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MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats

Authors Li S, Ma H, Yuan X, Zhou X, Wan Y, Chen S

Received 27 December 2019

Accepted for publication 17 August 2020

Published 9 September 2020 Volume 2020:16 Pages 2053—2061

DOI https://doi.org/10.2147/NDT.S243920

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Yuping Ning


Shuqian Li,1 Hong Ma,2 Xu Yuan,1 Xiaomei Zhou,1 Yiwen Wan,1 Shangjie Chen1

1Department of Rehabilitation, People’s Hospital of Shenzhen Baoan District, Shenzhen 518100, People’s Republic of China; 2Department of Rehabilitation, Binzhou Medical University, Yantai, Shandong Province 264003, People’s Republic of China

Correspondence: Shangjie Chen
Department of Rehabilitation, People’s Hospital of Shenzhen Baoan District, 118 Longjing Second Road, Xin’an Street, Shenzhen, Baoan District 518100, People’s Republic of China
Email nx7805@163.com

Background: Depression is an emotional disorder characterized by depression, lack of pleasure, and cognitive and sleep disorders. It is a systemic disease with a complex pathogenesis. In this study, we will be focused to investigate their associations and the exact functional mechanisms of miR-382-5p and NR3C1 in depression.
Materials and Methods: We measured the expressions of microRNA-382-5p (miR-382-5p) and NR3C1 in the hippocampus by chronic unpredictable mild stress (CUMS). Depression behavior test including novelty-suppressed feeding test (NSFT), sucrose preference test (SPT), and forced swim test (FST) on rats have been conducted to examine the roles and functions of miR-382-5p and NR3C1 on depression-like behaviors by lentivirus vectors.
Results: Up-regulation of miR-382-5p and down-regulation of NR3C1 were observed in rats’ hippocampus induced by CUMS. miR-382-5p targeted NR3C1 and inhibited the expressions of NR3C1 in rats’ hippocampus. miR-382-5p could significantly change the depression behaviors induced by CUMS. NR3C1 downstream BDNF and p-TrkB were also oppositely associated with miR-382-5p in rats’ hippocampus.
Conclusion: Through our experiments and analysis, we found that the associations between miR-382-5p and NR3C1 could affect the depression-like behaviors.

Keywords: miRNA-382-5p, depression, hippocampus, NR3C1

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