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Molecular Relatedness of Salmonella enterica Typhimurium Isolates from Feces and an Infected Surgical Wound

Authors Qin H, Guo Y, Li Y, Zheng R

Received 28 February 2020

Accepted for publication 16 June 2020

Published 6 July 2020 Volume 2020:13 Pages 2139—2144


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Suresh Antony

Haiyan Qin, 1,* Yidan Guo, 2,* Yikun Li, 3, 4 Rui Zheng 3, 4

1Department of Infection Prevention and Control, The First People’s Hospital of Kunming City, Kunming, Yunnan, People’s Republic of China; 2Yunnan Provincial Center of Disease Control and Prevention, Kunming, Yunnan, People’s Republic of China; 3Department of Clinical Laboratory, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China; 4Department of Clinical Laboratory, the First People’s Hospital of Yunnan Province, Kunming, Yunnan, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Rui Zheng Tel +86 871-63638430

Purpose: Salmonella enterica serovar Typhimurium infection is common in foodborne diseases, but its isolation from surgical incisions is rare. Our aim in this study was to trace the transmission source of a surgical incision infected with S. Typhimurium in a Yunnan Province hospital patient and elucidate the underlying molecular mechanisms of antibiotic resistance.
Methods: Primers were designed to amplify the drug-resistance genes using polymerase chain reaction (PCR). Susceptibility to antibiotics was determined using Etest strips. Macrorestriction profiles were analyzed using pulsed-field gel electrophoresis (PFGE) and XbaI. The two isolates were characterized using agglutination tests and multilocus sequence typing (MLST).
Results: MLST analysis revealed that S. Typhimurium isolates SM043 and SM080 belonged to the same genotype, ST34, and PFGE revealed that SM043 and SM080 had high similarity. The isolates were both resistant to third-generation cephalosporins. SM043 harbored the antibiotic resistance genes blaCTX-M-15, blaTEM-1, qnrS-1, qnrB, and acc-3, whereas blaCTX-M-15, blaTEM-1, blaCMY-2, qnrS-1, and acc-3 were detected in SM080.
Conclusion: The surgical incision infection by S. Typhimurium may have been hospital-acquired. Thus, it is critical to strengthen hospital sanitation by addressing hand hygiene and sterilization of the operational environment to avoid outbreaks of nosocomial Salmonella infections.

Keywords: Salmonella, healthcare-associated infection, cephalosporins, antibiotic resistance, nosocomial infection

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