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lncRNA MT1JP Suppresses Biological Activities of Breast Cancer Cells in vitro and in vivo by Regulating the miRNA-214/RUNX3 Axis

Authors Ouyang Q, Cui Y, Yang S, Wei W, Zhang M, Zeng J, Qu F

Received 8 December 2019

Accepted for publication 1 April 2020

Published 4 June 2020 Volume 2020:13 Pages 5033—5046

DOI https://doi.org/10.2147/OTT.S241503

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Yong Teng


Qianwen Ouyang,1 Yanru Cui,2 Shixin Yang,1 Wensong Wei,1 Mingyue Zhang,3 Jie Zeng,1 Fei Qu3

1Department of Breast Surgery, The Third Hospital of Nanchang, Jiangxi, China Jiangxi Province Key Laboratory for Breast Diseases, Nanchang, Jiangxi 330009, People’s Republic of China; 2Department of Physiology, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, People’s Republic of China; 3Department of Pharmacology, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, People’s Republic of China

Correspondence: Fei Qu
Department of Pharmacology, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, People’s Republic of China
Email qufei1107@sina.com

Introduction: The purpose of our research was to evaluate MT1JP in breast cancer.
Material and Methods: For clinical purpose, tissues were collected, and a correlation analysis ofMT1JP and miRNA-214 gene expressions was conducted. Using an in vitro study, MDA-MB-231 and MCF-7 cell lines were used as research objects in our research. Colony, flow cytometry, TUNEL, transwell, adhesion and wound healing assay were used to discuss the biological activities of the cells. In an in vivo study, tumor weight and volume were measured, and cell apoptosis was measured by TUNEL assay. The relative mechanism’s proteins were evaluated by Western blotting or immunohistochemistry assay.
Results: Compared with adjacent tissues, MT1JP and miRNA-214 gene expressions were significantly different (P< 0.001, respectively). By in vitro and in vivo studies, the biological activities of the cells were significantly decreased in MDA-MB-231 and MCF-7 cell lines with MT1JP overexpression. The relative mechanism was correlated with miRNA-214/RUNX3 axis.
Conclusion: The overexpression of MT1JP suppresses the biological activities of breast cancer cells by regulation miRNA-214/RUNX3 axis in vitro and vivo study.

Keywords: MT1JP, MCF-7, MDA-MB-231, invasion, proliferation, migration

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