LncRNA NR2F2-AS1 Silencing Induces Cell Cycle Arrest in G0/G1 Phase via Downregulating Cyclin D1 in Colorectal Cancer
Authors Liu J, Qian J, Mo Q, Tang L, Xu Q
Received 4 July 2019
Accepted for publication 27 January 2020
Published 11 March 2020 Volume 2020:12 Pages 1835—1843
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Dr Seema Singh
Jianfeng Liu, Jun Qian, Qi Mo, Liming Tang, Qiang Xu
Department of Gastrointestinal Surgery, Changzhou No.2 People’s Hospital of Nanjing Medical University, Changzhou City, Jiangsu Province 213003, People’s Republic of China
Correspondence: Qiang Xu
Department of Gastrointestinal Surgery, Changzhou No.2 People’s Hospital of Nanjing Medical University, No. 29 Xinglong Road, Changzhou City, Jiangsu Province 213003, People’s Republic of China
Purpose: LncRNA NR2F2-AS1 has been characterized as an oncogenic lncRNA in non-small cell lung cancer. This study aims to explore the role of NR2F2-AS1 in colorectal cancer (CRC).
Methods: All CRC patients (n=60, 35 males and 25 females, 40 to 68 years old) in this study were enrolled in Changzhou No.2 People’s Hospital of Nanjing Medical University during the time period between July 2011 and December 2013. Tumor samples, CRC cells, vectors, transient transfections, RT-qPCR, western-blotting, as well as measurements of cell cycle, apoptosis and cell proliferation assay were carried out during the research.
Results: Our data showed that NR2F2-AS1 was upregulated in CRC and CRC patients with high levels of NR2F2-AS1 showed a low overall survival rate. Cyclin D1 was also upregulated in CRC and a positive correlation was found between Cyclin D1 and NR2F2-AS1. In CRC cells, NR2F2-AS1 siRNA silencing mediated the downregulation of Cyclin D1 and G0/G1 arrest, while Cyclin D1 overexpression rescued G0/G1 arrest caused by NR2F2-AS1 siRNA silencing.
Conclusion: Our results indicated that NR2F2-AS1 silencing mediates the downregulation of Cyclin D1 to induce G0/G1 arrest in colorectal cancer.
Keywords: colorectal cancer, lncRNA NR2F2-AS1, Cyclin D1, G0/G1 arrest
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