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Osthole sensitizes with radiotherapy to suppress tumorigenesis of human nasopharyngeal carcinoma in vitro and in vivo

Authors Peng L, Huang YT, Chen J, Zhuang YX, Zhang F, Chen JY, Zhou L, Zhang DH

Received 6 August 2018

Accepted for publication 6 October 2018

Published 8 November 2018 Volume 2018:10 Pages 5471—5477

DOI https://doi.org/10.2147/CMAR.S182798

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 2

Editor who approved publication: Dr Ahmet Emre Eskazan


Lin Peng1,*, Yi-Teng Huang2,*, Jian Chen3, Yi-Xuan Zhuang3, Fan Zhang4, Jiong-Yu Chen4, Li Zhou5, Dong-Hong Zhang6

1
Clinical Laboratory, Cancer Hospital of Shantou University Medical College, Shantou 515031, People’s Republic of China; 2Health Care Center, The First Affiliated Hospital of Shantou University Medical College. Shantou 515041, People’s Republic of China; 3Department of Radiotherapy, Cancer Hospital of Shantou University Medical College, Shantou 515031, People’s Republic of China; 4Oncological Research Lab, Cancer Hospital of Shantou University Medical College, Shantou 515031, People’s Republic of China; 5Department of Gynecological Oncology, Cancer Hospital of Shantou University Medical College, Shantou 515031, People’s Repulic of China; 6Department of Cardiology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang, People’s Repulic of China

*These authors contributed equally to this work

Background: Radiotherapy is one of the most comment and useful treatment for nasopharyngeal carcinoma (NPC), but the radioresistance remains a major obstacle. Osthole, a natural coumarin derivative, has been shown to have anti-tumor and anti-inflammatory activity. However, the relationship between osthole and NPC treatment, especially for radiotherapy, is still elusive.
Methods: Osthole with or without X ray radiotherapy treated with CNE2 cells, a human EC cell line. Cell viability, proliferation, migration and apoptosis were measured by MTT, colony formation, Annexin V/PI double staining, Transwell assay, respectively. NPC tumor models were established on BALB/c nude mice by subcutaneously injection of CNE2 cells and the effect of osthole and radiotherapy on tumor growth in vivo was studied.
Results: We found that in a dose-dependent manner, osthole could individually, and synergistically with radiotherapy, reduce NPC cell (CNE2) viability, proliferation, migration, and invasion, and induce apoptosis, respectively. This effect of anti-tumor growth and induction of apoptosis was further confirmed in mice induced by subcutaneously injection with CNE2 cells and following treated with osthole or/and radiation.
Conclusion: Osthole increases the effect of radiotherapy on anti-human nasopharyngeal cancer.
 
Keywords: osthole, radiotherapy, human nasopharyngeal carcinoma, tumorigenesis, proliferation, apoptosis

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