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Long Non-Coding RNA BCAR4 Binds to miR-644a and Targets TLX1 to Promote the Progression of Bladder Cancer

Authors Wang X, He H, Rui W, Xie X, Wang D, Zhu Y

Received 30 September 2019

Accepted for publication 25 February 2020

Published 24 March 2020 Volume 2020:13 Pages 2483—2490

DOI https://doi.org/10.2147/OTT.S232965

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Geoffrey Pietersz


Xiaojing Wang,* Hongchao He,* Wenbin Rui, Xin Xie, Dawei Wang, Yu Zhu

Department of Urology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Dawei Wang; Yu Zhu
Department of Urology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, People’s Republic of China
Email wdwrjhn@163.com; yu_zhu111@sina.com

Background: Bladder cancer is a serious threat to human health. It is meaningful to study the pathogenesis of bladder cancer. Long non-coding RNAs (lncRNAs) are reported to promote or inhibit bladder cancer development. However, the role of lncRNA BCAR4 in the regulation of bladder cancer remains unclear.
Purpose: This study was to explore the role of lncRNA BCAR4 in the progression of bladder cancer cell.
Methods: RT-PCR was used to examine the expression of BCAR4 and miR-644a. CCK8 assay, colony formation assay, Transwell assay were used to detect the progression of bladder cancer cells after transfecting of indicated plasmids.
Results: The expression of BCAR4 was higher in bladder cancer cell lines than normal urothelial cell line. Moreover, the expression of BCAR4 was associated with the advanced stage and metastasis of bladder cancer. Through knockdown of BCAR4, we discovered that knockdown of BCAR4 significantly decreased the proliferation, migration and invasive abilities of bladder cancer cells. Mechanically, we showed that BCAR4 can bind to miR-644a directly and targets TLX1. Moreover, we also showed that miR-644a was also highly expressed in bladder cancer cells and inhibition of miR-644a or overexpression of TLX1 can increased the migration abilities of bladder cancer caused by knockdown of BCAR4.
Conclusion: We showed that BCAR4 sponged miR-644a to modulate the expression of TLX1 and promote bladder cancer development.

Keywords: BCAR4, miR-644a, TLX1, bladder cancer, proliferation and migration

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