lncRNA NEAT1 Facilitates Cell Proliferation, Invasion and Migration by Regulating CBX7 and RTCB in Breast Cancer
Authors Yan L, Zhang Z, Yin X, Li Y
Received 2 December 2019
Accepted for publication 25 February 2020
Published 24 March 2020 Volume 2020:13 Pages 2449—2458
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Prof. Dr. Geoffrey Pietersz
This paper has been retracted.
Lixia Yan, 1,* Ze Zhang, 1,* Xingmei Yin, 1 Yongxia Li 2
1Department of Breast and Thyroid Surgery, Dongying People’s Hospital, Dongying, Shandong 257091, People’s Republic of China; 2Department of Stomatology and Eye, Dongying People’s Hospital, Dongying, Shandong 257091, People’s Republic of China
*These authors contributed equally to this work
Correspondence: Xingmei Yin
Department of Breast and Thyroid Surgery, Dongying People’s Hospital, Dongying People’s Hospital, No. 317, Nanyi Road, Dongying, Shandong 257091, People’s Republic of China
Purpose: To investigate the association between the lncRNA NEAT1 and breast cancer, and to determine the influence of NEAT1 on regulation of other signaling molecules in breast cancer.
Methods: In the present study, we measured levels of the lncRNA NEAT1 in 106 breast cancer patients and in a human breast cancer cell line by qRT-PCR. The correlation between NEAT1 expression and patients’ clinical characteristics was analyzed with in-house and TCGA data. We used cellular functioning assays and cell immunofluorescence assay to evaluate the role of NEAT1 and its target molecules in proliferation, invasion and migration in breast cancer. We used Western blotting to explore possible targets of NEAT1 and a subcellular fractionation assay to locate NEAT1 expression.
Results: NEAT1 was overexpressed in breast cancer tissue and also closely related to advanced clinical stages and positive lymph node metastases. NEAT1 levels were also tightly correlated to prognosis for breast cancer patients in survival analyses. Cellular function assays revealed that downregulation of NEAT1 could inhibit breast cancer cell viability, invasion and migration. Western blotting revealed down-regulation of CBX7 and up-regulation of RTCB following NEAT1 inhibition. Based on the cytoplasmic and nuclear expression of NEAT1, we investigated the possible regulation of CBX7 and RTCB by NEAT1. Results showed that NEAT1 regulated the expression of CBX7 and RTCB, possibly by binding of NEAT1 to DNA in the nucleus, which facilitates cell proliferation, invasion and migration.
Conclusion: The current results suggest that the lncRNA NEAT1 is upregulated in breast cancer and facilitates tumor cell viability, invasion and migration via CBX7 and RTCB.
Keywords: breast cancer, clinical biomarkers, NEAT1, CBX7, RTCB
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