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Long Non-Coding RNA HCP5 Facilitates Cell Invasion And Epithelial-Mesenchymal Transition In Oral Squamous Cell Carcinoma By miR-140-5p/SOX4 Axis

Authors Zhao J, Bai X, Feng C, Shang X, Xi Y

Received 9 September 2019

Accepted for publication 25 October 2019

Published 12 December 2019 Volume 2019:11 Pages 10455—10462

DOI https://doi.org/10.2147/CMAR.S230324

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Antonella D'Anneo


Jianling Zhao,1,* Xijing Bai,2,* Chong Feng,3 Xinhua Shang,4 Yuli Xi5

1Experimental Center of Dental Hospital Affiliated to Jiamusi University, Jiamusi City 154002, Heilongjiang Province, People’s Republic of China; 2Department of Oral and Maxillofacial Surgery, Tianjin Stomatology Hospital, Tianjin City 300041, People’s Republic of China; 3Department of Orthodontics, Tianjin Stomatology Hospital, Tianjin City 300041, People’s Republic of China; 4Binhu Outpatient Department, Hefei Stomatological Hospital, Hefei City 230001, Anhui Province, People’s Republic of China; 5Department of Stomatology, Hongqi Hospital Affiliated to Mudanjiang Medical University, Mudanjiang City 157000, Heilongjiang Province, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Yuli Xi
Department of Stomatology, Hongqi Hospital Affiliated to Mudanjiang Medical University, No. 1 Taiping Road, Xi’an District, Mudanjiang City 157000, Heilongjiang Province, People’s Republic of China
Email xyl13674539662@163.com

Background: Oral squamous cell carcinoma (OSCC) is the predominant histological type of human oral cancer. In this study, we sought to investigate the functional role of lncRNA HCP5 in OSCC progression.
Methods: The HCP5 and miR-140-5p expression level was determined in 73 paired OSCC tissues and their adjacent normal tissues. Knockdown or overexpression of HCP5 was conducted to investigate the effects of HCP5 on malignant behaviors of OSCC cells. Then, bioinformatic prediction and dual-luciferase reporter assay were conducted to study the interaction between HCP5 and miR-140-5p in OSCC.
Results: Our results demonstrated that HCP5 expression was significantly increased in OSCC tissues and cell lines. High HCP5 level was associated with the aggressive clinicopathological characteristics and poor prognosis of OSCC patients. In vitro gain- and loss-of-function experiments showed that HCP5 overexpression promoted, whereas HCP5 knockdown inhibited the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of OSCC cells. Mechanistically, we confirmed that HCP5 might serve as a competitive endogenous RNA (ceRNA) for miR-140-5p to alleviate the repression of its downstream target, SOX4, a master regulator of EMT. Furthermore, restoration of miR-140-5p expression diminished the oncogenic effects of HCP5 on OSCC cells.
Conclusion: Overall, the present study indicated that HCP5/miR-140-5p/SOX4 axis might be a ponderable and promising therapeutic target for OSCC.

Keywords: oral squamous cell carcinoma, long non-coding RNA HCP5, epithelial-mesenchymal transition, competitive endogenous RNA

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