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Multilocus sequence typing and variations in the oprD gene of Pseudomonas aeruginosa isolated from a hospital in China

Authors Liu H, Kong W, Yang W, Chen G, Liang H, Zhang Y

Received 20 September 2017

Accepted for publication 30 October 2017

Published 8 January 2018 Volume 2018:11 Pages 45—54

DOI https://doi.org/10.2147/IDR.S152162

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 2

Editor who approved publication: Professor Suresh Antony


Huiqin Liu,1 Weina Kong,1 Weina Yang,2 Gukui Chen,1 Haihua Liang,1 Yani Zhang1

1College of Life Sciences, Northwest University, Shaanxi, China; 2Department of Clinical Laboratory, The Children’s Hospital of Xi’an City, Shaanxi, China

Objectives: To provide information about the genetic relationships and mechanism underlying carbapenem resistance in Pseudomonas aeruginosa clinical isolates of a hospital in China.
Materials and methods: One hundred and sixty P. aeruginosa strains were isolated from a hospital in China. Susceptibility to 14 antimicrobial agents was determined by antimicrobial susceptibility testing. Multilocus sequence typing was used to characterize the genetic backgrounds of these clinical isolates. Forty-five strains were randomly selected for further evaluation of their carbapenem resistance mechanism. Their oprD gene was compared with the PAO1 sequence.
Results: Multilocus sequence typing analysis demonstrated that these isolates were highly diverse; 68 sequence types were identified, of which 28 were novel sequence types. Polygenic and eBURST analysis demonstrated genetically similar clones with dissimilar resistance profiles. Among the 45 randomly selected strains associated with carbapenem resistance, 2 were metallo β-lactamase producers; all the 45 strains were not AmpC overproducers. Sequence analysis revealed a high diversity in the oprD sequences among isolates. Strains susceptible to imipenem and meropenem with shortened L7 and L8 loops in oprD were the major strain types observed in this hospital.
Conclusion: This study indicated that oprD provided the main mechanism for carbapenem resistance. The shortened L7 and L8 loops are responsible for carbapenem susceptibility.

Keywords: Pseudomonas aeruginosa, multilocus sequence typing, carbapenem resistance, L7 and L8 loops in OprD

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