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Neuropilin-1 is associated with the prognosis of cervical cancer in Henan Chinese population

Authors Yang L, Liu L, Zhu YH, Wang BB, Chen YN, Zhang F, Zhang XA, Ren CC

Received 13 November 2018

Accepted for publication 6 March 2019

Published 17 April 2019 Volume 2019:12 Pages 2911—2920

DOI https://doi.org/10.2147/OTT.S194349

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Andrew Yee

Peer reviewer comments 2

Editor who approved publication: Dr Leo Jen-Liang Su


Li Yang,1* Ling Liu,1* Yuan-Hang Zhu,1 Bing-Bing Wang,2 Yan-Nan Chen,1 Feng Zhang,1 Xiao-An Zhang,3 Chen-Chen Ren1

1Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, People’s Republic of China; 2Department of Obstetrics and Gynecology, Yuebei People’s Hosptial, Shaoguan 512025, People’s Republic of China; 3Department of Imaging, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, People’s Republic of China
 
*These authors contributed equally to this work
 
Objective: Neuropilin-1 has been reported to be a valuable diagnostic biomarker in patients with cervical intraepithelial neoplasia (CIN) and early cervical cancer. The aim of this study was to investigate the association between Neuropilin-1 and the prognosis of cervical cancer in Henan Chinese population.
Methods: Tissues were collected in The Third Affiliated Hospital of Zhengzhou University between 2010 and 2012, determining the level and expression of Neuropilin-1 in different cervical lesions by immunohistochemistry. The cell proliferation assay, wound-healing assays and Transwell assay were performed to explore the ability of proliferation, migration and invasion for Hela and Caski cells after NRP-1 was knocked down by shRNA transfection. Western blotting was performed to investigate the role of NRP-1 in endothelial-to-mesenchymal transition (EndMT). Tumor xenografts model was used to evaluate the effect of NRP-1 on the tumor growth.
Results: The expression of NRP-1 was upregulated in the tumor tissues compared with the CIN and normal tissues (P<0.0001). The overall survival time of the high NRP-1 expression group was significantly shorter than that of the low NRP-1 expression group (P<0.0001); NRP-1-depleted cells had dramatically lower rate of proliferation, migration and invasion compared to control cells (all P<0.05). Depletion of NRP-1 significantly suppressed the growth of CaSki xenograft tumor in nude mice.
Conclusions: The current study demonstrated that NRP-1 expression is significantly correlated with the progression of CC. Notably, high NRP-1 expression is correlated with a poorer survival in patients with CC, and has been shown to be an independent prognostic factor.

Keywords: Neuropilin-1, NRP-1, cervical cancer, EMT, proliferation

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