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lncRNA HAND2-AS1 Regulates Prostate Cancer Cell Growth Through Targeting the miR-106a-5p/RBM24 Axis

Authors Wei P, Yang J, Zhang D, Cui M, Li L

Received 16 January 2020

Accepted for publication 7 April 2020

Published 21 May 2020 Volume 2020:13 Pages 4523—4531

DOI https://doi.org/10.2147/OTT.S246274

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Carlos E Vigil


Pengtao Wei,1 Jing Yang,2 Dandan Zhang,3 Meng Cui,4 Lianjun Li5,6

1Department of Urology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang 471000, People’s Republic of China; 2Central Sterile Supply Department, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, People’s Republic of China; 3Urinary Surgery, YiDu Central Hospital in Weifang City, Qingzhou, People’s Republic of China; 4Department of Gynecology, Shandong Provincial Maternity and Childcare Hospital, Jinan, People’s Republic of China; 5Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, People’s Republic of China; 6Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250014, People’s Republic of China

Correspondence: Lianjun Li
Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, P. R. China
Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250014, People’s Republic of China
Email lilianjun_jn@126.com

Introduction: Increasing evidence has shown that abnormally expressed long non-coding RNA (lncRNA) plays crucial roles in prostate cancer (PCa) progression.
Materials and Methods: Here, we analyzed the expression level of lncRNA HAND2 antisense RNA 1 (HAND2-AS1) in PCa cells and tissues. Function assays were performed to investigate the biological roles of HAND2-AS1 in PCa cell behaviors. Bioinformatics methods, luciferase activity reporter assay, and RNA pull-down assay were performed to validate the connection of microRNA-106a-5p (miR-106a-5p) with HAND2-AS1. Also, the target of miR-106a-5p was explored using the same methods.
Results: Our results revealed HAND2-AS1 expression was decreased in both PCa cells and tissues. In vitro functional assays showed HAND2-AS1 could inhibit PCa cell proliferation and colony formation through promoting cell apoptosis. Dual-luciferase activity assays showed miR-106a-5p could directly bind with HAND2-AS1 and RNA binding motif protein 24 (RBM24). Mechanistically, we showed that HAND2-AS1 regulates PCa cell behaviors via targeting miR-106a-5p/RBM24 axis.
Conclusion: In summary, our results illustrated that HAND2-AS1 functions as miR-106a-5p sponge to regulate RBM24 expression, and to influence PCa progression.

Keywords: HAND2-AS1, miR-106a-5p, RBM24, prostate cancer

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