoside Modifying Enzymes in Acinetobacter baumannii Among Intensive Care Unit Patients, Ismailia, Egypt

Authors Kishk R, Soliman N, Nemr N, Eldesouki R, Mahrous N, Gobouri A, Azab E, Anani M

Received 14 November 2020

Accepted for publication 18 December 2020

Published 19 January 2021 Volume 2021:14 Pages 143—150

DOI https://doi.org/10.2147/IDR.S290584

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Suresh Antony


Rania Kishk,1 Nourhan Soliman,2 Nader Nemr,3 Raghda Eldesouki,4 Nageh Mahrous,3 Adil Gobouri,5 Ehab Azab,6 Maha Anani2

1Microbiology and Immunology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt; 2Clinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt; 3Endemic and Infectious Diseases Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt; 4Genetics Unit, Histology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt; 5Department of Chemistry, College of Science, Taif University, Taif 21944, Saudi Arabia; 6Department of Biotechnology, College of Science, Taif University, Taif 21944, Saudi Arabia

Correspondence: Rania Kishk
Faculty of Medicine, Suez Canal University, Ismailia, Egypt
Tel +201025099921
Email rankishk@med.suez.edu.eg

Background: Acinetobacter baumannii is an opportunistic pathogen that rapidly develops antibiotic resistance against commonly prescribed antimicrobial agents in hospitalized patients worldwide. Aminoglycosides are commonly used in the treatment of A. baumannii health care-associated infections (HAIs). Aminoglycosides resistance mechanisms are varied and commonly involve production of aminoglycoside-modifying enzymes (AME) and efflux systems.
Aim: This study aimed to provide an insight into the frequency of genes encoding AME in A. baumannii strains isolated from different clinical specimens in intensive care units (ICU).
Methodology: A total of 52 multidrug-resistant (MDR) A. baumannii strains were isolated from ICU, Suez Canal University Hospitals. Species identification and antibiotics susceptibility testing were done by the automated system VITEK 2. The genes encoding AME were detected by PCR.
Results: Aminoglycosides resistance (amikacin, gentamicin and tobramycin) was observed in 35 isolates (67.3%). We found that aacC1 gene was the predominant AME resistance gene among A. baumannii isolates, detected in 14 isolates (40%), aphA6 in 11 isolates (31.4%) and addA1 in 5 isolates (14.2%). We found 5 isolates containing 2 AME genes, 3 of them with aacC1 and aphA6 and the remaining 2 with both aacC1 and aadA1 genes. Nearly, 5 isolates (14.2%) were negative for all AME resistance genes.
Conclusion: Our study indicated that AME encoding genes are predominant in A. baumannii strains in our region which stressed on the importance of preventive measures to control spreading of resistance genes.

Keywords: Acinetobacter baumannii, aminoglycoside-modifying enzymes; AME, aacC1, aphA6, aadA1, aadB

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