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Mussel-Inspired Gold Nanoparticle and PLGA/L-Lysine-g-Graphene Oxide Composite Scaffolds for Bone Defect Repair

Authors Fu C , Jiang Y, Yang X, Wang Y, Ji W, Jia G

Received 12 July 2021

Accepted for publication 6 September 2021

Published 30 September 2021 Volume 2021:16 Pages 6693—6718

DOI https://doi.org/10.2147/IJN.S328390

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Dongwoo Khang

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Chuan Fu,1 Yikun Jiang,1 Xiaoyu Yang,1 Yu Wang,2 Wei Ji,3 Guoliang Jia1

1Department of Orthopaedic Surgery, The Second Hospital of Jilin University, Changchun, 130041, People’s Republic of China; 2Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, People’s Republic of China; 3School of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, People’s Republic of China

Correspondence: Guoliang Jia
Department of Orthopaedic Surgery, The Second Hospital of Jilin University, Changchun, 130041, People’s Republic of China
Tel +86 157 54306089
Email [email protected]

Purpose: Insufficient biological activity heavily restricts the application and development of biodegradable bone implants. Functional modification of bone implants is critical to improve osseointegration and bone regeneration.
Methods: In this study, L-lysine functionalized graphene oxide (Lys-g-GO) nanoparticles and polydopamine-assisted gold nanoparticle (AuNPs-PDA) coatings were applied to improve the biological function of PLGA scaffold materials. The effects of Lys-g-GO nanoparticles and AuNPs-PDA functionalized coatings on the physicochemical properties of PLGA scaffolds were detected with scanning electron microscopy (SEM), contact angle measurement, and mechanical testing instruments. In vitro, the effects of composite scaffolds on MC3T3-E1 cell proliferation, adhesion, and osteogenic differentiation were studied. Finally, a radial defect model was used to assess the effect of composite scaffolds on bone defect healing.
Results: The prepared [email protected]/Lys-g-GO composite scaffolds exhibited excellent mechanical strength, hydrophilicity and antibacterial properties. In vitro, this composite scaffold can significantly improve osteoblast adhesion, proliferation, osteogenic differentiation, calcium deposition, and other cell behaviour. In vivo, this composite scaffold can significantly promote the new bone formation and collagen deposition in the radial defect site and presented good biocompatibility.
Conclusion: The combination of bioactive nanoparticles and surface coatings shows considerable potential to enhance the osseointegration of bone implants.

Keywords: gold nanoparticles, graphene oxide, bone defect, poly(dopamine), PLGA, L-lysine

Graphical Abstract:

Introduction

With the development of tissue engineering technology, concern regarding degradable polymer scaffolds for bone defect treatment is increasing. Currently, a large number of natural materials and synthetic polymers have been prepared as bone implants and applied in the treatment of bone defects, such as gelatin, chitosan, polypeptide, polylactic acid and PLGA.1–5 Among many degradable polymer materials, PLGA is the most widely used because of its good biocompatibility, degradability and adjustable mechanical properties.6 Many studies have adopted PLGA to prepare bone tissue engineering scaffolds and applied them to treat bone defects.7,8 However, PLGA material has some disadvantages, such as hydrophobic surfaces, deficient osteogenic induction properties, and others.9,10 Therefore, many strategies have been employed to improve the bone repair ability of PLGA by function modification.11 A common modification is to blend some bioactive nanoparticles with PLGA to prepare composites, such as hydroxyapatite, graphene oxide, carbon nanotubes, and others.12–14 These nanoparticles can significantly improve scaffolds’ mechanical strength, regulate cell function, and facilitate bone cell growth and fusion of the material with the surrounding bone tissue. This strategy can effectively improve the bone repair capacity of PLGA, serving as a solution to overcome the defects of a single material.

In recent years, graphene oxide (GO) has been widely used in the functional modification of polymer materials due to its excellent physical and chemical properties. The particular surface structure and abundant functional groups provided GO with many excellent properties, such as good hydrophilicity, antibacterial properties, dispersibility, and mechanical strength.15–18 Moreover, many studies have demonstrated that GO have strong osteogenic induction ability.19,20 Some studies have discovered that GO can promote osteogenic differentiation by increasing cytoskeletal tension.21 Furthermore, the strong protein adsorption capacity of GO can attract the nutrients required for cell growth on the material surface, creating a better living environment.22 More importantly, the abundant oxygen-containing active groups such as carboxyl groups, hydroxyl groups, and epoxy groups, facilitate modification of the GO surface with bioactive factors to improve biological properties. Many studies have employed various active substances of amino acids, growth factors, drugs, and others, to further modify the surface of GO to improve the bone repair ability of composite materials.23–25 Although the addition of bioactive nanoparticles can effectively enhance the bone repair ability of polymer materials, this method still has some deficiencies, such as uneven surface distributions of nanoparticles. To maximize the bone repair ability of bone implants, many studies have used other modification methods combined with bio-active nanoparticles to enhance the biological properties of bone implants. For example, Gao et al uses both hydroxyapatite nanoparticles and gelatin coatings to improve the biological activity of PLGA materials, and the results show that the combination of these two modification methods can effectively enhance cell proliferation and osteogenic differentiation ability.6 Yang et al modified PLGA nanofibres by cellulose and polydopamine coating at the same time, and the results showed that the composite nanofibre surface had good applicability for cell adhesion, proliferation and growth.26 Furthermore, the introduction of cellulose can effectively accelerate the deposition rate of the polydopamine coating. These studies have demonstrated that different modification methods can simultaneously improve the function of scaffold materials to achieve complementary advantages, and this kind of multi-component composite has broad application prospects in the field of bone repair. In addition to blending with bioactive nanoparticles, surface modification is another classic strategy to improve PLGA material performance. Grafting or coating scaffold surfaces with bioactive substances, such as gelatin, metal nanoparticles, hydroxyapatite, and others, can effectively regulate cell functions effectively.27–29 Many studies have combined different material modification methods to maximize the bone repair ability of scaffold materials.

Gold nanoparticles (AuNPs) have attracted considerable attention in the field of bone repair due to their advantageous properties in biomedical applications such as good biocompatibility, osteogenic inducibility and easy functionalization.30–32 Recently, some studies have found that AuNPs could effectively promote the osteogenic differentiation and mineralization of cells, and ALP activity, and the expression of osteoblast-related genes (Runx-2, OPN, OCN) were significantly enhanced after cell contact with AuNPs.33–35 Other studies suggested that AuNPs could stimulate cells by regulating the (MPAK) -p38 and ERK/MAPK pathway, thus inducing cell osteogenesis.36,37 A recent clinical trial suggested that surface modification of titanium implants with AuNPs can promote bone interface formation and bone regeneration.38 In addition, AuNPs can effectively regulate the behavior of osteoblasts and also modify the osteoclast formation pathway. Therefore, these features render AuNPs very suitable for functional modification of bone repair materials. In the application process of gold nanoparticles, their concentration, properties, morphology and synthesis methods have a very important effect on their biological applications.39,40 However, the conventional blending method of AuNPs has some limitations with respect to enhancing the properties of materials. Effective blending requires AuNPs at high concentrations, which will induce biological toxicity and reduce the biological activity of bone repair materials. Therefore, many studies prefer to use coating technology to introduce AuNPs into bone repair materials, which can not only maintain effective contact between cells and AuNPs but also avoid toxicity generation to a large extent. At present, several methods are commonly been used to apply AuNPs coatings on the surfaces of materials, such as sputtering coating and physical/chemical vapour deposition; however, these methods generally require complex instrumentation. In recent years, the polydopamine reduction has become a popular method to prepare metal nanoparticle coatings. Dopamine can oxidize and autopolymerize in oxygen and alkaline environments, forming polydopamine (PDA) coatings on most solid substance surfaces.41 The adhesion and reducibility of polydopamine can reduce gold ions to AuNPs, and facilitate their adherence to the surface of a material. Furthermore, the PDA coating can increase the material surface wettability, and accelerate hydroxyapatite formation, which can further improve the bone repair ability of the material. Thus, polydopamine-assisted AuNPs functionalized coating combined with bioactive nanoparticles is a superior choice to improve bone implant performance and warrants further investigation.

In this study, we modified GO with L-lysine (Lys) by chemical grafting, and blended Lys-g-GO with PLGA to prepare composite porous scaffolds (PLGA/Lys-g-GO). Then, we applied AuNPs-PDA functionalized coatings to the scaffold surface with a polydopamine as the reducing agent. The physical and chemical properties of the scaffolds were characterized by SEM, contact angle measurement, mechanical testing and antibacterial experiments. We also studied the effects of [email protected]/Lys-g-GO composite scaffolds on cell proliferation, adhesion, osteogenic differentiation, and new bone regeneration. This research aims to improve the biological activity of PLGA through the above material modification methods and prepare composite materials with an excellent bone repair ability for bone defect repair.

Materials and Methods

A detailed schematic diagram of the scaffold preparation is shown in Figure 1.

Figure 1 A brief schematic drawing of the preparation of [email protected]/Lys-g-GO composite scaffolds.

Materials

PLGA (molecular weight=100,000, LA/GA=75/25) was obtained from ChangchunSinoBiomaterials Co., Ltd. GO was purchased from Chengdu Organic Chemicals Co. Ltd, China (thickness: 0.55–1.2nm diameter:0.5–3μm) (MFG Code 021519). L-lysine was purchased from Beijing Chemical Reagent Co., Ltd, China (Product number 62016734). HAuCl4·3H2O (CAS number 16961-25-4), Cetylpyridinium chloride (CPC) (CAS number 6004-24-6) and dopamine hydrochloride (CAS number 62-31-7) was purchased from Aladdin Reagents Co., Ltd, China. N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) (CAS number 25952-53-8), N-Methylpyrrolidone (NMP) (CAS number 872-50-4), 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) (CAS number 298-93-1), 4,6-diamino-2-phenylindole (DAPI) (CAS number 28718-90-3) and rhodamine-phalloidine (Product number P1951) were purchased from Sigma-Aldrich (USA). The reagents for cell experiments were purchased from Sigma-Aldrich (USA). Hematoxylin and eosin (H&E) (Product number G1120), Masson trichrome staining (Product number G1340) and Sirius Red kits (Product number S8060) were purchased from Solarbio Co., Ltd, China.

Preparation and Characterization of Lys-g-GO

L-lysine was grafted to the surface of GO by chemical grafting. In brief, 100 mg GO was first dispersed in 50 mL deionized water followed by continuous ultrasound for 2 h. Subsequently, 0.25 g EDC was added to the above solution to activate the carboxyl group of the GO. Then, 0.3 g L-lysine powder was added to the solution system, and stirred at room temperature for 24 h. After the reaction is complete, the mixed solution is centrifuged by a centrifuge (8000 rpm) (eppendorf, Centrifuge 5427 R, GER), and the resulting precipitate is rinsed repeatedly with deionized water. Finally, the Lys-g-GO were obtained with vacuum drying. Fourier transform infrared spectra (FTIR, Perkin Elmer, 1730, USA) were used to identify the crosslinking between L-lysine and GO. The surface structures of Lys-g-GO were observed by transmission electron microscopy (TEM, FEI, Tecnai G2 F20 S-TWIN, USA).

In order to compare the effects of GO and Lys-g-GO on the physicochemical properties of PLGA materials, we added GO and Lys-g-GO into PLGA respectively, and made the composite material into a film sheet (the ratio of PLGA to nanoparticles is 99: 1). Then, the surface hydrophilicity of composite samples was studied by water contact angle tester (Kruss, DSA 10, GER). The protein adsorption properties of PLGA, PLGA/GO and PLGA/Lys-g-GO were analysed by performing a bicinchoninic acid (BCA) protein assay (Abcam, ab207003) using BSA as a model protein. Briefly, the samples were added into 4 mL of BSA solution (2 mg/mL), and liquid samples were taken to analyse the solution BSA concentration after 1h. The amount of BSA loaded on the samples was determined by the reduction in BSA concentration.

Fabrication of PLGA/Lys-g-GO Composite Scaffolds

A preparation process combining phase inversion and particle leaching was used to prepare PLGA/Lys-g-GO composite scaffolds. Briefly, 2 g PLGA was dissolved in 10 mL NMP followed by the addition of 20 mg Lys-g-GO. After the above mixed solution is fully stirred, pore-foaming agent (Sodium chloride granule, 220~450 µm) were added to the solution, and the mixture is poured into cylindrical mould and frozen. After the mixed solution is completely frozen, the cylindrical mould was immersed in deionized water for 3 days, and the deionized water is changed every 8 hours. After the NMP solvents and sodium chloride granule in the mixed solution were completely replaced by water, the solidified scaffold materials were freeze-dried and stored in a drying oven.

PDA-Assisted Immobilization of AuNPs Onto PLGA/Lys-g-GO Composite Scaffolds

In this study, the surface of the scaffold material was functionally treated with polydopamine, and gold nanoparticles were reduced and loaded on the surface of the scaffold through the reducibility of polydopamine.42,43 The operation process was performed as followed. First, PLGA/Lys-g-GO composite scaffolds were immersed in dopamine solution (2 mg/mL, pH 8.5). After 24 h, the PDA modified scaffold ([email protected]/Lys-g-GO) was washed with deionized water to remove unattached dopamine molecules. Then, the scaffold was immersed in gold ion solution (2.0 mM) overnight at room temperature. The AuNPs coating was constructed on the surface of the scaffold by the reducibility of PDA. Finally, The [email protected]/Lys-g-GO scaffolds were then washed with distilled water for to remove the unattached AuNPs. At the same time, four groups scaffold materials were prepared as control group, including pure PLGA scaffolds, PLGA/Lys-g-GO scaffolds, PDA-modified PLGA scaffolds ([email protected]) and PDA-assisted AuNPs-coated PLGA scaffolds ([email protected]).

Composite Scaffolds Characterization

The composite scaffolds were cut off and the middle cross section was exposed. Then, a scanning electron microscope (SEM, Zeiss, EVO 25, GRE) was used to observe the microstructure of the composite scaffolds. The composite scaffold samples were prepared into cylinders with a diameter of 8 mm and a height of 25 mm, and the mechanical properties of the composite scaffolds was detected with a multifunctional mechanical tester (Instron, Instron 1121, UK). The hydrophilicity of samples was detected by water contact angle tester (Kruss DSA 10, GER). X-ray photoelectron spectroscopy (XPS, Kratos, JPN) and X-ray diffraction (XRD, Bruker. D8 ADVANCE, GER) were used to detect the elemental composition and crystalline structure of composite scaffolds.

Determination of Antibacterial Activity

Staphylococcus aureus (S. aureus, ATCC25923) and Escherichia coli (E. coli, ATCC25922) were used as strains to evaluate the antibacterial properties of composite scaffolds. All bacterial strains were provided by the Experimental Center of the Second Hospital of Jilin University. Briefly, composite scaffolds were first immersed in 75% ethanol solution and sterilized with UV. The sterilized scaffolds were then rinsed repeatedly with PBS to remove residual ethanol. The bacterial concentration was adjusted to 4×104 CFU/mL and cultured in a constant temperature incubator at 37°C for 2 h. Then, the bacterial was co-cultured with the sterilized composite scaffolds. After 12 h, 150 µL bacterial solution was transferred to a 96-well plate, and the absorbance value at 600 nm was measured by multifunctional microplate scanner (TECAN, Infinite M200, Swit). In order to further observe the antibacterial properties of composite scaffolds, 60 µL of bacterial liquid was absorbed and diluted. Then, 30 µL diluted bacterial solutions was evenly spread onto an LB agar plate and incubated at 37°C overnight. Finally, the colonies in the LB agar plate were photographed. Previous studies have proved that PLGA materials have no obvious antibacterial properties, so PLGA samples were selected as the control group.

Cell Adhesion and Proliferation Assay

Mouse pre-osteoblast MC3T3-E1 cells (Shanghai Institute of Cell Biology, China) were used as model cells to evaluate the bioactivity of the composite scaffold. Cells were cultured in high-glucose DMEM medium with 10% foetal bovine serum, 63 mg/L penicillin and 100 mg/L streptomycin at 37°C with 5% CO2, and the culture medium was refreshed every 2 days. For the cell proliferation examination, the composite scaffold was immersed in 75% ethanol solution and sterilized by UV. Then, the fully sterilized composite scaffold was rinsed repeatedly with PBS and soaked in cell medium. Finally, the cells were seeded at composite scaffold in the 24-well plate at a density of 2.5×104/ well. On days 1, 3, and 7, MTT assays were used to determine cell proliferation on different composite scaffold. At each time point, 80 µL MTT solution (5 g L−1 in PBS) was added to the culture well, and incubated for 4 h. After incubation, the medium was removed and 600 μL of acidified isopropanol was added to solubilize the converted dye. Finally, 150 μL dissolved liquid was transferred to a new 96-well plate, and the absorbance value at 540 nm was determined by multifunctional microplate scanner (TECAN, Infinite M200, Swit).

To evaluate cell adhesion, the adherent cells cultured on various films (PLGA, [email protected], [email protected], PLGA/Lys-g-GO and [email protected]/Lys-g-GO) were fixed with 4% paraformaldehyde after a 3-day culture. Subsequently, cells were washed by PBS to remove paraformaldehyde, and the cytoskeleton and nucleus were stained by phalloidin (red) and DIPI (blue), respectively. Finally, the adhesion cells on the surface of different materials were observed under fluorescence microscope (Nikon, E80i, Japan).

Cell Mineralization

Calcium deposition in cells growing on composite scaffold was detected by Alizarin red (ARS) staining. Briefly, the medium was removed, and cell was fixed with paraformaldehyde after 21 day culture. After the cells were fixed, the cells/scaffold samples were rinsed with PBS, and 40mM ARS (Solarbio, G8550) was added and incubated for 30 min at room temperature. Then, the cells/scaffold samples were washed with distilled water for 3 times, ARS-stained samples were treated with 10% (w/v) CPC at room temperature for 15 min. Finally, 150 µL solutions was moved to 96 wells, and the absorbance value was measured at 540 nm for the calcium quantification analysis. In order to further intuitively observe the effect of composite scaffolds on cell mineralization, PLGA, [email protected], [email protected]@PLGA, PLGA/Lys-g-GO and [email protected]/Lys-g-GO films were prepared and treated under similar conditions to the composite scaffolds. After cells grew on films surface for 21 days, cells were stained with ARS and observed under a microscope (Nikon, E80i, Japan).

Quantitative Real-Time PCR Analysis

After cells were cultured on different composite scaffolds for 7 days, the osteogenic related gene expression of cells was detected by real-time quantitative PCR (RT-PCR). The total RNA was extracted using TRIzol Reagent (Invitrogen, USA) according to the manufacturer’s protocol. Then, the cDNA was synthesized using a PrimeScriptTM RT reagent kit (Takara Bio, Japan) according to the manufacturer’s instructions. RT-PCR was performed by Mx3005 (Stratagene, USA) and osteogenesis-related genes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), type 1 collagen (COL-I), runt-related transcription factor 2 (Runx2) and osteopontin (OPN) were assessed. The primer sequences are shown in Table 1. All experiments were done in triplicate to obtain the average data.

Table 1 List of Genes and Primer Nucleotide Sequences

Immunofluorescence Staining

In order to further detect the effect of different composite scaffolds on cell osteogenic differentiation, films were prepared and treated under similar conditions to the composite scaffolds, and cells were seeded on the surface of films. After 7 days of cell culture, the cells were fixed with paraformaldehyde and rinsed with PBS. Then, the cells were perforated with 0.2% (v/v) Triton X-100 (CAS number 9002-93-1) and then incubated with 1% BSA solution. COL-I (Abcam, ab34710), Runx2 (Abcam, ab114133) and OPN (Abcam, ab63856) primary antibodies were added into the cells and incubated overnight under 4°C overnight. After the primary antibody was incubated, FITC-labeled secondary antibody was added into cells and incubated at room temperature for 60 min. The cells were then stained using DAPI for 1 min, and the cells were observed under a fluorescence microscope (Nikon, E80i, Japan).

Repair for Rabbit Radius Defects

Male New Zealand white rabbits (2.5 kg) randomly fell into five groups (PLGA, [email protected], [email protected], PLGA/Lys-g-GO and [email protected]/Lys-g-GO, four animals per group) and were used for the radial defect implantation of scaffolds. All experimental animals were provided by Liaoning Changsheng Biotechnology Company, and kept in the Animal Room of Public Health College of Jilin University. In brief, after the experimental animals were completely anesthetized, the forelimb hair of rabbit was removed and the skin was disinfected with iodine. Then, a 3 cm incision was made in the middle radius on experimental animal, and the radius of about 20 mm was removed with a chainsaw. Then, the scaffold material was trimmed and the defect site was implanted. Finally, the epidermal wound was sutured and treated with regular disinfection and bandaging. Within 7 days after surgery, penicillin was injected intramuscularly to all experimental animals at a daily dose of 400,000 units, and observed the animals’ defecation, wound infection and other conditions every day.

X-Ray and CT Examination

On postoperative weeks 0, 4 and 12, the effect of different composite scaffolds on bone regeneration was examined with Digital Radio graph (Kodak, CR-400 plus, USA). According to the X-ray results, the new bone regeneration was evaluated by Lane-SandhuX ray score (Table 2). At the same time, 12 weeks after the experiment, the experimental animals were sacrificed and the radial tissue was removed. After the periosseous soft tissue was removed, the radius tissue was fixed with paraformaldehyde and the bone tissue was reconstructed by CT scanner (General Electric, ImatronC300, USA) in 3 D.

Table 2 Lane-Sandhu Radiographic Scoring System

Histological Evaluation

On postoperative weeks 12, bone tissue samples were taken out and fixed with paraformaldehyde. Then, bone samples were completely decalcified in 15% EDTA solution. After decalcification, samples were embedded in paraffin, and cut into tissue slices. Subsequently, tissue slices were stained with Masson trichrome, H&E and Sirius Red in accordance with standard protocols, and observed using light microscope (Nikon, E80i, Japan) and polarization microscope (Nikon, Eclipse Ci-L, Japan). The ratio of type I and type III collagen was quantitatively analyzed by Image-pro Plus 6.0 software (Media Cybernetics, USA).

Bio-Safety Assessment

On postoperative weeks 12, the experimental animals were sacrificed and a healthy New Zealand white rabbit without surgical treatment was taken as the control group. Important internal organs including heart, liver, spleen and kidney were removed from all experimental animals. Then, a series of slices were cut from the organs tissue and stained with H&E. Finally, the physiological structure of important organs in the experimental animals was observed under a microscope (Nikon, E80i, Japan) to evaluate the biological safety of the composite scaffold.

Statistical Analysis

All quantitative date were analyzed by one-way analysis of variance using Origin 8.0 software (Origin Lab Corporation, USA). Data are expressed as the mean ± SD. P value less than 0.05 was considered statistically significant in all analyses.

Results and Discussion

Characterization of the GO and Lys-g-GO

In this study, the GO surface was modified with L-lysine by chemical grafting. We first studied the chemical composition of Lys-g-GO nanoparticles by Fourier infrared spectroscopy (FITR). As shown in Figure 2A, the FITR spectrum of GO exhibited three characteristic peaks at 1052 cm−1, 3417 cm−1, and 1730 cm−1, which represented the C-O bond, the “O-H stretching” band of hydroxyl groups, and the “C=O stretching” band of carboxylic acids (black arrow), respectively. The results indicated the presence of abundant oxygen-containing groups on the GO surface. After grafting L-lysine onto the GO surface, the C=O bond representing the amide and carboxylic acid groups changed significantly in the range of 1620 ~ 1630 cm−1 (red arrow). In addition, the stretching peaks representing OH and NH also changed significantly at 3420 cm−1 (blue arrow). The results suggested successful grafting of L-lysine onto the GO surface. The TEM observation of the surface morphology of GO and Lys-g-GO revealed that GO presented flake like and wrinkled forms, which are similar to peeled wrinkled flakes, as shown in Figure 2B. Adhered with many substances resembling crystal structure, the surface of Lys-g-GO was rougher than the surface of GO. We speculated that the surface attached amino acids might lead to the formation of this morphology. The above FITR and TEM results proved that L-lysine was successfully grafted onto the GO surface.

Figure 2 (A) FITR spectra and (B) TEM images of GO and Lys-g-GO nanoparticles, All scale bar are 1μm. (C) Contact angle analysis of PLGA, PLGA/GO and PLGA/Lys-g-GO composite. (D) BSA adsorption efficiency of PLGA, PLGA/GO and PLGA/Lys-g-GO composite, *P < 0.05, n=3.

Influence of GO and Lys-g-GO on PLGA Materials

First, we prepared PLGA/Lys-g-GO and PLGA/GO films through blending and studied the effects of Lys-g-GO and GO on the physicochemical properties of PLGA. As shown in Figure 2C, the water contact angle of PLGA is 95.5±2.61°, the water contact angle of the composites significantly decreased to 85.8±3.17° after GO addition. Abundant oxygen-containing functional groups such as hydroxyl, carboxyl and epoxy groups, are present on the surface of GO, which confer GO good hydrophilicity. Compared with PLGA/GO, the surface hydrophilicity of PLGA/Lys-g-GO composites further improved as the contact angle decreased to 79.8±5.5°. Lysine with strong hydrophilicity can provide hydrophilic groups such as amino groups and carboxyl groups for materials. Grafting lysine onto the GO surface can increase the number of hydrophilic groups of materials to some extent. Figure 2D shows the protein adsorption capacity of each group within one hour. The protein adsorption capacity test with BSA as the model protein demonstrated that the addition of Lys-g-GO to PLGA enhanced the protein adsorption capacity of the composite material, which was significantly higher than that in the PLGA/GO group (P<0.05). We speculated that lysine increases the positive charge of the GO surface in the physiological environment, enhancing the electrostatic interaction between the material and negatively charged BSA. The excellent protein absorption of the material could quickly generate a layer of protein film on the surface to supply more binding sites, improving cell adhesion and migration.

To further study the effect of GO and Lys-g-GO on the biological activity of PLGA materials, we planted MC3T3-E1 cells on different material surfaces and detected the proliferation, adhesion, and osteogenic differentiation of the cells by MTT assay, FITC staining, and PNPP assay, respectively. The results showed that compared with GO, Lys-g-GO had no significant effect on cell proliferation but significantly promote cell adhesion. Analysis of cell images (Figure S1) indicated that the cell growth status and area percentage in the PLGA/Lys-g-GO group were better than those in the PLGA/GO group (P < 0.05) after 3 days of growth. Moreover, among the two nanoparticles, Lys-g-GO more obviously improved the osteogenic induction ability of PLGA. The ALP level in the PLGA/Lys-g-GO group was significantly higher than that in the PLGA group, especially on the seventh day (P < 0.05) (Figure S2). Many studies found that lysine could regulate the expression of integrin genes in cells, promoting extracellular matrix formation.44 Simultaneously, hydroxylysine is the main component of collagen, which can promote the synthesis and mineralization of collagen in the extracellular matrix. Therefore, compared with unmodified GO, Lys-g-GO can further improve the hydrophilicity, protein absorption, and osteogenic induction ability of PLGA.

Characterization of Composite Scaffolds

Considering the excellent biological activity of Lys-g-GO, we prepared PLGA/Lys-g-GO composite scaffolds by the particle leaching/phase transformation method using PLGA and Lys-g-GO as matrix materials. Additionally, we applied AuNPs-PDA functionalized coatings to the surfaces of the scaffolds by reducing gold ions in the solution to AuNPs with polydopamine. As shown in Figure 3, all scaffolds present very typical through-hole structures with channels interconnecting and running through the whole scaffold. In addition, microchannel structures exist on the macropore surfaces of the scaffolds, the size of which ranges from nanometres to more than ten microns, which can effectively improve nutrient exchange inside the scaffold and show advantages in regulating cell function over the flat pore surface. The pure PLGA scaffold is pure white. After Lys-g-GO addition, the general appearance of the scaffold showed no significant change, but the colour was slightly deepened to light grey. After surface modification with polydopamine, the scaffold was further darkened to black, and the whole scaffold appeared dark brown after loading AuNPs. The pore surface of PLGA is relatively flat with scattered microchannel structures. The addition of Lys-g-GO significantly increased the size and number of microchannels on the surface, which may be related to the layered PLGA material caused by the addition of nanoparticles, thus forming more microchannel structures. [email protected] and single PLGA scaffolds had a similar morphology and structure, but the surface of the scaffolds was rougher, and the microchannel diameter reduced, probably due to the polydopamine coating. After loading AuNPs, some agglomerated gold nanoparticles were found on the surface of the scaffold.

Figure 3 (A) Macroscopic view and (B and C) SEM images of PLGA (a), [email protected] (b), [email protected] (c), PLGA/Lys-g-GO (d) and [email protected]/Lys-g-GO (e) scaffolds. Bar lengths are 400μm (B) and 40 μm (C).

We further analysed the surface chemical composition of different scaffolds to confirm functional modification. Figure 4A and B shows the XPS high-resolution scanning spectrum of the scaffold surface with evident peak intensity changes for elements on different scaffold surfaces. The prominent peaks on the surface of the PLGA scaffolds were C1S and O1S. After surface modification of PLGA scaffolds with polydopamine, a prominent N1S peak appeared at 400 eV, which was caused by the introduction of a surface amine. The elemental composition of the PLGA/Lys-g-GO scaffolds was the same as that of the PLGA scaffolds, and no obvious N1S peak, which may be related to the low Lys-g-GO content. The peak of Au1S was detected after the scaffolds were loaded with AuNPs. The high-resolution spectrum of Au confirmed the presence of zero-valent gold, indicating that AuNPs had deposited on the surface of the scaffolds. We detected the crystal structure of different scaffold samples through XRD. Figure 4C shows the XRD patterns of different scaffold samples. We found peaks in the range 13–27 at the 2θ position of different samples, which was the characteristic peak of PLGA. After modifying scaffolds with polydopamine, the XRD pattern did not change significantly, indicating that polydopamine had no obvious effect on the crystal structure of the scaffolds. After adding Lys-g-GO to the scaffolds, the XRD patterns of the PLGA/Lys-g-GO samples showed a characteristic peak with a corresponding layer spacing of approximately 0.79 nm at the position of 11 (black arrow), which was a typical characteristic peak of GO, demonstrating the existence of Lys-g-GO in the scaffolds. After AuNPs were loaded on the scaffolds, the characteristic peak of gold appeared at 38, 44, 65, and 78 (yellow arrow). The XPS and XRD results confirmed the completion of scaffold surface modification.

Figure 4 (A) XPS, (C) XRD patterns and (B) the detailed diagram of the Au 4f counts for PLGA (a), [email protected] (b), [email protected] (c), PLGA/Lys-g-GO (d) and [email protected]/Lys-g-GO (e) scaffolds.

Contact Angle Analysis

Surface hydrophilicity can significantly affect bone implant material biocompatibility. A hydrophilic surface can effectively promote cell infiltration and adhesion. In this research, we evaluated the wettability of scaffold materials by measuring water droplets contact angles on the surfaces of different materials. As shown in Figure 5A and B, the PLGA contact angle decreased significantly after incorporation of Lys-g-GO nanoparticles. After surface modification with polydopamine, the contact angle of the scaffold was further significantly reduced to 29.1±6.27°, probably due to the rich hydrophilic catechol and nitrogen-containing groups in polydopamine. After the scaffolds were loaded with AuNPs, we found that the contact angle of the scaffold surface increased to a particular level (43.3±5.06°), which might be due to the hydrophobic AuNPs affecting the binding of the surface elements of the scaffolds with water to some extent. Studies have suggested that the cell adhesion ability significantly improves when the material contact angle is between 0° and 40°.45 The [email protected]/Lys-g-GO scaffold surface contact angle was 38.7±7.85°, which might create a more suitable environment for cell growth.

Figure 5 (A and B) Contact angle analysis, (C) Compression strength and (D) Elastic modulus of PLGA (a), [email protected] (b), [email protected] (c), PLGA/Lys-g-GO (d) and [email protected]/Lys-g-GO (e) scaffolds. *P<0.05, error bars represent standard deviation for n=3.

Mechanical Properties of Composite Scaffolds

Scaffolds can not only fill the bone defect site but also provide mechanical support when treating bone defects. Therefore, the mechanical strength of scaffolds is very important for bone repair, especially when applied to weight-bearing sites. We analyzed the compressive strength and elastic modulus of different scaffold materials by mechanical testers. As shown in Figure 5C and D, the compressive strength and elastic modulus of the pure PLGA scaffolds were 1.68±0.22MPa and 3.06±1.02MPa, respectively. After the addition of Lys-g-GO, the compressive strength and elastic modulus of the scaffolds increased to 2.79±0.15MPa and 7.44±2.94Mpa, respectively. The results indicated that Lys-g-GO nanoparticles could effectively improve scaffold mechanical properties. GO can interact with polymers through π-π interactions, van der Waals forces, and hydrogen bonding, stabilizing the material structure, improving the interface interaction, and effectively enhancing the mechanical strength of materials.46 The compressive strength of the [email protected], [email protected] and [email protected]/Lys-g-GO scaffolds were 1.93±0.22, 1.8±0.16, and 2.68±0.21 MPa, respectively. The elastic modulus of the [email protected], [email protected] and [email protected]/Lys-g-GO scaffolds were 3.1±1.49, 3.9±2.25 and 7.2±1.47MPa, respectively. The compressive strength and elastic modulus of the scaffolds modified with polydopamine or loaded with AuNPs did not change significantly (P > 0.05). The results showed that the AuNPs-PDA functionalized coating did not have a negative effect on the intrinsic mechanical strength of the scaffolds, and the compressive strength of the scaffolds was similar to that of human cancellous bone, which can be an ideal bone replacement material.

Antibacterial Activity of Composite Scaffolds

An ideal bone implant material should also have certain antibacterial ability, such that it can reduce the use of antibiotics, prevent bacterial infection and improve the success rate of surgery. Recently, many studies have found that GO and AuNPs can improve the antibacterial properties of implant materials to a certain extent.47,48 Therefore, in this study, S. aureus and E. coli were used as experimental strains to detect the effects of AuNPs-PDA functionalized coatings and Lys-g-GO nanoparticles on the antibacterial properties of PLGA materials. In our previous study, PLGA material was demonstrated to have no obvious antibacterial activity, thus, PLGA was used as the experimental control group.49 As shown in Figure 6A, the [email protected] group showed a similar OD value as the PLGA group, indicating that the PDA coating had no obvious effect on the antibacterial properties of the material. After loading AuNPs on the surface of the PLGA scaffolds, the OD value of bacterial solutions decreased to a certain extent, but the decreasing trend was not obvious, and a significant difference was found only when scaffold was co-culture with E coli (P < 0.05). Among all scaffolds, the PLGA/Lys-g-GO and [email protected]/Lys-g-GO groups showed the highest bacterial inhibition, and a significant difference was observed compared with the PLGA group (P < 0.05), indicating that Lys-g-GO can enhance the antibacterial activity of PLGA materials. To more intuitively observe the antibacterial properties of different scaffold materials, bacteria were separated from the scaffold sample and plated on LB agar plates, and the antibacterial effects of scaffold materials were observed based on the formation of bacterial colonies. As shown in Figure 6B, a large number of colonies were found in the PLGA and [email protected] groups, which almost covered the whole LB culture plate. The [email protected], PLGA/Lys-g-GO and [email protected]/Lys-g-GO groups performed much better than the PLGA and [email protected] groups in reducing bacterial numbers. Bacterial inhibition was the most obvious in PLGA/Lys-g-GO and [email protected]/Lys-g-GO groups.

Figure 6 (A) Comparison of the antibacterial efficiency of different composite scaffolds, and (B) Photographs of bacterial colonies formed by E. coli and S. aureus treated with different composite scaffolds. PLGA (a), [email protected] (b), [email protected] (c), PLGA/Lys-g-GO (d) and [email protected]/Lys-g-GO (e). *P<0.05, error bars represent standard deviation for n=3.

According to the above experimental results, both the AuNPs-PDA functionalized coating and Lys-g-GO can improve the antibacterial activity of PLGA scaffolds to varying degrees. However, compared with Lys-g-GO, AuNPs-PDA coatings have less antimicrobial effects. Some studies have found that free AuNPs can enter the bacterial interior through the cell membrane and destroy the internal structure of the bacteria by inducing oxidative stress.50 However, the antimicrobial activity of AuNPs was directly related to the concentration of AuNPs. Some studies have found that AuNPs concentrations above 3.125 and 6.25μg/mL had inhibitory effects on bacteria.51 Therefore, we speculated that the content of AuNPs loaded on the PLGA scaffold surface by PDA coating was low and could not achieve an effective antibacterial concentration. Compared that of the AuNPs-PDA functionalized coating, the antibacterial performance of the scaffold material was improved more obviously by Lys-g-GO. The antibacterial mechanisms of GO materials are mainly divided into two pathways: mechanical damage and induced oxidative stress. First, GO can use its sharp edge structure to cut through the bacterial cell wall, and this mechanical action will destroy the structural integrity of bacteria.52 However, this mechanical action mainly occurs in the GO suspension state. When GO is uniformly distributed in a polymer material, this mechanical damage effect is markedly reduced. Another reason for the excellent antibacterial properties of GO is that GO can induce oxidative stress reactions in bacteria, thus increasing the pressure on the bacterial cell membrane and destroying the bacterial structure.53 We speculated that this characteristic in GO was the main reason for the improved antibacterial properties of the scaffold materials. Furthermore, lysine molecules can increase the number of positive charges of GO, thus, the location of Lys-g-GO on the surface of the scaffold easily captures negatively charged bacteria, and further enhances the antibacterial properties of scaffold materials. More importantly we found that the AuNPs-PDA coating and Lys-g-GO had a synergistic effect on improving the antibacterial properties of scaffold materials. Overall, the [email protected]/Lys-g-GO composite scaffold has good antibacterial properties, which can prevent the occurrence of infection and markedly increase the success rate of implantation surgery.

Cell Proliferation and Adhesion

Changes in the surface properties of scaffolds can interact with cells, affecting cell adhesion, proliferation, differentiation, and other behaviours. The combination of AuNPs-PDA coating and Lys-g-GO could significantly change the surface morphology of PLGA scaffolds and improve their hydrophilicity, mechanical strength, and antibacterial properties. We planted MC3T3-E1 cells on the surfaces of different scaffolds and detected cell proliferation to confirm whether these changes in material properties have a positive effect on bone regeneration. Figure 7A shows the proliferation of cells on different scaffold surfaces. After 1 day of culture on different scaffolds, no significant change in cell proliferation was found among each group. On the 3rd day, the proliferation rate of cells in the [email protected] group was significantly higher than those in PLGA and PLGA/Lys-g-GO group (P < 0.05), demonstrating that polydopamine modification can effectively improve the cell proliferation. However, a slight decrease in the cell proliferation rate was noted after loading AuNPs on the scaffold surfaces (P > 0.05). After 7 days of cell culture, cell proliferation in each group was similar to that on the 3rd day, but cell proliferation in the [email protected]/Lys-g-GO group improved to a particular extent, and the cell proliferation rate was similar to that in the [email protected] group, and significantly higher than that in PLGA/Lys-g-GO group (P < 0.05).

Figure 7 (A) Cell proliferation and (B) morphology of MC3T3-E1 cells cultured on PLGA (a), [email protected] (b), [email protected] (c), PLGA/Lys-g-GO (d) and [email protected]/Lys-g-GO (e) scaffolds. Scale bar lengths are 200 μm, *P<0.05, error bars represent standard deviation for n=3.

We created a membrane sheet of scaffold materials in each group and planted MC3T3-E1 cells to further observe the effect of scaffold materials on cell adhesion. After 3 days of cell culture, we observed the adhesion and spread of cells on the material surface. As shown in Figure 7B, although the cells grew well on the surface of pure PLGA materials, the number of cells was significantly lower under a single field of vision, and most of the cells were not fully spread out. After modifying the material surface with polydopamine, the adhered cells exhibited greater numbers, a larger adhered area, and a better spreading state than those in the PLGA and PLGA/Lys-g-GO groups. In the [email protected], [email protected] and [email protected]/Lys-g-GO groups, the cells were almost completely covered material and stacked on top of each other, and the interaction between the cells was more prominent, indicating the strong adhesion behaviour of the cells.

The above results suggested that polydopamine could improve the cell adhesion and proliferation, which may be related to improved hydrophilicity and the abundant catechol-based adhesion groups in polydopamine.54,55 However, we found a slight decrease in cell proliferation after loading AuNPs on the scaffold surfaces. Loading of AuNPs, a hydrophobic metal substance, could reduce the material surface hydrophilicity and the exposure of the catechol-based adhesive groups on the scaffold surface to some extent, which may affect the early adhesion and proliferation of the cells in a particular range. In addition, we found the cell proliferation in the [email protected]/Lys-g-GO group was slightly lower in the early stage than that in the [email protected] group, but was significantly improved in the later stage of proliferation. According to the experimental results, Lys-g-GO could also effectively enhance cell proliferation and adhesion behaviour, which might have a particular synergistic effect with the polydopamine coating, overcoming the adverse effects of AuNPs to some extent. The experimental results of cell proliferation and adhesion indicated that the PLGA/Lys-g-GO scaffolds modified by the AuNPs-PDA functionalized coating can significantly enhance cell adhesion and proliferation.

Cell Mineralization

Cellular mineralization is a marker of mature bone differentiation, and is widely used to confirm the degree of osteocyte differentiation and the secretion of mineralized extracellular matrix. We evaluated the effects of different scaffold materials on MC3T3-E1 cell mineralization by ARS staining and calcium quantification. As shown in Figure 8A, the PLGA group exhibited the lowest OD value in all groups, demonstrating that the cell calcium deposition in the PLGA scaffolds was the lowest. After modification with polydopamine, the OD value of the [email protected] group was significantly higher than that of the PLGA group (P<0.05), demonstrating that the calcium deposition content of cells effectively increased. After loading AuNPs on the scaffold surface, the calcium deposition content of cells further increased, and the OD value was close to that in the PLGA/Lys-g-GO group, which was significantly higher than that in the [email protected] group (P<0.05). Due to the limitation of scaffolds’ three-dimensional morphology, we could not directly observe the cell mineralization. Thus, we fabricated different scaffold materials into film sheets and implanted cells. After 21 days of culture, ARS staining was performed for observation. As shown in Figure 8B, the PLGA group had lighter staining and fewer calcium nodules, while the staining intensity and red calcium nodule density in the [email protected] group significantly increased, indicating that polydopamine could promote calcium deposition in the extracellular matrix. Polydopamine can adsorb Ca and P ions on the surface of the material through its adhesion ability, thus accelerating the rate of cell mineralization.56 After loading AuNPs, the number of mineralized nodules increased further. Among the sample groups, the [email protected]/Lys-g-GO group exhibited the largest staining area, and red mineralized nodules almost covered the whole field of vision, demonstrating that the AuNPs-PDA functionalized coating and Lys-g-GO presented a synergistic effect in improving cellular calcium deposition.

Figure 8 (A) The corresponding quantitative evaluation of calcium content mineral deposition in different composite scaffolds. (B) Alizarin Red staining of MC3T3-E1 cells cultured on different films at 21d. PLGA (a), [email protected] (b), [email protected] (c), PLGA/Lys-g-GO (d) and [email protected]/Lys-g-GO (e). Scale bar lengths are 200 μm, P<0.05, error bars represent standard deviation for n=3.

Bone-Related Gene and Protein Expression

Bone regeneration involves processes such as cell adhesion, proliferation, osteogenic differentiation and calcium deposition, which are regulated by various genes. For example, the osteogenic transcription factor (Runx-2) is a typical gene expressed early in osteogenic differentiation.57 Osteopontin (OPN) is a crucial component of the bone extracellular matrix and can be greatly expressed in the middle and late stages of cell osteogenic differentiation.27,58 Type I collagen (COL-I) is expressed at the early stage of extracellular matrix secretion, and its expression level gradually increases with cell differentiation and maturation.59 Therefore, in this study, the Runx-2, OPN and COL-I genes were selected to detect the bone-related gene expression of cells by RT-PCR. As shown in Figure 9A, after 7 days of cell culture with different scaffolds, the expression level of COL-I in each group increased to some extent compared with the PLGA group. COL-I expression in [email protected]/Lys-g-GO group was significantly higher than that in the PLGA group (P < 0.05). Runx-2 expression was not significantly different between the PLGA and [email protected] groups, indicating that polydopamine had no significant effect on inducing early cell osteogenic differentiation. The expression level of Runx-2 in the [email protected] group significantly increased (P <0.05) compared with that in the [email protected] and PLGA groups. The Runx-2 level of the cells was further enhanced after modification with AuNPs-PDA functionalized coating and Lys-g-GO. Comparing the PLGA/Lys-g-GO group, we found that the Runx-2 level in the [email protected] group was higher, indicating that AuNPs were slightly superior to Lys-g-GO in regulating early osteoblast differentiation. Regarding the expression of OPN, we found that the expression of OPN in the [email protected] group was significantly higher than that in the PLGA group (P< 0.05). After modifying the scaffolds with AuNPs-PDA coating and Lys-g-GO, OPN expression in cells further increased, and the expression level was significantly higher than those in the [email protected] and PLGA group (P < 0.05).

Figure 9 (A) Quantitative real-time PCR analysis and (B) immunofluorescent images for COL-I, Runx-2 and OPN expression by MC3T3-E1 cells seeded on PLGA (a), [email protected] (b), [email protected] (c), PLGA/Lys-g-GO (d) and [email protected]/Lys-g-GO (e) composite scaffolds. DAPI staining for nuclei (blue) and FITC-conjugated secondary antibody for protein (green). *P<0.05, error bars represent standard deviation for n=3, scale bar lengths are 200 μm.

We detected the protein expression of osteogenic factors (COL-I, OPN, and Runx2) by protein immunofluorescence staining to further examine the osteogenic differentiation ability of cells on different scaffold surfaces. As shown in Figure 9B, the PLGA group had the lowest COL-I expression, while a high level of COL-I protein expression appeared in the other groups. After loading with AuNPs, greater Runx2 expression was observed in the fluorescence images of the samples, which was significantly higher than that in the other groups. In terms of OPN protein expression, we found that protein expression in the [email protected] group was higher than that in the PLGA group. After loading AuNPs or the addition of Lys-g-GO, OPN expression was further improved in the samples. In particular, a large area of OPN protein expression appeared in the [email protected]/Lys-g-GO group. The expression analysis of proteins and genes related to osteogenic differentiation showed a high degree of agreement between the two, demonstrating that [email protected]/Lys-g-GO scaffolds could effectively promote cell osteogenic differentiation.

The above experimental results suggested that both the AuNPs-PDA coating and Lys-g-GO could effectively improve the osteogenic induction ability of scaffolds and promote the expression of genes and proteins related to osteogenic differentiation. Many studies demonstrated the strong osteogenic induction ability of GO, and the co-culture of GO and cells could effectively improve the osteogenic differentiation ability of cells, which has been proven again in our research.60,61 AuNPs could induce a cellular stress response by entering cells, activating the p38 MAPK signalling pathway to induce the upregulation of genes related to osteoblast differentiation.62 In addition, polydopamine improved scaffold hydrophilicity and adhesion, promoted the deposition of calcium and phosphorus ions, and enhanced the osteogenic induction of scaffolds.

In vivo Assessment of Bone Regeneration

We established rabbit radius defect models and repaired the defect models with different scaffolds to further evaluate the bone repair ability of different scaffold materials. As shown in Figure 10A, we created a 20mm defect in the radius of the rabbit and repaired the defect with different scaffold materials. We first observed the healing of the radius by X-ray in different experimental animals. As shown in Figure 10B, neat and clear defects of approximately the same length were found in the radius of all experimental animals at 0 weeks postoperatively; less new bone formation were observed in the PLGA group at 4 weeks, and defect connection and repair were not completed in the following 12 weeks. The results demonstrated the limited bone repair ability of the PLGA scaffold. At 4 weeks, more new bone tissue appeared in the defect sites of the PLGA/Lys-g-GO group and [email protected]/Lys-g-GO group, and the defect sites had achieved preliminary connections compared with the PLGA group. Although large amounts of new bone tissue were also found in the defect site in the [email protected] group, the amount of bone tissue was slightly less than those in the PLGA/Lys-g-GO group and [email protected]/Lys-g-GO group. At 12 weeks, the defect sites of the [email protected] group, PLGA/Lys-g-GO group, and [email protected]/Lys-g-GO group were filled with new bone with the convergence of bone edges and the morphology close to normal bone tissue. The defects were not completely filled in the [email protected] group, but the amount of new bone was slightly higher than that in the PLGA group. Then, we conducted Lane-Sandhu X-ray scoring on the bone regeneration of different groups. Figure 10D shows that the [email protected]/Lys-g-GO group had the highest repair effect score, while the PLGA group had the lowest repair effect score. The repair effect score was as follows: [email protected]/Lys-g-GO (7.25±2.21)>PLGA/Lys-g-GO (6.75±2)>[email protected] (6±1.41)>[email protected] (3.25±1.25)>PLGA (2.75±1.5). We took the radius samples from experimental animals 12 weeks postoperatively and conducted three-dimensional reconstruction by a CT-scanner, as seen in Figure 10C. The repair effect of the [email protected]/Lys-g-GO, PLGA/Lys-g-GO, and [email protected] groups was more significant, and the radius shape was close to that of normal bone tissue. The bone defect was visible in the radial centre of the PLGA group and [email protected] group.

Figure 10 (A) Diagram of animal experiment process, (B) X-ray, (C) CT reconstruction image and (D) Lane−Sandhu radiographic scores, PLGA (a), [email protected] (b), [email protected] (c), PLGA/Lys-g-GO (d) and [email protected]/Lys-g-GO (e) scaffold treatment groups. *P<0.05, error bars represent standard deviation for n=4.

Histologic Analysis

We analysed the bone formation and collagen deposition at the defect site by HE, Masson, and Sirius red staining. As shown in Figure 11A, the scaffold in each experimental group were decomposed into fragments and scattered among tissues. After 12 weeks, the new bone formation was poor in PLGA group and [email protected] group. The tissue of the defect site was mainly hyperplastic connective tissue, and only a small amount of bone tissue was observed. In contrast, the repair effects of the [email protected]/Lys-g-GO group, PLGA/Lys-g-GO group, and [email protected] group were significantly better than those of other two groups. The amount of new bone at the bone defects was larger with many formations of lamellar bone, suggesting the excellent repair effect of the implant. Figure 11B shows the Masson staining results, a small number of blue collagen fibre was found in the PLGA group. In contrast, we found more blue collagen fibre in the [email protected] group, indicating that surface modification with polydopamine could promote collagen secretion in the bone defect sites. More mature collagen fibre appeared in the PLGA/Ly-g-GO and [email protected] groups, accompanied by an osteoid matrix. Moreover, we found that the [email protected]/Lys-g-GO group contained the largest collagen fibre area, and blue collagen fibres covered most of the defective tissues.

Figure 11 Histological analysis using H&E (A), Masson trichrome (B), Sirius red (C) staining and the COL-I/COL-III area ratio (D). PLGA (a), [email protected] (b), [email protected] (c), PLGA/Lys-g-GO (d) and [email protected]/Lys-g-GO (e). Magnification, 200×.

In the organic components of normal bone tissue, the COL-I content is more than 90%. COL-I can provide binding sites for bone tissue biomineralization and promote cell differentiation, determining the performance of new bone to a large extent. Therefore, COL-I can evaluate the maturation of new bone to a certain extent. This research employed Sirius red staining to evaluate the collagen composition in sample tissues. As shown in Figure 11C, the amount of COL-I in all groups was higher than that of COL-III, indicating that the defect had passed the initial stage of fracture repair. However, the red/yellow region was larger in the [email protected]/Lys-g-GO group and PLGA/Lys-g-GO group, and the red/yellow region was smaller while the green region was larger in PLGA group and [email protected] group. As shown in Figure 11D, the COL-I/COL-III ratio of the PLGA, [email protected], [email protected], PLGA/Lys-g-GO and [email protected]/Lys-g-GO group were approximately 4.5±1.54, 3.95±1.11, 5.27±1.33, 6.74±1.73 and 9.53±1.23, respectively. Analysis of the proportion of COL-I and COL-III demonstrated a significantly higher proportion of COL-I/COL-III in the [email protected]/Lys-g-GO group than that in the PLGA and [email protected] groups (P < 0.05), suggesting that the collagen content of the new tissue was close to that of the normal bone tissue. The histological test results indicated that the implant of [email protected]/Lys-g-GO scaffold could achieve a good repair effect on bone defects, and the new tissue composition was close to that of the normal bone tissue.

Bio-Safety Assessment of Composite Scaffolds

Imaging and histological experiments confirmed that the Lys-g-GO and AuNPs-PDA functionalized coatings could significantly improve the bone repair ability of PLGA scaffolds. However, such nanomaterials could enter other parts of the body through body fluid circulation. Therefore, the bio-safety of scaffold materials also needs to be studied. At 12 weeks postoperatively, we conducted histopathological observation of the heart, liver, spleen, and kidney of rabbits. As shown in Figure 12, in the control group, HE results for heart tissue sections showed normal arrangement of myocardial fibre tissue, no significant changes in myocardial interstitium, and no granular degeneration, steatosis or other changes in myocardial cells. The results for liver HE stained sections showed that the structure of hepatic lobules was intact, hepatocytes were orderly arranged, and hepatocytes showed no degeneration or sclerosis. HE results for spleen tissue sections showed that the morphology of red pulp and white pulp was normal, and no significant increase of endothelial cells and polynucleated white blood cells was evident in the sinus. HE results showed a normal size and number of glomeruli, and there was no obvious infiltration of inflammatory cells in the renal interstitium. Compared with the normal rabbit viscera of the control group, no abnormal cell or structural changes were observed in the viscera of animals in all experimental groups, suggesting that the scaffold materials in each group did not cause significant damage to the visceral organs. The above experimental results indicated the excellent biocompatibility and bone repair ability of the [email protected]/Lys-g-GO composite porous scaffold prepared by us.

Figure 12 At 12 weeks after surgery, H&E staining sections of important organs (heart, liver, spleen and kidney) in experimental animals. PLGA (A), [email protected] (B), [email protected] (C), PLGA/Lys-g-GO (D) and [email protected]/Lys-g-GO (E). Scale bar lengths are 200 μm.

The treatment of large bone defects has always been the focus of orthopaedic research. Although therapeutic modalities such as autologous bone grafts are effective for bone regeneration, many drawbacks have limited their development, such as immune reactions, limited donor sources and transfer of pathogens. With the continuous development of tissue engineering technology, an increasing number of researchers have paid attention to the use of synthetic degradable polymers to solve the drawbacks existing in the traditional treatment of bone defects and promote the repair of bone defects. The ideal bone repair material should have good hydrophilicity, mechanical strength, antibacterial properties, and osteogenic induction ability, provide an appropriate environment for cell growth and proliferation, and promote the healing rate of bone tissue. This research employed Lys-g-GO nanoparticles and AuNPs-PDA coatings to optimize the physical and chemical properties of PLGA scaffolds and improve their tissue repair ability. The results showed that combining the AuNPs-PDA functionalized coating and Lys-g-GO nanoparticles could significantly improve the hydrophilicity, mechanical strength, and antibacterial properties of the PLGA scaffold. Lys-g-GO and AuNPs-PDA coatings exhibited synergistic effects on promoting cell proliferation, adhesion, osteogenic differentiation, and calcium deposition. The animal experiment results showed that [email protected]/Lys-g-GO composite porous scaffold presented an excellent bone repair ability, and the new bone formation and collagen deposition at the defect sites were effectively improved. Additionally, scaffold materials had good biocompatibility and had no obvious toxicity or side effects on the important organs (liver, spleen, kidney and heart) of rabbits. Therefore, we believe that the scaffold materials that we prepared have good clinical development prospects and may play an essential role in bone tissue regeneration medicine.

Conclusions

In this study, a AuNPs-PDA coating modified PLGA/Lys-g-GO composite scaffold with a loosened skeleton was fabricated. The scaffold has a channel-like pore structure and full 3D interconnection, and the surface of pores has a honeycomb-like structure composed of microvoids, which was suitable for cell infiltration and nutrient transport. The application of Lys-g-GO nanoparticles and AuNPs-PDA coating markedly enhanced the hydrophilicity, mechanical properties and antibacterial properties of the PLGA scaffold, resulting in good osteogenic activity. In vivo results demonstrated that the [email protected]/Lys-g-GO composite scaffolds were effective in repairing rabbits radial defect. Successful regeneration of bone tissue was confirmed by increased mineralized collagen deposition and bone content. This study demonstrated that simultaneously using Lys-g-GO nanoparticles and AuNPs-PDA coating for functional modification of bone implants is a promising strategy, and the [email protected]/Lys-g-GO composite scaffold shows promise use in clinical applications for the treatment of bone defects.

Data Sharing Statement

The raw data used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Ethics Approval and Consent to Participate

Animals use and care were approved with the Institutional Animal Care and Use Committee of Jilin University, China (Ethical Approval No. 2019132) and were performed in accordance with the university’s guidelines. Furthermore, all animal experiments comply with the National Institutes of Health guide for the care and use of laboratory animals (NIH Publications No. 8023, revised 1978).

Acknowledgments

This work was supported by the National Major Program of New Drug Research and Development; Jilin Provincial Science and Technology Agency Foundation, Key Research and Development Project (20200404137YY); Jilin Province Educational Science Research Project (JJKH20211168KJ); Jilin Provincial Development and Reform Commission Project (2019C051-6); Specialized Direct Subordinated Program from Finance Department of Jilin Province (Grant No. 2020SCZT065).

Disclosure

There are no conflicts of interest to declare.

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