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Overexpression of BUB1B contributes to progression of prostate cancer and predicts poor outcome in patients with prostate cancer

Authors Fu X, Chen G, Cai Z, Wang C, Liu Z, Lin Z, Wu Y, Liang Y, Han Z, Liu J, Zhong W

Received 7 December 2015

Accepted for publication 22 February 2016

Published 15 April 2016 Volume 2016:9 Pages 2211—2220

DOI https://doi.org/10.2147/OTT.S101994

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Manfred Beleut

Peer reviewer comments 2

Editor who approved publication: Dr Jianmin Xu


Xin Fu,1 Guo Chen,1 Zhi-duan Cai,1,2 Cong Wang,3 Ze-zhen Liu,1 Zhuo-yuan Lin,1,2 Yong-ding Wu,1,4 Yu-xiang Liang,1 Zhao-dong Han,1 Jun-chen Liu,5 Wei-De Zhong,1,2,6

1Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People’s Hospital, Guangzhou Medical University, 2Guangdong Provincial Institute of Nephrology, Nanfang Hospital, Southern Medical University, 3School of Pharmacy, Wenzhou Medical University, Wenzhou, 4School of Public Health, Guangzhou Medical University, Guangzhou, People’s Republic of China; 5Center for Translational Cancer Research, Institute of Biosciences and Technology and College of Medicine, Texas A&M Health Science Center, Houston, TX, USA; 6Urology Key Laboratory of Guangdong Province, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, People’s Republic of China

Abstract: BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B) is a member of the spindle assembly checkpoint protein family, which has been proven to be associated with many kinds of cancers. The aim of this study was to investigate whether BUB1B was correlated with progression and prognosis in patients with prostate cancer (PCa) and how BUB1B regulated the proliferation, migration, and invasion of PCa cell lines. Compared to benign prostate cells and tissues, both messenger RNA and protein expressions of BUB1B were statistically increased in PCa cell lines and tumor tissues. In vitro studies revealed that BUB1B overexpression enhanced the proliferation, migration, and invasion ability of PCa cell lines, whereas depletion of BUB1B did not affect the cell functions. Microarray analysis showed the positive staining of BUB1B was upregulated in the higher Gleason score group, which also correlated with advanced clinicopathological stage, higher serum prostate-specific antigen, metastasis, overall survival, and prostate-specific antigen failure. Furthermore, the survival analysis indicated that high expression of BUB1B was an independent predictor for shorter biochemical recurrence-free survival, which had no effect on overall survival. BUB1B plays an important role in tumor growth and progression, which can lead to its use as a potential biomarker for the diagnosis and prognosis of PCa.

Keywords: prostate cancer, BUB1B, SAC, biochemical recurrence-free survival, prognosis
 

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