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Nanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property

Authors Alizadeh A, Razmjou A, Ghaedi M, Jannesar R

Received 4 October 2018

Accepted for publication 29 January 2019

Published 5 March 2019 Volume 2019:14 Pages 1669—1685

DOI https://doi.org/10.2147/IJN.S189728

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Anderson Oliveira Lobo


Ameneh Alizadeh,1 Amir Razmjou,1,2 Mehrorang Ghaedi,3 Ramin Jannesar4,5

1Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan 8174673441, Iran; 2UNESCO Centre for Membrane Science and Technology, School of Chemical Science and Engineering, University of New South Wales, Sydney 2052, NSW, Australia; 3Department of Chemistry, Yasouj University, Yasouj 75918-74831, Iran; 4Department of Pathology, Yasuj University of Medical Sciences, Yasuj 7591741417, Iran; 5Department of Biotechnology and Microbial Nanotechnology, Dena Pathobiology Laboratory, Yasuj 7591774414, Iran

Purpose: Nanoporous membranes have been employing more than before in applications such as biomedical due to nanometer hexagonal pores array. Biofouling is one of the important problems in these applications that used nanoporous membranes and are in close contact with microorganisms. Surface modification of the membrane is one way to prevent biofilm formation; therefore, the membrane made in this work is modified with carbon nanotubes.
Methods: In this work, nanoporous solid-state membrane (NSSM) was made by a two-step anodization method, and then modified with carbon nanotubes (NSSM-multi-wall carbon nanotubes [MWCNT]) by a simple chemical reaction. Techniques such as atomic force microscopy (AFM), energy dispersive X-ray (EDAX), field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR), contact angle (CA), surface free energy (SFE), protein adsorption, flow cytometry, and MTT assay were used for membrane characterization.
Results: The BSA protein adsorption capacity reduced from 992.54 to 97.24 (µg mL-1 cm-2) after modification. The findings of flow cytometry and MTT assay confirmed that the number of dead bacteria was higher on the NSSM-MWCNT surface than that of control. Adsorption models of Freundlich and Langmuir and kinetics models were studied to understand the governing mechanism by which bacteria migrate to the membrane surface.
Conclusion: The cell viability of absorbed bacteria on the NSSM-MWCNT was disrupted in direct physical contact with carbon nanotubes. Then, the dead bacteria were desorbed from the surface of the hydrophilic membrane. The results of this research showed that NSSM-MWCNT containing carbon nanotubes have significant antimicrobial and self-cleaning property that can be used in many biomedical devices without facing the eminent problem of biofouling.

Keywords: anodizing, alumina anodic membrane, antibacterial, anti-biofilm

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