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LncRNA LEF1-AS1 Promotes Ovarian Cancer Development Through Interacting with miR-1285-3p

Authors Zhang Y, Ruan F

Received 17 August 2019

Accepted for publication 30 November 2019

Published 30 January 2020 Volume 2020:12 Pages 687—694

DOI https://doi.org/10.2147/CMAR.S227652

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Ahmet Emre Eskazan


Yanan Zhang, Fang Ruan

Department of Obstetrics and Gynecology, Affiliated Hospital of Jining Medical College, Jining 272000, People’s Republic of China

Correspondence: Fang Ruan
Department of Obstetrics and Gynecology, Affiliated Hospital of Jining Medical College, 89 Guhuai Road, Jining, Shandong 272000, People’s Republic of China
Email fang_ruan@163.com

Background: Growing evidence indicates that long noncoding RNA (lncRNA) is a group of important regulator in cancer development. However, the correlation between lncRNA and ovarian cancer remains elusive. Here, we aimed to investigate the roles of LEF1-AS1 in ovarian cancer progression.
Methods: LEF1-AS1 expression was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Survival rate was analyzed by Kaplan-Meier method. Cell Counting Kit-8 (CCK8) and colony formation assays were used for proliferation analysis. Transwell assay was utilized for analyses of migration and invasion. Luciferase reporter assay was performed to test the interaction between LEF1-AS1 and miR-1285-3p.
Results: We showed that LEF1-AS1 expression was upregulated in ovarian cancer tissues compared with normal tissues. Besides, LEF1-AS1 level was positively correlated with lymph node metastasis and advanced stage. Enhanced expression of LEF1-AS1 may predict a poor prognosis. Moreover, LEF1-AS1 knockdown suppressed ovarian cancer cell proliferation, migration and invasion. Mechanistically, LEF1-AS1 exerted its oncogenic functions through interacting with miR-1285-3p to inhibit miRNA activity. Rescue assay validated that miR-1285-3p inhibitors abrogated LEF1-AS1-silencer-caused suppression of ovarian cancer progression.
Conclusion: Our study revealed that LEF1-AS1 acts as a vital regulation in ovarian cancer progression.

Keywords: ovarian cancer, LEF1-AS1, miR-1285-3p, progression


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