Long Non-Coding RNA SNHG7 Alleviates Oxygen and Glucose Deprivation/Reoxygenation-Induced Neuronal Injury by Modulating miR-9/SIRT1 Axis in PC12 Cells: Potential Role in Ischemic Stroke
Authors Zhou T, Wang S, Lu K, Yin C
Received 22 July 2020
Accepted for publication 19 October 2020
Published 24 November 2020 Volume 2020:16 Pages 2837—2848
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Jun Chen
Tao Zhou,1,* Shuai Wang,1,* Kai Lu,2 Chunhui Yin3
1Department of Neurosurgery, Zibo First Hospital, Zibo City 255200, People’s Republic of China; 2Department of Neurology, Liaocheng Third People’s Hospital, Liaocheng City 252000, People’s Republic of China; 3Department of Intervention Clinic, Weifang Hospital of Traditional Chinese Medicine, Weifang City 261000, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Chunhui Yin
Department of Intervention Clinic, Weifang Hospital of Traditional Chinese Medicine, No. 1055, Weizhou Road, Kuiwen District, Weifang City, Shandong Province 261000, People’s Republic of China
Tel +86- 0536-8190000
Email [email protected]
Objective: The roles of long non-coding RNA (lncRNAs) in ischemic stroke (IS) have been widely illustrated. Here, we focused on the function and mechanism of lncRNA SNHG7 in IS.
Methods: Middle cerebral artery occlusion (MCAO) was used for inducing mice to establish IS models in vivo. Oxygen and glucose deprivation/reoxygenation (OGD/R) was used for treating PC12 cells to establish IS models in vitro. Relative expression of SNHG7 and miR-9 was determined by qRT-PCR. The neuronal injury was assessed by measuring relative activity of ROS, malondialdehyde (MDA) level and cell viability. Cell viability was determined by MTT assay. Dual-luciferase reporter (DLR) assay was employed to test the target of SNHG7 or miR-9. Western blot was used to determine the protein expression of SIRT1. Apoptosis rate was measured by flow cytometry.
Results: SNHG7 was down-regulated and miR- 9 was up-regulated by MCAO treatment in brain tissues of mice and by OGD/R treatment in PC12 cells. Overexpression of SNHG7 or suppression of miR-9 decreased the relative activity of ROS and the MDA level as well as enhancing cell viability, and SNHG7 reduced apoptosis rate in OGD/R-induced PC12 cells (IS cells). MiR-9 was targeted by SNHG7 and SIRT1 was targeted by miR-9. The protein expression of SIRT1 was reduced by OGD/R treatment in PC12 cells. The suppressive effects of SNHG7 on the relative activity of ROS, the MDA level and apoptosis rate as well as the promotion effect of SNHG7 on cell viability were reversed by miR-9 mimics or sh-SIRT1 in IS cells.
Conclusion: LncRNA SNHG7 alleviated OGD/R-induced neuronal injury by mediating miR-9/SIRT1 axis in vitro.
Keywords: ischemic stroke, oxygen and glucose deprivation/reoxygenation, small nucleolar RNA host gene 7, miR-9, silent information regulator factor 2-related enzyme 1
Ischemic stroke (IS) is considered as a cerebrovascular disease with high disability and mortality, and it has high prevalence in the elderly.1 The major symptoms of IS include facial numbness, impair of memory, trouble speaking and partial paralysis.2,3 In the past few years, there is an increasing incidence rate of IS in people, which may result from multitudinous factors, such as hypercholesterolemia, hypertension, obesity and diabetes.4 Although traditional clinical treatments for IS5 including thrombolytic therapy, percutaneous intravascular interventions and medication have achieved effectiveness, the unsatisfactory result of IS therapy urges us seeking for new strategies to relieve IS.
Long non-coding RNAs (lncRNAs), an abundant component of the mammalian transcriptome, are a subtype of RNA with a length of more than 200 nucleotides and lack protein-coding ability.6 Previous studies have reported the potential roles of lncRNAs in IS.7 Chen et al have reported that silencing of lncRNA ROR can alleviate injury of PC12 cells in IS cell models induced by hypoxia/reoxygenation (H/R).8 Lv et al have revealed that lncRNA SNHG1 alleviates apoptosis and inflammation in IS cell model.9 Xiang et al have indicated that inhibition of lncRNA MEG3 markedly enhances cell viability and reduces cell apoptosis in IS cell model induced by oxygen-glucose deprivation and reoxygenation (OGD/R).10 Specifically, lncRNA small nucleolar RNA host gene 7 (SNHG7), located on chromosome 9q34.3, has been reported to make imperative impacts on diverse diseases.11–13 SNHG7 suppresses cell viability of cardiac fibroblasts in cardiac fibrosis.11 SNHG7 can inhibit the high glucose-induced proliferation and migration of human retinal endothelial cells in diabetic retinopathy (DR).12 SNHG7 can promote viability of cartilage cells and suppress apoptosis of cartilage cells in osteoarthritis.13 However, the role and mechanism of SNHG7 in IS have not been elucidated till now.
MicroRNAs (miRNAs), a class of small intracellular molecules with 18–23 nucleotides in length, can regulate gene expression at the post-transcriptional level.14 Many miRNAs are emerging as important molecular mediators of IS, such as miR-143,15 miR-27b16 and miR-191.17 As one of the most widely explored miRNAs, miR-9 has also been revealed to exert vital roles in IS.18–20 The suppression of miR-9 can reduce inflammation and neuronal apoptosis to alleviate neuronal injury in IS cell models induced by OGD/R.18 Overexpression of miR-9 promotes post-ischemic cell viability of the neuronal cells in IS cell models induced by OGD.19 Overexpression of miR-9 inhibits the apoptosis of neurons in IS cell models induced by OGD.20 Nonetheless, the regulatory effect of SNHG7 on miR-9 in IS has not been clarified.
Sirtuins (SIRTs) are a family of nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylases.21 According to the report, seven closely-related SIRT family members have been identified, including silent information regulator factor 2-related enzyme 1 (SIRT1), SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT7.22,23 Abundant studies have revealed the roles of lncRNAs and miRNAs in these sirtuin genes, such as the miR-140-5p/SIRT2 axis in myocardial oxidative stress,24 the miR-421/SIRT3 axis in H/R-induced oxidative stress,25 the lncRNA HMMR-AS1/miR-138/SIRT6 axis in lung adenocarcinoma26 and the miR-20b/SIRT7 axis in high glucose-induced podocyte apoptosis.27 SIRT1 is a NAD+-dependent deacetylase.28 Given that the expression of SIRT1 reduces with aging and SIRT1 slows down cellular senescence, researchers have focused on the involvement of SIRT1 in IS.29,30 Teertam et al have reported that miR-149-5p plays a regulatory role in neuronal cell death through SIRT1/p53 axis during IS.31 Rao et al have revealed that repression of miR-217 protects neurons against OGD/R-induced injury by targeting SIRT1.32 However, the regulatory mechanism between SIRT1 and miR-9 has not been explored in IS. In the current study, the expression and role of SNHG7 and miR-9 were probed in IS cell models. Furthermore, to further understand the molecular mechanism of SNHG7 in IS, the regulatory interrelation among SNHG7, miR-9 and SIRT1 was explored in IS cell models. Our finding might provide a novel target for the prevention and treatment of IS.
Materials and Methods
The Establishment of the Middle Cerebral Artery Occlusion (MCAO) Model
This study was performed after obtaining local ethical committee approval of Zibo First Hospital (No. 2,018,013), and all experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals. Male C57BL/6 mice (6–8 weeks) were bought from Shanghai Animal Laboratory Center (Shanghai, China). All mice were housed under conditions of constant temperature and humidity. Food and water were available ad libitum. Mice were divided into two groups (the sham group and the MCAO group), with five mice in each group. Mice in the MCAO group were anesthetized with 1.5–3% isoflurane. A 6–0 surgical nylon filament, coated with 1% poly-L-lysine and blunted at the tip, was inserted 10 mm into the internal carotid to occlude the origin of the middle cerebral artery (MCA) in mice. Cerebral blood flow (CBF) was determined in the area of MCA by laser Doppler flowmetry (Moor Instruments, Devon, UK). Only mice with >80% decrease of CBF in the MCA territory were included in the present study. After MCA obstruction for 1 h, the sutures were removed to recover blood flow and mice were allowed to recover for 24 h. In the sham group, all procedures were identical except for inserting an intraluminal filament. Finally, mice were anesthetized with pentobarbital sodium (50 mg/kg) and sacrificed by cervical dislocation. Brain tissues of mice were collected for following experiments.
Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
Total RNAs were extracted from PC12 cells or brain tissues of mice by using TRIzol® reagent (Takara, Dalian, China) and converted to complementary DNAs (cDNAs) with a PrimeScript™ RT reagent kit with gDNA Eraser (Takara). The qRT-PCR was performed by using SYBR Premix Ex Taq II (Takara) and the reaction procedure was shown as follows: initial denaturation at 95°C for 30 s, 40 cycles of 95°C for 10 s, 72°C for 45 s and 60°C for 15 s. The primers used in the current study were shown in Table 1. Finally, the relative expression of SNHG7 and miR-9 was calculated by the 2−ΔΔCt method, and GAPDH was chosen as an internal reference.
Table 1 Primers for Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) in Current Study
A rat adrenal pheochromocytoma cell line PC12 with specific characteristic of neural cells was used in accordance with previous studies.33–36 PC12 cells were bought from American Type Culture Collection (ATCC, USA). PC12 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin and incubated at 37°C under a humidified atmosphere with 5% CO2.
Establishment of OGD/R Model
The OGD/R model in PC12 cells was established as previously reported.37 Briefly, PC12 cells cultured in glucose-free DMEM medium were incubated at 37°C in a hypoxia condition with 5% CO2 and 95% N2. After hypoxia treatment for 4 h, PC12 cells were transferred back to normal DMEM medium containing 10% FBS and 4.5 g/L glucose, and cells were incubated at 37°C under an atmosphere of 95% air and 5% CO2 to recover. Then PC12 cells were harvested after recovery for 4 h, 8 h, 12 h and 24 h for following experiments. PC12 cells in the sham group did not receive the treatment of OGD/R.
The pcDNA3.1 (His tag in-frame with the 3ʹ-end of the cDNA; CMV promoter; neomycin resistance), pcDNA-SNHG7 (containing full-length rat SNHG7), short hairpin (sh)-negative control (NC), sh-SNHG7, miR-NC, miR-9 mimics, mimics NC, miR-9 inhibitor, inhibitor NC and sh-SIRT1 were bought from RiboBio Company (Beijing, China). Additionally, we have listed the sequences of shRNAs or miRs as follows: sh-SNHG7 (5ʹ-GCCTGGGTGTTGCTGTGTATT-3ʹ), sh-SIRT1 (5ʹ-CCATTCTTCAAGTTTGCAA-3ʹ), miR-9 mimics (5ʹ-UCAUACAGCUAGAUAACCAAAGA-3ʹ), mimics NC (5ʹ-UCACAGUGAACCGGUCUCUUU-3ʹ), miR-9 inhibitor (5ʹ-GCTAGATAACCAAAG-3ʹ) and inhibitor NC (5ʹ-ACGTCTATACGCCA-3ʹ). When cells grew 80–90% confluence, the above transcripts were transfected into PC12 cells with Lipofectamine3000 (Invitrogen, Carlsbad, CA, USA) for 48 h.
Detection of Reactive Oxygen Species (ROS) and Malondialdehyde (MDA)
A lipid peroxidation product MDA assay kit (ab118970, Abcam, MA, USA) was used for measuring the MDA level under the guidance of the manufacturer’s instructions. A cellular ROS assay kit (ab113851, Abcam) was employed to determine relative activity of ROS.
3-(4, 5-Dimethyl-2-Thiazolyl)-2, 5-Diphenyl-2-H-Tetrazolium Bromide (MTT) Assay
PC12 cells were plated in 96-well plates at a density of 5 × 103/well and then incubated with 20 µL MTT solution (Beyotime, Shanghai, China). After incubation for 4 h, dimethyl sulfoxide (DMSO; 150 µL) was added to dissolve the remaining formazan crystals. Cell viability was evaluated by measuring the optical density (OD) at 450 nm with a microplate reader (BMG LABTECH, Durham, NC, USA).
Dual-Luciferase Reporter (DLR) Assay
The 3ʹ-UTR fragment of wild type (WT) SNHG7 or WT SIRT1 containing the complementary sequence of miR-9 was synthesized and introduced into a pGL3 Basic Vector (Promega, Madison, WI) to generate SNHG7 WT vector or SIRT1 WT vector. In a similar way, the 3ʹ-UTR fragment of mutant type (mut) SNHG7 or mut SIRT1 including the mutant sequence of miR-9 was synthesized and inserted into a pGL3 Basic Vector (Promega) to form SNHG7 mut vector or SIRT1 mut vector. Then PC12 cells were co-transfected with a WT vector/mut vector and miR-NC/miR-9 mimics for 48 h. Then relative luciferase activity was measured by Dual-Luciferase Reporter Assay System (Promega).
Total proteins were extracted from PC12 cells and brain tissues of mice by RIPA buffer (Beyotime). Equal proteins of each sample were separated by 10% sodium dodecyl sulphate-polyacrylamide gels (SDS-PAGE). Next, separated proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, Billerica, MA, USA) and blocked with 5% non-fat milk for 1 h. Then protein samples were incubated with the following primary antibodies overnight at 4°C: anti-SIRT1 (1:1000, ab189494, Abcam) and anti-β-actin (1:6000, ab115777, Abcam). After the membranes were washed with tris buffered saline Tween (TBST), the horseradish-conjugated secondary antibody (1:10,000, ab205718, Abcam) was added to incubate at 37°C for 2 h. The immune-reactivity was visualized with a Chemiluminescence Detection Kit (Thermo Fisher Scientific, Shanghai, China) and the relative protein expression of SIRT1 over the β-actin was quantified by Gel-pro Analyzer software (Media Cybernetics, Maryland, USA).
Apoptosis rate was analyzed by an Annexin V-FITC/PI kit (Beyotime) on flow cytometry. Apoptotic cells (PC12 cells) were labeled with FITC-AnnexinV and PI for 30 min in the dark. The fluorescence was determined on cytoFLEX LX flow cytometer (Beckman-Counter Electronics, Jiangsu, China) using CytExpert software. Quadrants were positioned on Annexin V/PI plots to distinguish apoptotic cells
The targets of SNHG7 were predicted by starbase (http://starbase.sysu.edu.cn/agoClipRNA.php?), and a total of 82 targets for SNHG7 were predicted. Among these targets, miR-9 plays an important role in IS, and the regulatory effect of SNHG7 on miR-9 in IS has not been investigated. Thus, we chose miR-9 as a target in this study.
The targets of miR-9 were predicted by starbase (http://starbase.sysu.edu.cn/agoClipRNA.php?), and a total of 5, 413 targets for miR-9 were predicted. Among these targets, SIRT1 is important in IS, and the regulatory mechanism between miR-9 and SIRT1 has not been explored in IS. Additionally, we have verified the targeting relationship between SIRT1 and miR-9 by miRDB. Therefore, we chose SIRT1 as a target in this study.
Experiments of the present study were implemented in triplicate. The SPSS Statistics 22.0 was utilized for the statistical analysis of data. All data were shown as the mean ± standard deviation. Student’s t-test was employed to assess the differences between two groups. The One-way ANOVA method was used for multiple comparisons, followed by a Tukey’s multiple comparisons test for pairwise comparisons. The P value less than 0.05 was regarded as statistically significant.
SNHG7 Was Down-Regulated in Brain Tissues of MCAO-Induced Mice and OGD/R-Induced PC12 Cells, and It Mitigated OGD/R-Induced Neuronal Injury in PC12 Cells
In order to explore the function of SNHG7 in IS, we firstly determined the relative expression of SNHG7 in brain tissues of MCAO mice and OGD/R-induced PC12 cells by qRT-PCR. The result showed that SNHG7 was markedly decreased in brain tissues of MCAO mice compared with brain tissues of mice without MCAO treatment (P < 0.001, Figure 1A). SNHG7 was also remarkably decreased by OGD/R treatment at 4 h, 8 h and 12 h in PC12 cells (P < 0.001, Figure 1B). Then we silenced and overexpressed SNHG7. As shown in Figure 1C, SNHG7 was up-regulated by transfection of pcDNA-SNHG7 and down-regulated by transfection of sh-SNHG7 in PC12 cells (all P < 0.001). The results of functional assays demonstrated that the relative activity of ROS and the MDA level of PC12 cells in the OGD/R group were increased compared with the NC group, while cell viability of PC12 cells in the OGD/R group was notably decreased in comparison with the NC group (all P < 0.001, Figure 1D–F). Of note, the relative activity of ROS and the MDA level were reduced by overexpression of SNHG7 in OGD/R-induced PC12 cells, and cell viability was enhanced by overexpression of SNHG7 in OGD/R-induced PC12 cells (all P < 0.001, Figure 1D–F). In comparison to overexpression of SNHG7, knockdown of SNHG7 had opposite effects on cell viability, relative activity of ROS and the MDA level in OGD/R-induced PC12 cells (all P < 0.001, Figure 1D–F).
SNHG7 Could Serve as a Competing Endogenous RNA (ceRNA) for miR-9
To further explore the mechanism of SNHG7 in IS, we predicted the target genes of SNHG7 by starbase2.0. We observed that SNHG7 had potential binding sites for miR-9 (Figure 2A). Then the interaction between SNHG7 and miR-9 was tested by DLR assay. The result showed that relative luciferase activity of PC12 cells was significantly reduced by co-transfection of SNHG7 WT vector and miR-9 mimics (P < 0.001, Figure 2B), whereas relative luciferase activity of PC12 cells showed no statistical change after co-transfection of SNHG7 mut vector and miR-9 mimics (Figure 2B). Relative expression of miR-9 was increased in brain tissues of MCAO mice in relative to that in brain tissues of mice without MCAO treatment (P < 0.001, Figure 2C). The result of Pearson’s correlation analysis showed a negative correlation between SNHG7 and miR-9 in brain tissues of MCAO mice (P < 0.0001, Figure 2D). In addition, relative expression of miR-9 was elevated by OGD/R treatment at 4 h, 8 h, 12 h and 24 h in PC12 cells (P < 0.001, Figure 2E).
Inhibition of miR-9 Alleviated OGD/R-Induced Neuronal Injury in PC12 Cells
Afterwards, we explored the expression and function of miR-9 in PC12 cells. We observed that the expression of miR-9 was obviously elevated by OGD/R treatment in PC12 cells (P < 0.001, Figure 3A). MiR-9 was evidently increased by transfection of miR-9 mimics and decreased by transfection of miR-9 inhibitor in OGD/R-induced PC12 cells (all P < 0.001, Figure 3A). The relative activity of ROS and the MDA level of PC12 cells in the OGD/R group were increased in comparison with the NC group, whereas the viability of PC12 cells in the OGD/R group was significantly decreased in comparison with the NC group (all P < 0.001, Figure 3B–D). Importantly, the relative activity of ROS and the MDA level were reduced by inhibition of miR-9 in OGD/R-induced PC12 cells, while cell viability was enhanced by inhibition of miR-9 in OGD/R-induced PC12 cells (all P < 0.001, Figure 3B–D). In contrast to inhibition of miR-9, up-regulation of miR-9 had opposite effects on cell viability, the relative activity of ROS and the MDA level in OGD/R-induced PC12 cells (all P < 0.001, Figure 3B–D).
SIRT1 Was Targeted by miR-9
Subsequently, we predicted the target genes of miR-9 with starbase2.0. It was observed that SIRT1 had a conserved binding region of miR-9 (Figure 4A). The result of DLR revealed that relative luciferase activity of PC12 cells was reduced by co-transfection of SIRT1 WT vector and miR-9 mimics (P < 0.001, Figure 4B), whereas relative luciferase activity of PC12 cells showed no significant change after co-transfection of SIRT1 mut vector and miR-9 mimics (Figure 4B). The result of Western blot showed that SIRT1 was down-regulated in brain tissues of MCAO mice in relative to that in brain tissues of mice without MCAO treatment (P < 0.001, Figure 4C). There was a negative correlation between SIRT1 and miR-9 in brain tissues of MCAO mice (P < 0.0001, Figure 4D). Furthermore, SIRT1 was decreased by OGD/R treatment at 4 h, 8 h, 12 h and 24 h in PC12 cells (P < 0.001, Figure 4E).
LncRNA SNHG7 Mitigated OGD/R-Induced Neuronal Injury via Regulating miR-9/SIRT1 Axis in PC12 Cells
Based on above results, we hypothesized that SNHG7 might repress the injury of PC12 cells through mediating miR-9/SIRT1 axis. To verify this, we firstly explored the interrelation among SNHG7, miR-9 and SIRT1. The result of Western blot demonstrated that SIRT1 was up-regulated by overexpression of SNHG7, and the promotion effect of SNHG7 on SIRT1 was reversed by miR-9 mimics or sh-SIRT1 in OGD/R-induced PC12 cells (all P < 0.001, Figure 5A). Then rescue assays were performed. The results indicated that cell viability was promoted by overexpression of SNHG7, and the promotion effect of SNHG7 on cell viability was reversed by overexpression of miR-9 or knockdown of SIRT1 in OGD/R-induced PC12 cells (all P < 0.001, Figure 5B). The relative activity of ROS and the MDA level were reduced by overexpression of SNHG7, and the suppression effects of SNHG7 on the relative activity of ROS and the MDA level were reversed by overexpression of miR-9 or knockdown of SIRT1 in PC12 cells induced by OGD/R (all P < 0.001, Figure 5C and D). Moreover, apoptosis rate of OGD/R-induced PC12 cells was reduced by up-regulation of SNHG7, while the reduction effect of SNHG7 on apoptosis rate was reversed by up-regulation of miR-9 or knockdown of SIRT1 in OGD/R-induced PC12 cells (all P < 0.001, Figure 5E).
IS is a severe neurological disease with serious impairment of the neurological function.38 Growing literature has shown that down-regulation of lncRNAs is implicated in the pathological process of IS, such as lncRNA Oprm1,39 lncRNA ZFAS140 and lncRNA ANRIL.34 Coincident with expression trend of previous studies, we also observed that SNHG7 was down-regulated by MCAO treatment in brain tissues of mice and by OGD/R treatment in PC12 cells, implying that SNHG7 might involve in the pathologies of IS. Besides, numerous studies have revealed the key role of SNHG7 in many diseases.11–13 SNHG7 can inhibit the high glucose-induced proliferation and migration of human retinal endothelial cells in DR.12 SNHG7 promotes cell viability and represses cell apoptosis in osteoarthritis.13 SNHG7 suppresses cell viability of cardiac fibroblasts in cardiac fibrosis.11 In the present study, we found that the relative activity of ROS, apoptosis rate and the MDA level were reduced by SNHG7, while the viability was stimulated by SNHG7 in PC12 cells induced by OGD/R, suggesting that SNHG7 mitigated OGD/R-induced neuronal injury in PC12 cells. Based on these outcomes, we inferred that SNHG7 might be a promising target for IS therapy.
In recent years, the expression and function of miR-9 in IS have drawn wide attention.18,19,41 Xue et al have revealed that miR-9 is up-regulated by OGD treatment in neurons, and the suppression of miR-9 remarkably alleviates neuronal injury in IS cell models by decreasing OGD-induced neuronal apoptosis.18 Ji et al have revealed that the level of serum miR-9 is markedly higher in acute IS patients than that in non-stroke volunteers.41 Nampoothiri et al have reported that inhibition of miR-9 enhances viability of neuronal cells in IS cell models.19 Consistently, we also observed that miR-9 was increased by MCAO treatment in brain tissues of mice and by OGD/R induction in PC12 cells. Moreover, we observed that down-regulation of miR-9 reduced the relative activity of ROS and the MDA level in PC12 cells induced by OGD/R, while promoted viability of PC12 cells induced by OGD/R. These results of functional assays revealed that inhibition of miR-9 alleviated neuronal injury in IS cell models induced by OGD/R, and the role of miR-9 in our study was similar to previous studies. Besides, miR-9 has been revealed to interact with SNHG7 in skeletal fracture42 and in malignant melanoma.43 Similarly, we also found that miR-9 was targeted by SNHG7 and negatively correlated with SNHG7. Meanwhile, we discovered that overexpression of miR-9 reversed the suppression effects of SNHG7 on the relative activity of ROS and the MDA level as well as the promotion effect of SNHG7 on cell viability in OGD/R-induced PC12 cells. Collectively, SNHG7 might alleviate neuronal injury by sponging miR-9 in IS cell models.
Previously, many studies have reported the expression and role of SIRT1 in IS.29,30 Yan et al have revealed that SIRT1 is down-regulated in the stroke group compared with the normal group, and rosuvastatin ameliorates cerebral infarction condition of rats with cerebral IS by SIRT1/NF-κB pathway.44 Teertam et al have indicated that the expression of SIRT1 is down-regulated in the MCAO group in comparison with the sham group, and overexpression of SIRT1 decreases the loss of pyramidal neuron in brains of IS mice.31 In line with previous literature, we found that SIRT1 was down-regulated by MCAO treatment in brain tissues of mice and by OGD/R treatment in PC12 cells, suggesting that SIRT1 might alleviate IS. Furthermore, it has been reported that SIRT1 can act as the downstream target of miR-199a in cerebral ischemia45 and miR-149-5p in acute IS.31 Here, we observed that SIRT1 was targeted by miR-9 and negatively correlated with miR-9. Based on above results, we speculated that inhibition of miR-9 alleviated neuronal injury by targeting SIRT1 in IS cell models. Meantime, we discovered that the knockdown of SIRT1 reversed the inhibition effects of SNHG7 on the relative activity of ROS, the MDA level and apoptosis rate as well as the promotion effect of SNHG7 on cell viability in OGD/R-induced PC12 cells. At length, we deduced that SNHG7 might attenuate OGD/R-induced neuronal injury by mediating miR-9/SIRT1 axis in vitro.
To sum up, SNHG7 was strikingly down-regulated by MCAO treatment in brain tissues of mice and by OGD/R treatment in PC12 cells. DLR assay verified that SNHG7 could act as the sponge of miR-9 and SIRT1 was the downstream target of miR-9 in brain tissues of mice. Overall, our results indicated that SNHG7 mitigated neuronal injury through competition with miR-9 to regulate the expression of SIRT1 in IS cell models induced by OGD/R. SNHG7 was a promising therapeutic target for attenuating IS. However, the functional experiments of SNHG7 in vivo were not performed in our study, and further study is needed to verify our findings.
Long non-coding RNA, lncRNAs; ischemic stroke, IS; Middle cerebral artery occlusion, MCAO; Oxygen and glucose deprivation/reoxygenation, OGD/R; Malondialdehyde, MDA; Dual-luciferase reporter, DLR.
This study was conducted after obtaining local ethical committee approval of Zibo First Hospital (No. 2018013) and We confirm that all experiments were executed in accordance with the Guide for the Care and Use of Laboratory Animals.
There is no funding to report.
The authors report no conflicts of interest for this work.
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