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Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression

Authors Dai J, Wei R, Zhang P, Liu P

Received 13 November 2019

Accepted for publication 18 December 2019

Published 23 January 2020 Volume 2020:12 Pages 567—580

DOI https://doi.org/10.2147/CMAR.S238373

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Eileen O'Reilly


This paper has been retracted.

Jun Dai, 1 Rujia Wei, 2 Peihai Zhang, 3 Peishu Liu 1

1Department of Gynaecology and Obstetrics, Qilu Hospital of Shandong University, Jinan, Shandong 250012, People’s Republic of China; 2School of Life Sciences, Liaocheng University, Liaocheng, Shandong 252004, People’s Republic of China; 3Department of Gynaecology and Obstetrics, Qilu Hospital of Shandong University (Qingdao), Qingdao, Shandong 266035, People’s Republic of China

Correspondence: Jun Dai
Department of Gynaecology and Obstetrics, Qilu Hospital of Shandong University, 107 Wenhua West Road, Jinan, Shandong 250012, People’s Republic of China
Email daijun_qilu@163.com
Peihai Zhang
Department of Gynaecology and Obstetrics, Qilu Hospital of Shandong University (Qingdao), 758 Hefei Road, Qingdao, Shandong 266035, People’s Republic of China
Email doctorzhang_ph@163.com

Purpose: A long noncoding RNA called ZFPM2 antisense RNA 1 (ZFPM2-AS1) has been verified as a key modulator in multiple human cancer types. Nonetheless, the expression and functions of ZFPM2-AS1 in cervical cancer remain poorly understood. Therefore, our purpose was to characterize the expression pattern, clinical value, and detailed roles of ZFPM2-AS1 in cervical cancer.
Methods: Reverse-transcription quantitative PCR was carried out to measure ZFPM2-AS1 expression in cervical cancer. A Cell Counting Kit-8 assay, flow cytometry, Transwell migration and invasion assays, and a tumor xenograft experiment were conducted to determine the influence of ZFPM2-AS1 on cervical cancer cell proliferation, apoptosis, migration, and invasion in vitro and on tumor growth in vivo, respectively.
Results: ZFPM2-AS1 was found to be aberrantly upregulated in cervical cancer, and its upregulation was associated with unfavorable values of clinical parameters. A ZFPM2-AS1 knockdown significantly reduced cervical cancer cell proliferation, migration, and invasion and increased apoptosis in vitro. The ZFPM2-AS1 knockdown decelerated tumor growth of cervical cancer cells in vivo. Molecular investigation indicated that ZFPM2-AS1 acts as a molecular sponge of microRNA-511-3p (miR-511-3p) in cervical cancer cells. Fibroblast growth factor receptor 2 (FGFR2) mRNA was validated as a direct target of miR-511-3p in cervical cancer, and its expression was positively modulated by ZFPM2-AS1. The effects of the ZFPM2-AS1 knockdown on malignant characteristics of cervical cancer cells were greatly attenuated by miR-511-3p inhibition.
Conclusion: ZFPM2-AS1 promotes cervical cancer progression through upregulation of miR-511-3p–FGFR2 axis output, thereby pointing to possible diagnostics and therapeutics based on the ZFPM2-AS1–miR-511-3p–FGFR2 pathway.

Keywords: ZFPM2 antisense RNA 1, cervical cancer therapy, fibroblast growth factor receptor 2, microRNA-511-3p

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