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Murine Macrophage Requires CD11b to Recognize Talaromyces marneffei

Authors Hu Y, Lu S, Xi L

Received 5 November 2019

Accepted for publication 10 March 2020

Published 27 March 2020 Volume 2020:13 Pages 911—920

DOI https://doi.org/10.2147/IDR.S237401

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Professor Suresh Antony


Yongxuan Hu,1– 3 Sha Lu,1 Liyan Xi1,4,5

1Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of China; 2Department of Dermatology and Venereology, The 3rd Affiliated Hospital of Southern Medical University, Guangzhou, People’s Republic of China; 3Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Guangzhou, People’s Republic of China; 4Dermatology Hospital of Southern Medical University, Guangzhou, People’s Republic of China; 5Department of Dermatology, Guangzhou First People’s Hospital, The Second Affiliated Hospital of South China University of Technology, Guangzhou, People’s Republic of China

Correspondence: Yongxuan Hu
Department of Dermatology and Venerology, The 3rd Affiliated Hospital of Southern Medical University, 183 West Zhongshan Road, Guangzhou 510630, People’s Republic of China
Tel/Fax +86 20 62784560
Email huyongxuan2003@163.com
Liyan Xi
Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat–sen University, 107 West Yanjiang Road, Guangzhou 510120, People’s Republic of China
Tel/Fax +86 20 81332289
Email xiliyan@mail.sysu.edu.cn

Introduction: Talaromyces marneffei (T. marneffei) is an emerging pathogenic fungus. Macrophage-1 antigen (Mac-1, CR3, CD11b/CD18) is an important receptor on innate immune cells and can recognize pathogens. However, the importance of CR3 in phagocytosis of T. marneffei by macrophages and their responses to T. marneffei have not been clarified.
Methods: We show that interaction of mouse peritoneal macrophages (pMacs) or RAW264.7 macrophages with T. marneffei of its conidia spores and yeast cells enhances CR3 expression on macrophages. The phagocytosis rate was determined using flow cytometry, RT-PCR and Western blotting were used to detect CD11b expression, and the levels of IFN-γ, TNF-α, IL-2, IL-4, IL-6 and IL-10 in the co-culture supernatants were determined by ELISA.
Results: Incubation of mouse macrophages with T. marneffei promoted phagocytosis of T. marneffei, which was dramatically mitigated by pretreatment with anti-CD11b antibody or knockdown of CR3 expression on macrophages. Then, interferon γ, tumor necrosis factor α, IL-4, IL-10 and IL-12 production in macrophages incubation with heat-killed T. marneffei was detected. CD11b expression on mouse macrophages was upregulated by T. marneffei. Incubation of T. marneffei promoted phagocytosis of T. marneffei by macrophages and high levels of pro-inflammatory and anti-inflammatory cytokine production by macrophages, which were mitigated and abrogated by pre-treatment with anti-CD11b or knockdown of CD11b expression.
Conclusion: These data indicated that murine macrophage requires CD11b to recognize Talaromyces marneffei and their cytokine responses to heat-killed T. marneffei in vitro.

Keywords: Talaromyces marneffei, CD11b, macrophage
 

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