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miR-204-5p acts as a tumor suppressor by targeting matrix metalloproteinases-9 and B-cell lymphoma-2 in malignant melanoma

Authors Luan WK, Qian Y, Ni X, Bu XF, Xia Y, Wang JL, Ruan HR, Ma SJ, Xu B

Received 27 November 2016

Accepted for publication 3 January 2017

Published 27 February 2017 Volume 2017:10 Pages 1237—1246

DOI https://doi.org/10.2147/OTT.S128819

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Amy Norman

Peer reviewer comments 2

Editor who approved publication: Dr Ingrid Espinoza

Wenkang Luan,1,* Yao Qian,2,* Xin Ni,3,* Xuefeng Bu,4 Yun Xia,1 Jinlong Wang,1 Hongru Ruan,1 Shaojun Ma,1 Bin Xu1

1Department of Plastic Surgery, 2Department of Neurosurgery, 3Department of Gastroenterology, 4Department of General Surgery, Affiliated People’s Hospital of Jiangsu University, Zhenjiang, Jiangsu, People’s Republic of China

*These authors contributed equally to this work

Abstract: An increasing number of microRNAs have been found to be involved in tumorigenesis, including melanoma tumorigenesis. miR-204-5p is down-regulated and functions as a tumor suppressor in many human malignant tumors. miR-204-5p expression is also decreased in melanoma tissues, but its biological roles and molecular mechanisms in malignant melanoma remain unclear. In this study, the aberrant down-regulation of miR-204-5p was detected in melanoma, especially in metastatic melanoma. miR-204-5p also served as a protective factor for the prognosis of melanoma patients. We determined that miR-204-5p suppresses cell proliferation, migration and invasion, and promotes cell apoptosis in melanoma. Matrix metalloproteinases-9 and B-cell lymphoma-2 are the functional targets of miR-204-5p, through which it plays an important biological role in malignant melanoma. The effect of miR-204-5p on malignant melanoma is verified using a xenograft model. We also determined that miR-204-5p increases 5-fluorouracil and cisplatin (DDP) chemosensitivity in malignant melanoma cells. This finding elucidates new functions and mechanisms for miR-204-5p in melanoma development, and provides potential therapeutic targets for the treatment of melanoma.

Keywords: miR-204-5p, MMP9, BCL2, melanoma, cell apoptosis, migration, invasion

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