miR-29a inhibits proliferation, invasion, and migration of papillary thyroid cancer by targeting DPP4
Authors Wang Y, Han J, Lv Y, Zhang G
Received 14 January 2019
Accepted for publication 15 April 2019
Published 28 May 2019 Volume 2019:12 Pages 4225—4233
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Jyoti Bajaj
Peer reviewer comments 2
Editor who approved publication: Dr Federico Perche
Yufei Wang,* Jie Han,* Yuetao Lv, Guochao Zhang
Department of Breast and Thyroid Surgery, Jining NO.1 People’s Hospital, Affiliated Jining NO.1 People’s Hospital of Jining Medical University, Jining Medical University, Jining City, Shandong Province 272011, People’s Republic of China
*These authors contributed equally to this work
Purpose: The purpose of this study was to investigate the effects of miR-29a on papillary thyroid cancer (PTC) and its underlying mechanisms.
Methods: Primary tumor tissues and adjacent tissues of 69 patients with PTC were obtained. Human thyroid cell line Nthy-ori3-1 and PTC cell lines K1, BCPAP, TPC-1 were cultured. K1 cells were transfected and divided into following groups: blank group (without any treatment), miR-29a mimics group, control mimics group, miR-29a inhibitor group, control inhibitor group, DPP4 siRNA group, control siRNA group and miR-29a inhibitor + DPP4 siRNA group. qRT-PCR and Western blot were used to detect miR-29a and DPP4 expression. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and transwell assay were performed to detect cells proliferation, migration, and invasion. A nude mice xenograft experiment was performed.
Results: miR-29a was significantly downregulated in PTC tissues, K1 and TPC-1 cells (P<0.01). DPP4 was significantly upregulated in the miR-29a inhibitor group and significantly downregulated in the miR-29a mimics group (P<0.01). DPP4 was the target gene of miR-29a. miR-29a significantly inhibited K1 cell proliferation, invasion, migration and PTC growth in nude mice by targeting DPP4 (P<0.01).
Conclusion: miR-29a inhibits proliferation, migration, and invasion of PTC by targeting DPP4, which might provide a new target for clinical treatment of PTC.
Keywords: PTC, miR-29a, DPP4, proliferation, migration
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