microRNA-582 Potentiates Liver and Lung Metastasis of Gastric Carcinoma Cells Through the FOXO3-Mediated PI3K/Akt/Snail Pathway
Authors Xie T, Wu D, Li S, Li X, Wang L, Lu Y, Song Q, Sun X, Wang X
Received 11 January 2020
Accepted for publication 30 May 2020
Published 30 June 2020 Volume 2020:12 Pages 5201—5212
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Eileen O'Reilly
Tianyu Xie,1,2 Di Wu,2 Shuo Li,1,2 Xiongguang Li,1,2 Lipeng Wang,2 Yixun Lu,2 Qiying Song,2 Xuehong Sun,2 Xinxin Wang1,2
1School of Medicine, Nankai University, Tianjin 300071, People’s Republic of China; 2Department of General Surgery, Chinese PLA General Hospital, Beijing 100853, People’s Republic of China
Correspondence: Xinxin Wang Tel/ Fax +86-10-66938328
Background: The dysregulation of microRNA (miRNAs) is broadly participated in cancer progression, resulting in sustained cell proliferation by directly targeting various targets. This study investigated the expression of miR-582 in GC and its association with liver metastasis.
Methods: Firstly, differentially expressed miRNAs in gastric cancer (GC) tissues were predicted by microarray. Then, the relationship between miR-582 and clinical characteristics of GC patients was analyzed. By silencing of miR-582 in GC cells, the change in malignant biological behaviors of GC cells was detected. The upstream lncRNA, downstream targeting genes of miR-582 and the corresponding signaling pathway were predicted by online databases and verified by luciferase reporter assays, RT-qPCR and Western blot analysis. Finally, the effects of miR-582 on the growth and metastasis of GC cells were detected by in vivo tumorigenesis and metastasis tests.
Results: MiR-582 was highly expressed in GC tissues and related to the metastasis of patients with GC. Silencing of miR-582 expression blocked malignant biological behaviors of GC cells in vitro and in vivo. MiR-582 inhibited forkhead box protein O3 (FOXO3) to upregulate the PI3K/AKT/Snail signaling pathway in GC cells. Besides, GATA6-AS1 was found as an upstream lncRNA to modulate the expression of miR-582.
Conclusion: MiR-582 induced by GATA6-AS1 silencing promotes the growth and metastasis of GC cells by targeting FOXO3 to induce the activation of the PI3K/AKT/Snail signaling pathway. MiR-582 could be a potential molecular therapy target for patients with GC.
Keywords: gastric cancer, miR-582, PI3K/AKT/Snail signaling pathway, proliferation, metastasis, tumorigenesis
Gastric carcinoma (GC) contributes to over 8% of the total new diagnose and 10% of total deaths associated with cancer, with more than 70% of new cases and deaths occurring in under-developed countries.1 The risk factors for GC include H. pylori infection, unhealthy diet, smoking history as well as alcohol consumption.2 Despite significant progress has been made in surgical treatments and novel chemotherapy protocol development, the overall clinical outcome for advanced GC sufferers is dismal (5–20% for a 5-year survival and 10 months for a median overall survival).3 Epidemiological evidence displays that the liver, peritoneum and lung rank the top three most common sites for metastases, a major cause of poor prognosis of GC.4 Additionally, limited metastasis-related biomarkers were substantiated and applied in clinic, as a consequence, the detection of new metastasis-related biomarkers for GC is urgently needed for risk prediction and GC metastasis treatment.5
MicroRNAs (miRNAs) are a class of non-coding RNAs with 18–25 nucleotides lengthen that repress translation or enhance messenger RNAs (mRNAs) degradation with complementary sequences, and several miRNAs, including miR-18a, miR-96, and miR-370 showed elevated expression in GC versus normal or adenoma samples.6 Accordingly, our microarray experiments provided data indicating a significant upregulation of miR-582 between GC tissues and paracancerous tissues, highlighting the possible involvement of miR-582 in GC. miR-582 was upregulated in colorectal cancer tissues and cell lines, which was associated with promoted proliferative and migratory ability of the colorectal cancer cells.7 Moreover, miR-582-5p suppressed monocyte apoptosis by reducing forkhead box protein O (FOXO) 1 expression and might function importantly in regulating tuberculosis progression.8 In the present study, we also predicted and verified a binding site between miR-582 and FOXO3 in GC cells, underscoring the interaction between miR-582 and FOXO3 in GC. Interestingly, the expression of FOXO3a was observed to be substantially lower in cancerous tissues relative to the adjacent non-tumorous tissues, which was tightly linked to lymph node metastasis and unsatisfactory prognosis for GC patients.9 In addition, the phosphatidylinositol-3 kinase (PI3K)/protein kinase B (Akt)/β-catenin pathway modulated the transcription of Vestigial-like 1, thus potentiating the proliferation and metastasis in GC.10 More importantly, the PI3K/Akt pathway represents one of the most vital pathways interacting with FOXOs in different types of tumors.11 miR-582, the most upregulated miRNAs, was selected in the current study to find its binding target genes. Long non-coding RNAs (lncRNAs), a group of transcripts larger than 200 nt in length, exhibit promising values as biomarkers for GC diagnosis and prognosis determination.1–12 Moreover, GATA6 antisense RNA 1 (GATA6-AS1) has been indicated to have the highest lung squamous cell carcinoma diagnostic values.2–13 However, its connection with miR-582 in GC remains unclear. Therefore, in vivo and in vitro experiments were carried out to explore the effects of the lncRNA/miRNA/target gene network on the PI3K/Akt/Snail pathway, as well as the epithelial-mesenchymal transition (EMT), proliferation, migration and invasion of GC cells in vitro and metastasis in vivo. Our findings revealed a novel mechanism regarding miR-582 in GC progression.
Patients and Methods
Patients and Tumor Tissue Samples
Forty-five malignant GC tissues along with their matched adjacent tissues, with a distance of > 3 cm cm from the GC tissues were attained from patients undergone surgery at the Chinese PLA General Hospital from July 2013 to January 2014. There were 25 males and 20 females (average age 52.62 ± 10.06 years). Patients that met the eligibility criteria were enrolled: 1) diagnosed as GC by histopathological examinations; 2) no treatment, including chemotherapy or radiotherapy received before the surgery; 3) accessibility of comprehensive clinical data; 4) no previous history of other malignancies or chronic systemic diseases. The clinicopathological information of the 45 GC patients was listed in Supplementary Table 1.
Microarray Experiments and Data Processing
Six GC tissues were applied to extract 0.5 μg total RNA to synthesize cDNA using the GeneChip 3ʹInvitro Transcription (IVT) Express Kit (902,789, Thermo Fisher Scientific Inc., Waltham, MA, USA) as per the supplier’s instructions. Subsequently, cDNA was segmented and hybridized with human miRCURY LNA™ Universal RT microRNA PCR Human panel. After hybridization, the arrays were scanned with GeneChipTM Scanner 3000 7G system (000213, Thermo Fisher Scientific).
Reverse Transcriptase Polymerase Chain Reaction and Quantitative Real-Time PCR (RT-qPCR)
The total RNA was isolated from tissues and cells with the application of TRIzol reagent (Invitrogen, Carlsbad, CA, USA). The primers used in the experiment including GATA6-AS1, miR-582, U6, FOXO3 and GAPDH were from Applied Biosystems (ABI, Foster City, CA, USA). ABI 7500 was utilized to performed the qPCR work.
Cell Culture and Treatment
The GES-1 (immortalized gastric mucosa epithelial cells) and the BGC-823, MKN-45, HGC-27, SGC-7901 and MGC-803 (GC cells) were all purchased from Cell Bank of Shanghai Institute of Cells, Chinese Academy of Science (Shanghai, China). After being plated in cell culture dishes at 1 × 105 cells/cm2, the cells were grown in Dulbecco’s modified Eagle’s medium or Roswell Park Memorial Institute-1640 medium containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA) for 48 h with 5% CO2 at a temperature of 37°C. When cells were covered with 80–90% microscopic view, the trypsinized cells were sub-cultured.
The BGC823 cells and MKN-45 cells with good growth were transfected with the synthesized miR-582 inhibitor and the corresponding inhibitor control, respectively. The Lipofectamine™ 3000 system (Invitrogen, Carlsbad, CA, USA) was applied for viral transfection.
Cell Viability Assays
The viabilities of cells were examined by 5ʹ-ethynyl-2ʹ-deoxyuridine (EdU) staining as described by Yang et al.14 The cell proliferation activity was detected using an EdU staining proliferation kit (ab222421, Abcam). In general, cells in good growth conditions were incubated for 2 h at 37°C with the staining solution in the kit. After counter-staining of the nucleus with 4ʹ,6-Diamidino-2-Phenylindole (DAPI), the number of EdU positive cells in each well was counted in three different visual fields. The apoptosis level of GC cells was detected by Hoechst 33,258 staining as previously reported.15 In short, apoptotic cells in each well were counted in three different visual fields. The number of apoptotic cells in each field was divided by the total number of cells in field as the apoptotic rate.
The expression of EMT markers in GC cells was detected by immunofluorescence. The frozen sections (5-μm in thickness) were fixed in acetone and sealed with 1% bovine serum albumin (BSA) in the phosphate buffered saline (PBS). The antibodies against N-cadherin (1:1000, ab18203, Abcam Inc., Cambridge, UK) and E-cadherin (1:100, ab48394, Abcam) were used for an overnight staining at 4°C. The secondary antibody to rabbit (1:5000, ab150088, Abcam) labeled with fluorescein isothiocyanate was incubated for 1 h at 37°C. Following blocking endogenous biotin using a streptavidin-biotin blocking kit (Vector Laboratories, Inc., Burlingame, CA, USA), the cells were then counter-stained with DAPI and visualized with a fluorescence microscope (Leica DM 3000, Leica Microsystems GmbH, Wetzlar, Germany).
Transwell Assay for Migration and Invasion
Cell invasion and migration were tested by Transwell assay in strict accordance with the previous literature.16 The apical chamber of the Transwell plate was coated with 50 μL Matrigel (BD Biosciences, Franklin Lakes, NJ, USA). The cells (5 × 104) were suspended in 100 μL serum-free medium and then seeded into the upper layer of the Transwell chamber (BD Biosciences). Totally 500 μL medium containing 20% fetal bovine serum was added to the basolateral chamber. After 48 h at 37°C with 5% CO2, cells retained in the apical chambers were scratched. The cells in the basolateral chamber were fixed in methanol, stained in 0.1% crystal violet and photographed under an inverted contrast microscope (CK2, Olympus, Japan). For the migration experiment, the apical chamber was free of Matrigel, and the remaining operations were consistent with the invasion experiment.
Luciferase Reporter Assays
The 3ʹuntranslated region (UTR) binding sequences of miR-582 and GATA6-AS1 as well as FOXO3 were predicted by online prediction softwares RNA22, StarBase and DIANA. FOXO3 or GATA6-AS1 wild type (WT) and 3ʹUTR binding sequence mutant (MT) were synthesized by a commercial company Shanghai Sangon Biological Engineering Technology & Services Co., Ltd. (Shanghai, China) and inserted into pMIR-REPORTTM (Thermo Fisher Scientific). The engineered luciferase reporter plasmids were delivered with miR-582 mimic or miRNA NC into 293T cells using the Lipofectamine™ 3000 kit (Invitrogen) following the supplier’s instructions. The relative luciferase activity was evaluated after 24 h with the Dual-Luciferase Reporter Assay System (Promega Corporation, Madison, WI, USA).
The cells were lysed on ice in radio immunoprecipitation assay lysis buffer containing a mixture of proteinase and phosphatase inhibitors for 30 min and then centrifuged at 13,000 g for 10 min. The protein concentration was subsequently measured using a bicinchoninic acid kit. A total of 20 μg protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred onto a polyvinylidene difluoride membrane. Subsequently, the membranes were blocked with 5% BSA and incubated with the diluted primary antibodies against pFOXO3S253 (1:2000, ab47285, Abcam), PI3K (1:5000, ab1549, Abcam), pPI3KY607 (1:5000, ab182654, Abcam), AKT1 (1:5000, ab235958, Abcam), pAKT1S473 (1:50, ab812, Abcam) and Snail (1:50, ab53519, Abcam) at 4°C overnight. After 3 rinses with Tris-buffered saline Tween-20 (TBST), the membranes were incubated with the horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody (1:10,000, ab7090, Abcam) for 1 h. After 3 rinses with TBST, the membranes were visualized using a SuperSignal West Pico Kit (Thermo Fisher Scientific Inc). Images were captured using the ChemiDoc™ XRS (Bio-Rad, Inc., Hercules, CA, USA). β-actin was regarded as the loading control. The expression of target protein = gray value of target protein bands/gray value of β-actin.
In total, 24 mice (aged 4–6 weeks; weight, 20 ± 2g, Beijing Vital River Laboratory Animal Technology Co., Ltd., Beijing, China) were used. HepG2 and SMMC7721 cells (4 × 106) transfected with miR-582 inhibitor or mock in 2 mL saline were injected into BALB/c nude mice subcutaneously (n = 6). After 7 d of injection, the tumor size was examined every 7 d for 28 d totally. Tumor volume (V) was evaluated by measuring the length (m2) and width (m1) with caliper and calculated with the formula V = m1 2 × m2 × 0.5236. By the end of the experiment, the nude mice were euthanized with overdose of pelltobarbitalum natricum at 120 mg/kg. The tumor was harvested for histological examination.
The number of KI67 positive cells in tumor sections was detected using immunohistochemistry as previously reported.17 Briefly, tumor tissue sections were deparaffinized, rehydrated, and then incubated at 95°C in citrate buffer for 20 min for antigen retrieval. At room temperature, nonspecific binding was blocked with 10% normal goat serum for 30 min. The sections were cultured with the primary antibody to KI67 (1:1000, ab15580; Abcam) at 4°C overnight and with HRP-conjugated goat anti-rabbit secondary antibody to IgG (1:500; ab6721; Abcam) at 30°C for 30 min. At room temperature, the sections were then reacted with the peroxidase coupled with the antibiotic streptomycin at room temperature for 30 min. The reaction products were visualized using a diaminobenzidine kit (Beijing Solabio Life Sciences Co., Ltd., Beijing, China) and quantitatively analyzed by NIH Image J software (Bethesda, MD, USA).
Tail Vein Injections into Nude Mice
The nude mice were injected with cells (4 × 106) stably transfected with miR-582 inhibitor or mock via the tail veins, which were euthanized 30 or 45 d after injection with pelltobarbitalum natricum. The lungs and liver were removed to conduct the hematoxylin and eosin (HE) staining for further analysis.
The results were all analyzed using SPSS version 21.0 software (SPSS, Inc., Chicago, IL, USA). The normal distribution of data was tested using Kolmogorov-Smirnov and represented by mean ± standard deviation (SD). Pairwise differences between two groups were analyzed using unpaired t-test. One-way or two-way analysis of variance (ANOVA) with Tukey’s post hoc test were used for evaluating the difference among multiple groups. Fisher’s exact test was applied to analyze the association of enumeration data. Data were considered statistically significant if p value less than 0.05.
The High Expression of miR-582 in GC Tissues Is Related to Metastasis in GC
First, we used the microRNA microarray to detect the cancer tissues and adjacent normal tissues from 5 patients with GC. By using log FoldChange > 2, adjust p < 0.05 as the screening condition, we screened out 695 differentially expressed miRNAs, of which 396 were up-regulated and 299 were down-regulated. The heatmap shows the top 30 differentially expressed miRNAs in Figure 1A. Then, we detected the expression of miR-582, miR-493, miR-338, miR-1254, miR-34a and miR-654 in 45 GC tissues and paracancerous tissues by RT-qPCR, and observed that miR-582 was the most enhanced one in GC tissues (Figure 1B). Also, we monitored that the miR-582 expression in tissues from GC patients with liver and lung metastases was remarkably promoted versus GC patients free of liver, lung and lymph node metastases (Figure 1C–E). Subsequently, we tested the expression of miR-582 in GC cell lines and GES-1 cells, and found that the miR-582 was drastically enhanced in the GC cell lines, and the promotion of that was more pronounced in BGC-823 and MKN-45 cells (Figure 1F). Therefore, in order to verify the effect of miR-582 on the GC cell malignant potentials, we transfected miR-582 inhibitor into BGC-823 and MKN-45 cells. The transfection efficiency was tested by RT-qPCR (Figure 1G). Together, our findings show that miR-582 is promoted in GC tissues and cell lines.
miR-582 Enhances the Malignant Potentials of GC Cells
The results of EdU assay and Hoechst 33,257 staining exhibited that inhibition of miR-582 expression reduced cell proliferation and viability and even promoted apoptosis (Figure 2A and B). The immunofluorescence staining of EMT makers E-cadherin and N-cadherin demonstrated that the decrease of miR-582 weakened the EMT of GC cells (Figure 2C). Then, we carried out Transwell experiments to test the cell invasion and migration (Figure 2D and E), and it was found that the BGC-823 and MKN-45 cell migration and invasion were inhibited after the treatment with miR-582 inhibitor. These data propose that miR-582 inhibitor diminishes the proliferation, invasion, migration and EMT abilities of the GC cells.
miR-582 Facilitates Malignant Phenotypes in GC by Binding to FOXO3
In order to clarify the downstream mechanism of miR-582, the target genes of miR-582 were predicted and screened out by StarBase, TargetScan and miRSearch. We screened out a large number of genes, in which FOXO3 drew our attention. Furthermore, miR-96-5p can target FOXO3 to promote the growth of GC cells.18 Thus, we examined FOXO3 expression in cancer tissues of 45 GC sufferers (Figure 3A). FOXO3 was suggested to be significantly downregulated and negatively correlated with miR-582 expression (Figure 3B). Subsequently, we found that the FOXO3 expression in cancer tissues of GC patients with lung and liver metastases was markedly reduced relative to GC patients without liver, lung and lymph node metastases (Figure 3C–E).
Therefore, the target binding relationship between miR-582 and FOXO3 was detected by luciferase report assays (Figure 3F). Besides, miR-582 could target FOXO3 directly in GC cells, as the FOXO3 expression at both mRNA and protein levels in BGC-823 and MKN-45 cells found to be increased by miR-582 inhibitor (Figure 3G and H). These data imply that miR-582 induces the progression of GC by interacting with FOXO3.
miR-582 Promotes Invasion and Migration of GC Cells by Activating the PI3K/Akt/Snail Pathway Through FOXO3
A previous study pointed out that FAM83A induced the EMT of non-small cell lung cancer by potentiating the PI3K/Akt/Snail pathway, thereby promoting the metastasis of lung cancer.19 Thus, we used immunoblotting to detect the protein expression of PI3K/Akt/Snail and the extent of PI3K/Akt/Snail phosphorylation in BGC-823 and MKN-45 cells treated with miR-582 inhibitor or mock. As shown Figure 4A, pPI3K and pAKT1 were significantly inactivated in miR-582 inhibitor-treated GC cells.
Furthermore, in order to verify the role of the PI3K/Akt/Snail pathway in miR-582-mediated proliferation and metastasis, we used a PI3K-specific agonist P740Y-P on miR-582 inhibitor-transfected cells. Decreased viability, EMT, migration and invasion of BGC-823 and MKN-45 cells and increased apoptosis by miR-582 inhibitor were reversed by P740Y-P (Figure 4B–E).
miR-582 Potentiates Tumor Growth and Metastasis in vivo
To validate the effects of miR-582 in vivo, BGC-823 and MKN-45 cells treated with mock or miR-582 inhibitor were subcutaneously injected into nude mice. The tumor volume growth curve and the weight columns revealed that growth rate of the GC cells was slowed by miR-582 inhibitor (Figure 5A and B). Subsequently, we used the immunohistochemistry to detect the number of KI67 positive cells in the tumor, and we found that the downregulation of miR-582 repressed the rate of positive cells in the tumor (Figure 5C).
To directly verify the role of miR-582 in metastasis in vivo, we conducted a separate analysis in nude mice by injecting stable miR-582-knocked down BGC-823 and MKN-45 cells in the tail vein. The mice injected with stable miR-582-depleted cells had markedly fewer metastases in lung and liver than that of mice injected with stable mock-depleted cells (Figure 5D). The promotive effect of miR-582 on lung and liver metastasis of GC was further confirmed in vivo.
GATA6-AS1 Regulates miR-582 Expression in GC Cells
We subsequently screened five lncRNAs on the upstream of miR-582, NEAT1, KCNQ1OT1, MIR17HG, GATA6-AS1 as well as PRKCQ-AS1 (Figure 6A) based on RNA22, StarBase and DIANA bioinformatics prediction websites. Moreover, we predicted the expression of the five lncRNAs in stomach adenocarcinoma (STAD) as well as in normal tissues through GEPIA websites. We observed that only NEAT1 and GATA6-AS1 had significant differences (Figure 6B). GATA6-AS1 overexpression has been reported in a literature to significantly inhibit GC proliferation and EMT.3–20 As a consequence, we used RT-qPCR to detect the expression of GATA6-AS1 in tumor tissues and paracancerous tissues in 45 GC patients, and found that GATA6-AS1 was significantly lower in GC tissues (Figure 6C). We subsequently verified the binding relationship between GATA6-AS1 and miR-582 by dual luciferase experiments. The luciferase activity in 293T cells decreased significantly after transfection of GATA6-AS1-WT and miR-582 mimic (Figure 6D). Given the aforementioned findings, we concluded that GATA6-AS1 negatively regulated the miR-582 expression in GC.
Comparable to other cancers, gene expression patterns are altered in GC, one of the most frequent cancers throughout the world, and two main categories of genes are participated in the progression of cancers: oncogenes with promoted expression and tumor suppressors with decreased expression.21 The crucial regulatory function of miRNAs as diagnostic and therapeutic biomarkers in cancers is gradually documented by sequence-specific targeting oncogenes or tumor suppressors.22 In the present study, we screened the dysregulated miRNAs in GC by microarray analysis and verified that miR-582 was the most upregulated one in 45 cases of GC tissues by RT-qPCR. Cell experiments showed that inhibition of miR-582 could profoundly enhance E-cadherin expression and diminish N-cadherin expression, thereby preventing GC cells from undergoing EMT and effectively attenuating the proliferation, migration and invasion abilities. Conversely, the PI3K specific agonist 740Y-P remarkably potentiated the proliferation, EMT, migration and invasion of GC cells. In addition, miR-582 was monitored to interact with GATA6-AS1 and FOXO3, respectively. Based on the abovementioned results, we believe that miR-582 acts as an oncogene in GC via the FOXO3-mediated PI3K/Akt/Snail pathway.
With the intention of identifying vital miRNAs as GC therapeutic biomarkers in the study, five GC tissues were obtained from healthy persons and GC patients for microarray screening analysis, from which 30 miRNAs were screened out the plot the heatmap. Our RT-qPCR results further demonstrated that miR-582 was upregulated in the GC patients and closely correlated to metastasis. It has been suggested that miR-582 expression was significantly promoted, mediating induction of the Wnt/β-catenin signaling pathway, and accelerating tumorigenesis and recurrence in non-small-cell lung carcinoma.23 Besides, miR-582-5p was overexpressed in clinical tissues and colorectal cancer cells relative to adjacent tissues isolated from the same patients.24 Moreover, the proliferation, migration and invasion of colorectal cancer cells were demonstrated to be induced by overexpression of miR-582, while miR-582 inhibition contributed to the opposite effect, indicating the oncogenic role of miR-582 in colorectal cancer.25 Through the online prediction databases, we found that FOXO3 was one of the unreported potential targets of miR-582. The dual luciferase activity reporter assay determined the targeting correlations between FOXO3, GATA6-AS1 and miR-582. Upregulated GATA6-AS1 suppressed GC cell proliferation, invasion and migration.3–20 Nevertheless, the interaction between GATA6-AS1 and miRNAs has never been investigated before. In the present work, we identified the binding relationship between GATA6-AS1 and miR-582, while further investigation regarding this lncRNA and the downstream gene was necessary.
Additionally, the loss of FOXO3 interfered the transcription of its targets, leading to downstream responses that might accelerate tumorigenesis.26 Western blot analysis conducted by He et al showed that only FOXO3, rather than FOXO1, FOXO4 or FOXO6, was reduced in cancer regions of GC patients,18 which is in accordance with our current finding. FOXO3 is a well-known modulator of the PI3K/Akt pathway in pancreatic cancer27 and hepatocellular carcinoma.28 Notably, tumor-associated macrophages facilitated the EMT process through the PI3K/Akt/Snail pathway, while impeding this axis with matrix metalloproteinase-9 proenzyme inhibitor repressed metastases in GC.29 Likewise, the PI3K/Akt/Snail pathway was probably linked to the enhancement of EMT through Aquaporin 3 in GC.30 In the current study, we verified that the PI3K specific agonist 740Y-P reversed the alteration of these cell activities mediated by miR-582. miR-582 could downregulate the expression of FOXO3 in GC cells, thereby inducing the activity of PI3K/Akt/Snail signaling transduction. Similar to our data, LINC02465 inhibition hampered the activation of the PI3K/Akt pathway, and the induction of this pathway through 740Y-P reversed the suppressive role of si-LINC02465 on the malignant phenotypes of GC cells.31 Many EMT-inducing transcription factors, including Snail have been linked to tumor invasion and metastasis.32 Therefore, repression of Akt or Snail counteracted the stimulative effect of family with sequence similarity 83, member A overexpression in metastasis of non-small cell lung cancer.19 Consistent with our in vitro results, miR-582 downregulation also inhibited the growth and distant metastasis of mouse xenografts in vivo. Immunohistochemical staining of the tissue sections disclosed that KI67 expression was profoundly reduced in mice treated with miR-582 downregulation.
In conclusion, we identified in the study that miR-582 was promoted in GC tissues and cells, which was associated with downregulated of GATA6-AS1. miR-582 could downregulate the expression of the target gene FOXO3 and increase the activity of PI3K/AKT/Snail signaling pathway, thus exerting a tumor-initiating effect on growth, invasion and metastasis of GC. Collectively, the GATA6-AS1/miR-582/FOXO3/PI3K/AKT/Snail axis may have potential as a target for GC treatment and deserves further researches.
Ethics Approval and Consent to Participate
This study was permitted and supervised by the ethics committee of the Chinese PLA General Hospital. All the enrolled subjects provided written informed consents. The protocol was accepted by the Institutional Animal Care and Use Committee of the Chinese PLA General Hospital as well. All experiments involving animals were carried out following the Guide for the Care and Use of Laboratory animals published by the National Institutes of Health. Noteworthy efforts were made to curtail the number of animals and their sufferings.
All authors made substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data; took part in drafting the article or revising it critically for important intellectual content; gave final approval of the version to be published; and agree to be accountable for all aspects of the work.
The authors declare no potential conflicts of interest in this work.
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