Back to Journals » Cancer Management and Research » Volume 10

Overexpression of chloride channel-3 predicts unfavorable prognosis and promotes cellular invasion in gastric cancer

Authors Peng J, Chen W, Chen J, Yuan Y, Zhang J, He Y

Received 13 December 2017

Accepted for publication 14 March 2018

Published 14 May 2018 Volume 2018:10 Pages 1163—1175


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Leylah Drusbosky

Jianjun Peng, Wei Chen, Jianhui Chen, Yujie Yuan, Jian Zhang, Yulong He

Gastrointestinal Surgical Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China

Background: Chloride channel-3 (CLC-3) has been reported to promote the proliferation and invasion in various tumors, yet little is known about its role in gastric cancer. In the present study, we investigated the clinical significance of CLC-3 and its biological role in gastric cancer.
Methods: Bioinformatic analysis, immunohistochemical staining, quantitative real-time polymerase chain reaction and Western blot assay were used to assess the expression of CLC-3 and its clinical significance in gastric cancer. The biological role of CLC-3 and its underlying mechanism were detected through in vitro experiments.
Results: CLC-3 was highly expressed in gastric cancer tissues and cell lines, and high levels of CLC-3 were significantly associated with adverse clinicopathological parameters and shorter overall survival time in patients with gastric cancer. Functional studies revealed that silencing of CLC-3 decreased, while overexpression promoted, the proliferation, migration and invasion of gastric cancer cells in vitro. Mechanistic studies suggested that canonical TGF-β/Smad signaling pathway is involved in CLC-3-induced gastric cancer cells proliferation, migration and invasion.
Conclusion: These findings indicate the vital role of CLC-3 in gastric cancer progression and its potential role of a therapeutic target for treatment.

Keywords: CLC-3, gastric cancer, proliferation, migration, prognosis, TGF-β

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]