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LncRNA TDRG1 Promotes Proliferation, Invasion and Epithelial-Mesenchymal Transformation of Osteosarcoma Through PI3K/AKT Signal Pathway

Authors Huang Y, Xu YQ, Feng SY, Zhang X, Ni JD

Received 9 February 2020

Accepted for publication 23 April 2020

Published 15 June 2020 Volume 2020:12 Pages 4531—4540

DOI https://doi.org/10.2147/CMAR.S248964

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Eileen O'Reilly


Yan Huang,1 Yong-Qiang Xu,2 Si-Yin Feng,2 Xiang Zhang,2 Jiang-Dong Ni1

1Department of Orthopaedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan Province, People’s Republic of China; 2Department of Orthopaedics, Hunan Provincial People’s Hospital, Changsha, Hunan Province, People’s Republic of China

Correspondence: Jiang-Dong Ni
Department of Orthopaedics, The Second Xiangya Hospital of Central South University, No. 139, Renmin Middle Road, Furong District, Changsha, Hunan Province, People’s Republic of China
Tel +86-73185295999
Email nijiangdong001@csu.edu.cn

Objective: This study aimed to investigate the effect of long non-coding TDRG1 on proliferation and migration of osteosarcoma cells through PI3K/AKT signaling pathway.
Materials and Methods: Altogether 87 cases of osteosarcoma tissues and adjacent tissues were collected, and osteosarcoma cells and osteoblasts were purchased. The expression of LncRNA TDRG1 in tissues and cells was detected by RT-PCR. Si-NC, si-TDRG1, and Sh-TDRG1 were transfected into osteosarcoma cells. L740Y-P (activator of PI3K/AKT pathway) and LY294002 (inhibitor of PI3k/AKT pathway) were used to interfere with PI3k/Akt signaling pathway in osteosarcoma cells. qRT-PCR was used to detect the expression of TDRG1 in osteosarcoma tissues and cells. WB was used to detect the expression of p-PI3K, p-AKT, N-cadherin, E-Cadherin, vimentin, Bax, Caspase-3, and Bcl-2 in cells. CCK-8, Transwell and cell scratch tests were used to detect cell proliferation, invasion and migration, and flow cytometry was used to detect cell apoptosis.
Results: TDRG1 was highly expressed in osteosarcoma, and the levels of p-PI3K and p-AKT were also up-regulated. Cell experiments showed that inhibiting the expression of TDRG1 could inhibit the proliferation, invasion, migration and EMT of osteosarcoma cells, promote the apoptosis of cells, and up-regulating the expression of TDRG1 could promote the proliferation, invasion, migration and EMT of osteosarcoma cells and inhibit the apoptosis of cells. The 740Y-P intervention could reverse the inhibition of Si-TDRG1 on osteosarcoma cell proliferation, invasion, migration and EMT and the promotion of cell apoptosis. LY294002 intervention could reverse the promotion of Sh-TDRG1 on osteosarcoma cell proliferation, invasion, migration and EMT and the inhibition of cell apoptosis.
Conclusion: TDRG1 is highly expressed in osteosarcoma tissue. Silencing the expression of osteosarcoma can inhibit the proliferation, invasion, migration and EMT of osteosarcoma cells by inhibiting PI3K/AKT signaling pathway, which may be a new target for diagnosis and treatment of osteosarcoma.

Keywords: LncRNA TDRG1, PI3K/AKT, osteosarcoma cells, proliferation, migration

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