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lncRNA MEG8 Upregulates miR-770-5p Through Methylation and Promotes Cell Apoptosis in Diabetic Nephropathy

Authors Zhang J, Song L, Ma Y, Yin Y, Liu X, Luo X, Sun J, Wang L

Received 24 March 2020

Accepted for publication 30 May 2020

Published 10 July 2020 Volume 2020:13 Pages 2477—2483

DOI https://doi.org/10.2147/DMSO.S255183

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Professor Ming-Hui Zou


Jinmei Zhang,1 Liwen Song,2 Yanjuan Ma,3 Yan Yin,1 Xinqi Liu,1 Xinyu Luo,1 Jiali Sun,1 Liqin Wang1

1Department of Endocrinology, Weifang Hospital of Traditional Chinese Medicine, Weifang, Shandong Province 261000, People’s Republic of China; 2Department of Endocrinology, Weifang People’s Hospital, Weifang, Shandong Province 261000, People’s Republic of China; 3Department of Endocrinology, Sunshine Fusion Hospital, Sunshine, Shandong Province 261061, People’s Republic of China

Correspondence: Liwen Song
Department of Endocrinology, Weifang People’s Hospital, Weifang City, Shandong Province 261000, People’s Republic of China
Tel +86 536-963360
Email zjmmjmdr@126.com

Background: It has been reported that lncRNA MEG8 can be induced by glucose in mice model of kidney injury, indicating its role in diabetic nephropathy (DN). This study was carried out to explore the role of MEG8 in DN.
Materials and Methods: The expression of MEG8 and miR-770-5p in plasma samples from DN patients (n = 66), diabetic patients (DM patients with no complications, n = 66) and healthy controls (n = 66) was detected by RT-qPCR. The interaction between MEG8 and miR-770-5p in podocyte cells was evaluated by transient transfections. Cell apoptosis under high-glucose treatment was detected by cell apoptosis assay.
Results: MEG8 and miR-770-5p were upregulated in plasma of DM patients and were further upregulated in DN patients. MEG8 was positively correlated with miR-770-5p. In podocyte cells, high-glucose treatment resulted in increased expression levels of MEG8 and miR-770-5p. In podocyte cells, overexpression of MEG8 resulted in upregulated expression of miR-770-5p and decreased methylation of the miR-770-5p gene. Cell apoptosis analysis showed that overexpression of MEG8 and miR-770-5p resulted in increased cell apoptotic rate under glucose treatment. In addition, combined overexpression of MEG8 and miR-770-5p showed stronger effects.
Conclusion: MEG8 may upregulate miR-770-5p through methylation to promote DN by promoting cell apoptosis.

Keywords: diabetic nephropathy, MEG8, miR-770-5p, apoptosis

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