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Open field and a behavior score in PNT model for neuropathic pain in pigs

Authors Castel D, Sabbag I, Nasaev E, Peng S, Meilin S

Received 25 April 2018

Accepted for publication 28 June 2018

Published 11 October 2018 Volume 2018:11 Pages 2279—2293

DOI https://doi.org/10.2147/JPR.S172300

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Ms Justinn Cochran

Peer reviewer comments 3

Editor who approved publication: Dr Michael E Schatman


David Castel,1 Itai Sabbag,2 Elvira Nasaev,3 Sean Peng,4 Sigal Meilin3

1The Neufeld Cardiac Research Institute, Sheba Medical Centre, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel; 2Lahav Research Institute, Kibutz Lahav, Negev, Israel; 3Neurology R&D Division, MD Biosciences, Ness Ziona, Israel; 4Touchstone Biosciences, Plymouth Meeting, PA, USA

Background: Rodent models are frequently used in the research of pain and continue to provide valuable data on the mechanisms driving pain, although they are criticized due to limited translational ability to human conditions. Previously we have suggested pigs as a model for development of drugs for neuropathic pain. In this study, we investigate the spontaneous behavior of pigs following peripheral neuritis trauma (PNT)-induced neuropathic pain.
Methods: A computerized monitoring system was used to evaluate the changes in open field test in addition to applying a composite behavior scoring system. The data suggest that the PNT operation did not affect the animal’s ability to walk as the total distance walked by PNT animals was not significantly different from the total distance walked by sham-operated animals. However, PNT animals expressed a significant change in the pattern of walking. This effect was unrelated to the time that the animals spent in the open field. Following treatment with different drugs (morphine, buprenorphine, or gabapentin), the walking pattern of the animals in the open field changed in a drug-specific manner. In addition, the detailed behavior score revealed drug-specific changes following treatment.
Results: Pharmacokinetic analysis of the drug concentration in blood and cerebrospinal fluid correlated with the behavioral analysis.
Conclusion: The data of this study suggest that the open field test together with the detailed behavior score applied in this model are a powerful tool to assess the spontaneous behavior of pigs following PNT-induced neuropathic pain.

Keywords: open field, swine, neuropathic pain

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