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Plasmid-Encoded blaNDM-5 Gene That Confers High-Level Carbapenem Resistance in Salmonella Typhimurium of Pork Origin

Authors Gao Y, Wen J, Wang S, Xu X, Zhan Z, Chen Z, Bai J, Qu X, Zhang H, Zhang J, Liao M

Received 11 February 2020

Accepted for publication 28 April 2020

Published 19 May 2020 Volume 2020:13 Pages 1485—1490

DOI https://doi.org/10.2147/IDR.S249357

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Sahil Khanna


Yuan Gao,1 Junping Wen,1 Shaojun Wang,1 Xuebin Xu,2 Zeqiang Zhan,1 Zhengquan Chen,1 Jie Bai,1 Xiaoyun Qu,1 Hongxia Zhang,1 Jianmin Zhang,1 Ming Liao1

1National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Zoonoses, Ministry of Agriculture, Key Laboratory of Animal Vaccine Development, Key Laboratory of Zoonoses Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, People’s Republic of China; 2Department of Microbiology, Shanghai Municipal Centre for Disease Control and Prevention, Shanghai, People’s Republic of China

Correspondence: Ming Liao; Jianmin Zhang
National and Regional Joint Engineering Laboratory for Medicament of Zoonoses Prevention and Control, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Zoonoses, Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong Province, College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou 510642, Guangdong, People’s Republic of China
Tel +86 020 85290240; +86 020 85280240
Fax +86 020 85290240; +86 020 85285282
Email mliao@scau.edu.cn; junfeng-v@163.com

Purpose: Carbapenem resistance is rarely reported in Salmonella Typhimurium, especially from a food origin. Here, we report a plasmid-mediated mobile carbapenem-resistant blaNDM-5 gene in Salmonella Typhimurium isolated from pork in Shanghai, China in 2016.
Patients and Methods: In July 2016, the S. Typhimurium SH160 strain was recovered from minced pork meat purchased from a supermarket in Yangpu District, Shanghai, China. Antimicrobial susceptibility testing, multi-locus sequence typing, conjugation, S1-PFGE, southern hybridization, whole-genome sequencing and data analysis were performed.
Results: This isolate was found to be a ST34 strain and resistant to carbapenems, cephalosporins, and most other commonly used antibiotics. The blaNDM-5 gene was harbored by a 46161-bp IncX3 plasmid which was found to be transferable. The IncX3 plasmid contains a composite cassette, consisting of ISSwil-IS3000-ΔISAba125-IS5-blaNDM-5-bleMBL-trpF-dsbC-IS26-ctuA1-ΔumuD. In addition, this strain was found to harbor an additional 161706-bp IncHI2 plasmid which carries nine resistant genes, such as aadA1, aadA3, aph(3ʹ)-la, sul1, sul2, sul3, floR, cmlA and dfrA12.
Conclusion: We reported the S. Typhimurium with transferable IncX3 plasmid harboring blaNDM-5 gene from minced pork. We characterized the complete genetic features of the plasmid, which demonstrated the potential for spreading in different bacterial pathogens. Therefore, extensive surveillance and monitoring for carbapenem-resistant bacterium in the food chain and public health are urgently required.

Keywords: S. Typhimurium, pork, blaNDM-5, public health

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